Mouse testis

Russell JJ, Lindenbaum A, Grahn D. 1979. The micro distribution, retention, and autoradiography of 241Am-citrate in mouse testis. Health Phys 37(6) 831-832. 

B-raf, a member of the raf gene family of serine/threonine kinases, is expressed as two major transcripts of 4.0 kb and 2.6 kb in the mouse testis (Wadewitz et al., 1993). B-raf expression is limited to the germ cells and is particularly abundant in early spermatids. Northern hybridization analysis revealed that the two B-ra/transcripts are expressed in a stage-specific manner. Low levels of the 4.0-kb transcript are first... 

Wadewitz, A. G., Winer, M. A., and Wolgemuth, D. J. (1993). Developmental and cell-lineage specificity of ra/family gene expression in mouse testis. Oncogene 3 1055-1062. 

Crotonaldehyde was formed NADPH-dependently as a minor metabolite of butadiene (partial pressure of 48-52 cm Hg = 660 000 ppm) in microsomes obtained from liver, lung or kidney of male B6C3Fi mice (Sharer et al., 1992) or human liver (Duescher Elfarra, 1994), the formation rate being 20-50 times lower than that of epoxybutene. 3-Butenal was suggested as an intermediate metabolite. No crotonaldehyde formation was observed with microsomes from mouse testis or with microsomes of testis, liver, lung or kidney of male Sprague-Dawley rats (Sharer et al., 1992). 

SHL, Sister chromatid exchange, human lymphocytes in vitro DVA, DNA strand breaks, CD-I mouse testis in vivo... 

Koivisto, P, Adler, I.-D., Pacchierotti, F. Peltonen, K. (1998) DNA adducts in mouse testis and lung after inlralation exposure to 1,3-butadiene. Mutat. Res.. 397, 3-10... 

Jackson et al. (1988) were the first to employ the immunogold-silver staining (IGSS) method in combination with microwave heating. They completed within minutes the incubations in primary and secondary antibodies for detecting immunoglobulins in paraffin sections of human tonsil, van de Kant et al. (1990) applied the same method, except that resin instead of paraffin sections were used to detect bromodeoxyuridine incorporated in cells of the mouse testis. Tissue morphology is preserved better in a resin than in paraffin. The former also allows the use of thinner sections. 

Sarge, K.D., Park-Sarge, O.Y., Kirby, J.D., Mayo, K.E. and Morimoto, R.I. (1994) Regulated expression of heat shock factor 2 in mouse testis potential role as a regulator of HSP gene expression during spermatogenesis. Biol. Reproduct., 50, 1334-1343. 

Muramatsu, T., Shibata, O., Ryoki, S., Ohmori, Y. and Okumura, J. (1997) Foreign gene expression in the mouse testis by localized in vivo gene transfer. Biochem. Biophys. Res. Commun., 233, 45—49. 

IIC Rat/mouse testis 14.6s 1 (human) and distal part of chromosome 4 (mouse)... 

R9. Russo, J., Subcellular distribution of acid phosphatase in the mouse testis. Ada Physiol. Lat. Amer. 20, 78-80 (1970). 

Hasegawa M, Wilson G, Russell ED, Meistrich ML. 1997. Radiation-induced cell death in the mouse testis relationship to apoptosis. Radiat. Res. 147 457-67... 

Cotman, M., D. Rozma, L. Banek, and D. Jezek (2001). Localisation of lanosterol 14a-demethylase in round and elongated spermatids of the mouse testis An immunoelectron microscopic and stereo-logical study. PJlugers Arch. 442, R167-R168. 

Falciatori I, Borsellino G, Haliassos N, et al. (2004). Identification and enrichment of spermatogonial stem cells displaying side-popnlation phenotype in immatnre mouse testis. Faseb. J. 18 376-378. 

Lassalle B, Bastos H, Louis J P (2004). Side Population cells in adult mouse testis express Bcrpl gene and are enriched in spermatogonia and germinal stem cells. Develop. 131 479-487. 

