Schizosaccharomyces pombe

A wide range of mammalian proteins have been expressed in S. pombe. In a successful example, the human lipocortin I comprised 50% of the total soluble proteins in yeast cells and showed high activity, indicating that the post-translational modifications were mammalian-like [104]. Membrane proteins including cytochrome P450 were expressed at ten times the levels of those in other yeast systems [105]. Also, GPCRs have been expressed in S. pombe, where the human dopamine D2 receptor was correctly inserted into the yeast cell membrane and demonstrated expression levels three times those of S. cerevisiae [106]. 

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Degraeve N, Gilot-Delhalle J, Moutschen J, et al. 1980. Comparison of the mutagen activity of organophosphorus insecticides in mouse in the yeast schizosaccharomyces pombe. Mutat Res 74 201-202. 

Schizosaccharomyces pombe (mouse host-mediated Gene mutation - Rossi et al. 1983 ... 

Traditional and well-established yeast species are Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Pichia pastoris and Schizosaccharomyces pombe. With every year that passes they are increasingly being used in industrial and pharmaceutical enzyme production on a large scale. Many further yeasts present interesting features (e.g. Arxula adeninivorans and Yarrowia lipolytica), but are not that widely used. 

Ikeda, S., Nikaido, K., Araki, F K. et al. (2004) Production of recombinant human lysosomal acid lipase in Schizosaccharomyces pombe. development of a fed-batch fermentation and purification process. Journal of... 

Toyama, R. and Okayama, H. (1990) Human chorionic gonadotropin alpha and human cytomegalovirus promoters are extremely active in the fission yeast Schizosaccharomyces pombe. FEBS Letters, 268 (1), 217-221. 

Sarmah, B., Chakraborty, N., Chakraborty, S. and Datta, A. (2002) Plant pre-mRNA splicing in fission yeast, Schizosaccharomyces pombe. Biochemical and Biophysical Research Communications, 293 (4), 1209—1216. 

Schizophrenic block copolymers, 20 488 Schizophillan, 20 578 Schizosaccharomyces pombe genome of, 26 450t Schlack, Paul, 19 739 Schlegel diagrams, 12 236, 238-241, 244 Schlippe s salt, 3 65 Schmidt and Launsby, important... 

Hildebrandt, V. In Foreign Gene Expression in Fission Yeast Schizosaccharomyces pombe Giga-Hama, Y., Kumagai, H., Eds. Springer Verlag Berlin, 1997 pp 97-110. 

Yen, H. C., Gordon, C., and Chang, E. C. Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome. Cell 2003, 112, 207—17. 

Wood V, GwilUam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S et al (2002) The genome sequence of Schizosaccharomyces pombe. Nature 415 871—880... 

Phosphorylation of ubiquitin ligases could have inhibitory effect as well. In the fission yeast Schizosaccharomyces pombe, PKA blocks APC activity. Moreover, the inhibitory effect of PKA seems to be dominant over the stimulatory effect of the polo-like kinase. Even if APC has been activated by polo-like kinase, addition of mammalian PKA to APC-containing fractions inhibits ubiquitination of the substrate cyclin... 

Wu G, Mansy SS, Hemann C, et al. 2002a. Iron-suHur cluster biosynthesis Characterization of Schizosaccharomyces pombe Isal. J Biol Inorg Chem 7 526-32. 

Wu G, Mansy SS, Wu S-P, et al. 2002b. Characterization of an iron-sulfur cluster assembly protein (ISUl) from Schizosaccharomyces pombe. Biochemistry 41 5024-23. 

Hadwiger, J. A., Wittenberg, C., Mendenhall, M. D., and Reed, S. I. (1989). The Saccharomyces cerevisiae CKSl gene, a homolog of the Schizosaccharomyces pombe sucl-t- gene, encodes a subunit of the Cdc28 protein kinase complex. Mol Cell Biol 9, 2034 1. 

Richardson, H. E., Stueland, C. S., Thomas, J., Russell, P., and Reed, S. I. (1990). Human cDNAs encoding homologs of the small p34cdc28/cdc2-associated protein in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Genes and Dev 4, 1332-1334. 

The resultant master ORF clones should be checked by several different criteria DNA sequence, protein productivity, and the structure of produced proteins see Note 5). Once these master ORF clones whose quality has been well-checked become available to the research community, a wealth of time, labor, and cost will surely be saved and provide a solid basis for reverse chemical genomic approaches. In this context, many groups working on ORFeome cloning have made their ORF clones open to the research community For human ORF clones, the ORFeome collaboration is actively working to generate a complete set of human ORF clones as an international collaboration (http // www.orfeomecollaboration.org/) Schizosaccharomyces pombe ORF clones are already available at the DNA bank of RIKEN... 

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