Neurospora

The use of mutant 34486 of Neurospora crassa for the microbiological assay of ch oline has been described (8). A physiological method has also been used in which the ch oline is extracted after hydrolysis from a sample of biological material and acetylated. The acetylcholine is then assayed by a kymographic procedure, in which its effect in causing contraction of a piece of isolated rabbit intestine is measured (33). 

Na, K -ATPase, a K ATPase Ca-+-ATPase, SR Ca" -ATPase, plasma membrane H -ATPase, yeast H" -ATPase, Neurospora... 

A portion of the DNA sequence of nit-6, the Neurospora gene encoding the enzyme nitrite reductase. (JaviesD. Cotandene, University of Virginia ... 

Nerve growth factor snake venoms zinc, 6, 613 Neurospora crassa calcium transport, 6, 571 cation transport, 6, 559 Neurosporin, 6, 676 Neurotransmitters secretion calcium, 6, 595 Neutral complexes electrical properties, 6, 143 Neutron absorbers... 

The molybdenum cofactor was liberated from D. gigas AOR, and under appropriate conditions was transferred quantitatively to nitrate reductase in extracts of Neurospora crassa nit-1 mutant) to yield active nitrate reductase 217). On the basis of molybdenum content, the activity observed for reconstitution with molybdenum cofactor of D. gigas was lower (25%) than the values observed for the procedure using extractable molybdenum cofactor of XO, used as reference. This result can now be put in the context of the difference in pterin present (MPT-XO and MCD-AOR) 218). 

Neurospora crassa is a filamentous pink mould. It became famous to scientists due to the work of Beadle and Tatum in the 1940s when they developed the one gene—one enzyme hypothesis. Its life cycle is shown in Fig. 2.9. Unforturrately, the full force of fungal nomenclature comes into play when considering this organism. Aerial hyphae... 

E. Physical Methods and Analytical Techniques.—Nucleotide maps of enzymic digests of DNA have been obtained using the same ionophoretic techniques as have been developed for RNA digests. Pancreatic DNase and Neurospora crassa endonuclease produce very similar maps with E. coli DNA but this technique still awaits the discovery of specific DNases. 

Goldie, A.H. and Subden, R.E., The neutral carotenoids of wild-type and mutant strains of Neurospora crassa, Biochem. Genet. 10, 275, 1973. 

Hausmann, A. and Sandmann, G., A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa, Eungal Genet. Biol. 30, 147, 2000. 

Carotene Yellow-orange Neurospora crassa (fungus) RP... 

Purification and characterisation of galactose-induced pectinases from the exo-1 mutant strain of Neurospora crassa... 

E. coll Chlorella Arabidopsis Synechocystis Leishaania Neurospora qa-y E. coll paH322 E. coli CIT E. coli LacY... 

E. coli AraE E. coli XylE E. coli GelP Chlorella Arabidopsis Synechocystis Leishmania Neurospora qa-y e. coli pBRl22 E. coli CIT E. coli I CY... 

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