Mycobacterium tuberculosis culture

Rosenkrands, I., Rasmussen, P.B., Camio, M., Jacobsen, S., Theisen, M., and Andersen, P. 1998, Identification and characterization of a 29-kilodalton protein from mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. Infect Immun 66 2728-2735. 

Sonnenberg, M.G. and Belisle, J.T. 1997, Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry. Infection Immunity 65 4515-4524. 

Division T.C.T., Tuberculosis Research Centre C., 2009 (Indian C. of M.R., and National Tuberculosis Institute, Bangalore (Directorate General of Health Services G. of I. 2009. Revised National TB Control Programme Training Manual for Mycobacterium Tuberculosis Culture Drug Susceptibility Testing. New Delhi. 

Wedlock, D.N. Keen, D.L. McCarthy, A.R. Andersen, P. Buddie, B.M. (2002). Effect of different adjuvants on the immune responses of Mycobacterium tuberculosis culture filtrate proteins, Vet Immunol Immunopathol, 86, 79-88. 

Drobniewski E.A., More P.G., Harris G. S. Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence in situ hybridization probes./. Clin. Microbiol. 2000 38 444-447. 

Twenty-five dibenzothiophenes, composed mainly of amino and acetamidodibenzothiophenes and their sulfoxides and sulfones, have been tested for antibacterial activity against Staphylococcus aureus, Escherichia coli, and Mycobacterium tuberculosis. Activity was found against the latter with 2- and 3-aminodibenzothiophene in synthetic culture media, but the effect was reversed by the addition of serum. " ... 

Streptomycin (Figure 8.25) is produced by cultures of a strain of Streptomyces griseus, and is mainly active against Gram-negative organisms. Because of its toxic properties it is rarely used in modern medicine except against resistant strains of Mycobacterium tuberculosis in the treatment of tuberculosis. 

Mycobacterium tuberculosis. Many polysaccharides from this organism have been studied10 and have been isolated from the culture medium, the somatic portion of the cell, and the cell lipoids. Only in a few cases have the structures been definitely established. 

G Harth, MA Horwitz (1999) An inhibitor of exported Mycobacterium tuberculosis glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes extracellular proteins as potential novel drug targets, J Exp Med 189(9) 1425-1436... 

Based on the patient s history and lab information, he was started on a regimen of isoniazid, rifampin, pyrazinamide, and ethambutol while waiting for definitive cultures. (Six weeks later, definitive culture results demonstrated growth of Mycobacterium tuberculosis.) The patient returned to the clinic complaining of blurred vision and an inability to differentiate between green and red colors. 

JS, a 46-year-old male patient, returned from India with cough, malaise, weight loss and night sweats. Sputum culture showed acid-fast bacilli and 3 days later Mycobacterium tuberculosis was isolated. 

Along with Mycobacterium tuberculosis H37Rv, several clinical isolates of Mycobacterium tuberculosis were employed in this work. The MIC was defined as the lowest concentration of the drug in mg/L, which inhibited more than 99% of the population in the control culture on the seventh day. 

Armstrong JA, Hart DPA. Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes. Exp Med 1971 134 713-740. 

A 72-year-old man with a normal chest X-ray was unnecessarily treated with G-CSF (5 micrograms/kg/ day) for very moderate cytopenia. Five days later, he complained of dyspnea and fatigue, but without fever. His chest X-ray showed diffuse bilateral alveolar opacities and he had a low oxygen saturation. Blood cultures were negative, and infectious pneumonitis (with Mycobacterium tuberculosis, Pneumocystis proved, Herpes simplex, and cytomegalovirus) was ruled out. Despite glucocorticoid and antibiotic treatment, he required mechanical ventilation and died 12 days after the onset of symptoms. 

Polysaccharides which resemble animal glycogens in chemical and physical properties have been isolated from bacterial cells, including avian and human strains of Mycobacterium tuberculosis and surface cultures of enteric bacteria (for example, Escherichia coli and Salmonella monte-video), and also from yeasts. ... 

Mycobacterium tuberculosis Tuberculosis More rapid than culture, possibly more sensitive less hazardous in lab B5, H2, S10... 

Bioassay is the quantitative method in which the endpoint is an observable effect on a biological system or an organism. The classical approach to microbial detection involves the use of differential metabolic assays (monitored colormetrically) to determine species type in the case of most bacteria, or the use of cell culture and electron microscopy to diagnose viruses and some bacteria that are intracellular parasites. Samples taken from the environment, such as soil and water, and most clinical samples must be cultured in order to obtain sufficient numbers of various cell types for reliable identification. The time required for microbial outgrowth is typically 4-48 hours (or two weeks for certain cases, such as Mycobacterium tuberculosis). Furthermore, bacterial culture suffers from an inherent drawback cells that are viable may not be culturable, because they possess unanticipated nutritional requirements as a result of genetic mutation. 

G9. Goto, M., Shinichi, O., Okuzumi, K., Kimura, S., and Shimada, K., Evaluation of acridinium ester-labeled DNA probes for the identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture. J. Clin. Microbiol. 29, 2473-2476 (1991). 

It has been discovered by a number of researchers " , that microorganisms generally develop a limited degree of resistance to the nitrofurans. Micro-organisms that are transferred repeatedly into culture media containing nitrofurazone require higher minimal inhibitory concentrations later on. The increased concentration is usually 10 times, rarely 50 times higher. There are, however, exceptions resistance of Mycobacterium tuberculosis to nitrofurazone and Staphylococcus aureus to nitrofurantoin were observed in vitro. Repeated transfers of Escherichia coli will increase the minimal inhibitory concentration of nitrofurantoin by about 35 times. Much work... 

Vitamin Bl4 and Vitamin B , Two factors reported to be necessary for growth and feathering of chicks Briggs er al. J. Biol. Chem. 148, 163 (1943). Prepn from culture filtrates of Mycobacterium tuberculosis Mills et al, Proc. Soc. Exp. Biol. Med. 56, 240 (1944). 

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