Neurospora, mutations

Molecular targets have been elucidated for Dm-AMPl and Rs-AFP2. Dm-AMPl was found to bind plasma membranes from Neurospora crassa and Saccharomyces cerevisiae in a saturable manner and it competed with closely related defensins for binding. Mutational studies with S. cerevisiae identified lipid raffs containing sphingolipids as a molecular target " while glycosylphosphatidylinositol (GPI)-anchored proteins could be... 

In fact, the cross-talk between histone methylation and DNA methylation has been experimentally demonstrated for the first time in Neurospora [145] (see commentary by Bird [146]) and proved the second scenario. Using the power of Neurospora genetics, Tamaru and Selker [145] have identified a gene which, when mutated, abolishes methylation of all tested DNA sequences. This gene, dim-5, is different from dim-2, the previously identified gene that encodes the only DNA methyltransferase in Neurospora responsible for all known cytosine methylation in this fungus. The dim-5 gene encodes a histone H3 methyltransferase the deduced polypeptide sequence contains a SET domain with sequence similarity to some known histone methyltransferases moreover, the recombinant DIM-5 protein exhibits histone methyltransferase activity in vitro. Additional in vivo experiments... 

The gene mutation test in Neurospora crassa US-EPA OPPTS 870.5250... 

The test is used to detect forward and reverse gene mutation in Neurospora crassa, a eukaryotic fungus. These mutations are detected by biochemical or morphological changes in the treated population. The most commonly used mutation assay in Neurospora crassa measures forward mutation in the ad-3 region of the genome. 

Escherichia coli (Sd-4), reverse mutation Klebsiella pneumoniae, forward mutation Schizosaccharomyces pombe, forward mutation Neurospora crassa, reverse mutation... 

Kolmark, G. Giles, N.H. (1955) Comparative studies of monoepoxides as inducers of reverse mutations in Neurospora. Genetics, 40, 890-902 Lide, D.R. Milne, G.W.A. (1996) Properties of Organic Compounds, Version 5.0, Boca Raton, FL, CRC Press [CD-ROM]... 

Pope, S., Baker, J.M. Parish, J.H. (1984) Assay of cytotoxicity and mutagenicity of alkylating agents by using Neurospora spheroplasts. Mutat. Res., 125, 43-53... 

NCR, Neurospora crassa, reverse mutation (+) NT 14000 Kolmark Giles (1955)... 

Kolmark, G. Giles, N.H. (1955) Comparative studies of monoepoxides as inducers of reverse mutations in Neurospora. Genetics. 40, 890-902... 

SCF, Saccharomyces cerevisiae ade2, forward mutation SCF, Saccharomyces cerevisiae ade2, forward mutation SGR, Streptomyces griseoflavus, reverse mutation ANR, Aspergillus chevalieres. reverse mutation NCF, Neurospora crassa, forward mutation NCR, Neurospora crassa, reverse mutation NCR, Neurospora crassa, reverse mutation DMX, Drosophila melanogaster, sex-linked recessive lethal... 

Han, J.-S. (1997) Mutagenic activity and specificity of hydrogen peroxide in the ad-3 forward-mutation test in two-component heterokaryons of Neurospora crassa. Mutat. Res., 374, 169-184... 

Jensen. K.A.. Kirk, L, Koelmark, G. Westergaard, M. (1951) Chemically induced mutations in Neurospora. Cold Spring Harbor Symp. Quant. Biol., 16. 245-261... 

Results of mutation tests with Salmonella typhimurium are inconclusive, but 1,4-benzoquinone does not cause mutations in Neurospora crassa. In cultured mammalian cells, it induced DNA sPand breakage, mutation at the hprt locus and micronuclei. It also induced micronuclei in the bone-marrow cells of mice treated in vivo. Dominant lethal effects were not induced in male mice by a single low dose. 

NCF, Neurospora crassa, forward mutation to pyrimidine - NT NG Reissig (1963) Q... 

Fungi Mutation Neurospora crassa ad-3 system red adenine mutants Gene (forward) mutations and small deletions in ad3A and ad3B <2 mo M M M L... 

Mutation Neurospora crassa Reversion of adenine auxo-trophy in strains N23 and N24 Gene mutations <1 mo L L S NA... 

Katz, T-M. Ong, A.J.F. Griffiths, and R.S. Stafford. Mutation tests in Neurospora crassa. A report for the U.S. EPA GENE-TOX Program. Mutat. Res. (in press)... 

Ong, T-M. Use of the spot, plate and suspension test systems for the detection of the mutagenicity of environmental agents and chemical carcinogens in Neurospora crassa. Mutat. Res. 53 297-308, 1978. 

A few examples include the mouse specific-locus test for germ-cell mutagenesis (Russell 1994 Russell 1989), tests for forward and reverse mutations in the ad-3 region of Neurospora crassa (de Serres and Kolmark 1958), the Tradescantia stamen-hair system (Underbrink et al. 1973), and the rlllocus test for bacteriophage T4 (Drake 1963). Many others could be noted. 

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