Trichothecenes mycotoxins

In addition to their impacts as mycotoxins, trichothecenes are also known as phytotoxins for the toxin-producing phytopathogenic fungi 230 Above all, Fusarium species, such as F. graminearum and F. culmorum, are the problematic fungal species producing trichothecenes because they are the causal pathogens of Fusarium head... 

In the years 2007, 2008, and 2009, altogether 175 samples of cereals produced in Slovenia were collected at farms, among them 79 samples of maize, 39 samples of barley, and 34 samples of wheat. In the samples, among analysed mycotoxins, trichothecenes were determined using the analytical procedure mentioned above. 

This same experimental approach can be used to determine the appHcabiUty of the aDAS—AP to a competitive assay for DAS. As shown in Eigure 6, increasing amounts of free DAS were used to define the 50% inhibition level (ID q) of DAS for binding of two aDAS—AP conjugates to immobilized DAS. This approach was also used to determine the sensitivity of an EIA, as well as the specificity of the assay, as shown in Table 2. Increasing amounts of trichothecene mycotoxins closely related to DAS were added to microtiter plate wells containing a constant amount of prereacted DAS—aDAS—AP. After 30 min, excess toxin and any free toxin—aDAS—AP were washed out, and substrate was added. Quantification of the color produced was directly related to the abihty of the added toxin to displace aDAS—AP from the immobilized DAS, which is an indication that the aDAS also has an avidity for that toxin. 

Reagent for Epoxides e.g. trichothecene-mycotoxins [1 — 6] valepotriates [7,17] Olefins, acetylene derivatives [8] 4-Hydroxycumarin, anthraquinone [8] Alkylating agents [9-12] NOz... 

Of four basic trichothecene groups (A, B, C and D), types A and B represent the most important mycotoxins. Type A includes the T-2 toxin that can suppress the immune system and can cause damage to bone marrow. The T-2 toxin is about 14 times more toxic than the most widespread Fusarium-based type B mycotoxin, deoxynivalenol (DON), previously called vomitoxin. DON is a... 

WHO (1990), Selected mycotoxins ochratoxins, trichothecenes, ergot , Environ, Health Criteria 105, Geneva, Switzerland, WHO, 1-163. 

Modulation of Inflammatory Gene Expression by Trichothecene Mycotoxins... 

Bamburg, J. R. Biological and Biochemical Actions of Trichothecene Mycotoxins. Prog. Mol.randSubcel.rBiol. 8,41, 1983. 

Heyndrickx, A., Sookvanichsilp N., and Van-den H.M. Detection of trichothecene mycotoxins (yellow rain) in blood, urine and faeces of Iranian soldiers treated as victims of a gas attack. Arch. Belg. Suppl, 143, 1984. 

Yang, G. et al. Apoptosis induction by the satratoxins and other trichothecene mycotoxins Relationship to ERK, p38 MAPK and SAPK/JNK Activation. Toxicol. Appl. Pharmacol. 164, 149-160, 2000. 

Shifrin, V. I. and Anderson P. Trichothecene mycotoxins trigger a ribotoxic stress response that activates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase and induces apoptosis. J. Biol. Chem. 274, 13985, 1999. 

Jemmali M, Ueno Y, Ishii K, Frayssinet C, Etienne M (1978) Natural occurrence of trichothecenes, nivalenol, deoxynivalenol, T-2 toxin and zearalenone in corn. Experientia 34 1333-1334 Josephs RD, Schuhmacher R, Krska R (2001) International interlaboratory study for the determination of the Fusarium mycotoxins zearalenone and deoxynivalenol in agricultural commodities. Food Addit Contam 18 417-430... 

Biological Agents BACTERIA (Anthrax, Brucellosis, Cholera, Plague, Tularemia). VIRUSES (Crimean-Congo Hemorrhagic Fever, Rift Valley Fever, Smallpox, Venezuelan Equine Encephalitis (VEE), Viral Hemorrhagic Fever (Ebola)). TOXINS (Botulinum, Ricin, Staphylococcal Enterotoxin B (SEB), Trichothecene Mycotoxins/T-2). 

Yellow Rain A lethal yellow substance thought to have been dispersed aerially as a warfare agent in Southeast Asia and Afghanistan the lethal component is though to have been a trichothecene mycotoxin that was reported to produce severe nausea and vomiting, disturbances in the central nervous system. Fever, chills, and abnormally low blood pressure with a case mortality of approximately 50 percent. 

Trichoderma reesi, 12 479, 480 Trichoderma viride, 10 536 Trichothecene mycotoxins, 14 144, 146 Trichromatic sources, for white LEDs, 14 862... 

Trichothecene mycotoxin Toxin produced by fungal molds it inhibits protein synthesis, impairs DNA synthesis, and interferes with cell membrane structure and function. 

This section addresses the toxin forms of biological agents that would most likely be considered by terrorists. These agents include C. botulinum, C. perfringens, S. enterotoxin B, ricin, saxitoxins, and trichothecene mycotoxins (T-2). 

Trichothecene mycotoxins are produced by a number of fungal molds of the Fusarium, Myrotecium, Trichoderma, and Stachybotrys genera. They inhibit protein synthesis, impair DNA synthesis, and interfere with cell membrane structures and functions. The potential routes of exposure are inhalation, ingestion, and skin absorption. A terrorist may take advantage of any of these routes. 

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