Terminal amino acid sequence

ECEs are metalloproteinases that are homologous to the neutral endopeptidase (NEP, E-24.11, neprilysin) unlike NEP, however, they form disulfide-bonded homodimers. In man, with ECE-1 and ECE-2, two isoforms are known, which are encoded by two separate genes. For ECE-1 four different variants have been identified (ECE-1 a-d), which are generated by the use of alternative promoters (Table 2). The ECE-1 isoforms only differ in their N-terminal amino acid sequence. For ECE-2, a single gene product has been described in... 

The common C-terminal amino acid sequence required for exerting activity at tachykinin receptors is shown in bold endokinin C and D lack the C-terminal Met and are almost devoid of affinity at these receptors. In red, the sequence of neurokinin A of which neuropeptide-gamma and neuropeptide-kappa are elongated forms and neurokinin A (3-10) is a product of beta or gamma-TAC1 mRNAs or an NKA metabolite active at tachykinin receptors. In blue, the sequence of human HK-1 of which endokinin A and B are elongated forms. 

The calculated value for the Hisg-tagged protein is 28,492 Da. N-terminal amino acid sequence analysis (Procise) was carried out on purified recombinant resilin. The following sequence (at 120 pmol yield) was obtained for the first 12 amino acid residues MHHHHHHPEPPV, as expected from DNA sequence analysis. 

Stoichactis (Stichodactyla) helianthus. It has recently been shown by CM-cellulose chromatography that heliantholysin consists of four isotoxins having different N-terminal amino acid sequences (Kern and Dunn, in press). Designated I to IV in order of increasing isoelectric pH, toxins I and II had one additional amino acid at the amino terminus than did toxin III. Toxin IV had a seven residue extension at the amino terminus relative to toxin III. Toxin HI and toxin II contributed 83% and 14% of the total hemolytic activity, respectively, and toxins I and FV together about 3%. 

A Pstl-HinA (1.2 kbp) fragment was subcloned in pUC19. The enzyme produced by the E. coli transformant was purified fo homogeneify and shown to be identical to that of the original strain. Both enzymes had the same enzymological properties and N-terminal amino acid sequences. ... 

Although it had been assumed that only hypoxanthine dehydrogenase is required for the conversion of hypoxanthine (6-hydroxypurine) into uric acid, in Clostridium purinolyti-cum, two enzymes, both of which contain a selenium cofactor, are required. The enzymes differ in the molecular mass of their subunits, in their terminal amino acid sequences, in their kinetic parameters, and in their specific activities for purines (Self and Stadman 2000). Purine hydroxylase converts purine into hypoxanthine and xanthine (2,6-dihy-droxypurine), which is then further hydroxylated to uric acid (2,6,8-trihydroxypurine) by xanthine dehydrogenase (Self 2002). 

The goals of the project were to confirm the molecular weight of hementin, determine the N-terminal amino acid sequence, and provide sufficient purified protein for biochemical studies of the fibrinolytic activity. These goals were all attained. Many of the issues that become important in devising a scalable process were identified, particularly... 

Christie, J.F., Dunbar, B., Davidson, I. and Kennedy, M.W. (1990) N-terminal amino acid sequence identity between a major allergen of Ascaris lumbricoides and Ascaris suum, and MHC-restricted IgE responses to it. Immunology 69, 596-602. 

Imataka, H., Gradi, A., and Sonenberg, N. (1998). A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation. EMBOJ. 17, 7480-7489. 

T. Happe, JD. Naber (1993) Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii. Eur. J. Biochem., 214 475-481... 

Using the ethyl 1 -thio derivative (97) of 2,3,4-tri-O-benzoyl-D-xylose, the fully protected and free O-glycopeptides 99 and 100, having the TV-terminal amino acid sequence 3 to 6 (98) of the protein core of a proteodermatan sulfate have been prepared (69). 

Niu, C. I., and H. Fraenkel-Conrat C-terminal amino acid sequence of tobacco mosaic virus protein. Biochim. Biophys. Acta 16, 597-598 (1955). 

Signal peptide an N-terminal amino acid sequence that targets preproteins to membrane sites. 

Fig. 1. Core histone modifications. Human histone N-terminal and in some cases C-terminal amino acid sequences are shown. The modifications include methylation (M), acetylation (Ac), phosphorylation (P), ubiquitination (U), and ADP ribosylation (step ladder). The sites of trypsin digestion of histones in nucleosomes are indicated (T).

AM-001, and mannanase B properties are similar to those of P-mannanase M-III. Furthermore, the Ouchterlony double diffusion test showed that these five enzymes gave fused precipitation lines. However, N-terminal amino acid sequences of the five mannanases determined by an automatic amino acid sequencer revealed that the N-terminal amino acid sequence from amino acid 1 (Asn) to 9 (Gin) of the Bacillus sp. AM-001 enzymes coincides with those from amino acid 4 (Asn) to 12 (Asn) of the R coll JMlOl (pMAH3) enzymes as shown in Fig. 4. This may reflect differences in the specificities of the signal peptidases of the two bacteria. 

FTase catalyzes the covalent attachment of a farnesyl moiety via a thioether Unkage to the proteins bearing a C-terminal amino acid sequence known as the CAAX motif (Fig. 2) [12,21]. The farnesyl moiety is derived from farnesyl pyrophosphate (FPP), a 15-carbon isoprenyl intermediate in the mevalonate pathway of cholesterol biosynthesis. The binding of FPP to the enzyme has relatively high affinity (K = 1-lOnM), and FPP binding must precede the binding of the peptide substrate for successful catalysis [22,23]. 

In the event that no partial DNA sequences are known, but functional target protein is available, we can identify the terminal amino acid sequence. The three-to-... 

There has been no research reported on the interaction of periplanones with pheromone-binding proteins or dendritic receptors. Nevertheless, two studies, one empirical and one theoretical, report on intrasensillar events in P. americana. Picim-bon and Leal (1999) determined the N-terminal amino acid sequences of two soluble proteins (putative odorant-binding proteins) that are specifically expressed in male antennae, and several other proteins that are expressed in antennae of both males... 

Gas-liquid chromatography used for the determination of C-terminal amino acids and C-terminal amino acid sequences in nanomolar amounts of proteins was described in 1976 by Davy and Morris. Based on carboxypeptidase A digestion of the protein, the partially digested protein was removed and the amino acids released after known time intervals were analyzed by quantitative gas-liquid chromatography. Sequences deduced from the kinetics of release of specific amino acids are compared with the known C-terminal sequences of well-characterized proteins. Thus the amino acid sequences were determined. 

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