Figure 4 Triantennary iV-glycans involved in germ cell and Sertoli cell interaction in the mouse testis. Spermatogenesis takes place in the tubal structure called seminiferous tube in the testis. Inside of seminiferous tube is lined by Sertoli cells, the tail epithelial cells. When germ stem cells at the base of Sertoli cells differentiate to spermatocytes, the cells move upward interacting with Sertoli cells. The MX gene knockout mouse revealed that fucosylated GlcNAc-terminated triantennary A-glycan (insert) plays an important role in germ cell adhesion to Sertoli cells (59).

Lee MC, Damjanov I. Anatomical distribution of lectin-binding sites in mouse testis and epididymis. Differentiation 1984 27 74-81. 

Chou CC, Lou YC, Tang TK et al (2010) Stmcture and DNA binding characteristics of the three-Cys(2)His(2) domain of mouse testis zinc finger protein. Proteins 78 2202-2212... 

Ol. Odet, F., Guyot, R., Leduque, P., and Le Margueresse-Battistoni, B., Evidence for similar expression of protein C inhibitor and the urokinase-type plasminogen activator system during mouse testis development. Endocrinology 145, 1481-1489 (2004). 

Demerec M (1950) Biology oi Drosophila, Wiley, New York/Chapman Hall, London Distel RJ, Kleene KC, Hecht NB (1984) Haploid expression of a mouse testis. -tubulin gene. Science 224 68-70... 

Kawamata, M. Inoue, H. Nishimori, K (2007). Male-specific function of DMRT7 by sexually dimorphic translation in mouse testis. Sexual Development, Vol.l, No.5, p>p. 297-304, ISSN 1661-5425... 

Chieffi P, Barchi M, Di Agostino S, Rossi P, Tramontano D, Geremia R. 2003. Prolin-rich tyrosine kinase 2 (PYK2) expression and localization in mouse testis. Mol Reprod Dev 65(3) 330-335. 

Small pieces of adult mouse testis are placed in Eagle s medium with 2% calf serum (6 testes/50ml) and stirred for 10 min to liberate the cells. The medium is then filtered to remove pieces of tissue. The filtrate, containing the isolated cells, is preincubated (Ih at 37°C in an atmosphere of 93.5% Oi-6.5% CO2) in a shaker at low speed. The cell dispersion is then placed in ice and centrifuged for 5 min at 4°C at low speed. The supernatant is discarded and the cells are resuspended in Eagle s medium containing 2% calf serum. 

Poly(ADP-ribose) synthetases were purified from calf thymus [4], mouse testis [5], and human placenta [6]. Proteolytic digestion was performed as described previously [7, 8]. 3-(Bromoacetyl)pyridine was prepared according to the method of Woenckhaus... 

Poly(ADP-ribose) synthetases were purified to apparent homogeneity from calf thymus, mouse testis, and human placenta. The major characteristics of these enzymes are presented in Table 1. In addition to molecular mass, sedimentation constant, isoelectric point, and partial specific volume, the apparent for NAD and DNA as well as V ,ax of the reaction are all common to these three enzymes. Amino acid compositions of the enzymes are shown in Table 2. Here again, the numbers of each amino acid residue are very similar to each other, although some differences as denoted by star symbols are noted. 

Fig. 1. Interspecies cross-reactivity of poly(ADP-ribose) synthetases as detected by immunoblotting. DNA binding proteins prepared from calf thymus (lane 7), HeLa cells (lane 2), mouse testis (lane 3), and chicken liver (lane 4) were immunostained with antisera against calf thymus poly(ADP-ribose) synthetase after SDS gel electrophoresis and trans-blotting...

Momii A, Koide SS (1980) RNA synthesis and poly(ADP-ribose) synthetase activity in developing mouse testis. Fed Proc 39 954... 

Poly(ADP)-Ribosylation of Nuclear Proteins in the Mouse Testis... 

We have reported previously [8] that in mouse testis non-histone proteins are labelled to a higher specific activity than histones following an i.p. injection of ribose and [ H]-adenine, and that the radioactivity is covalently bound to protein in the form of ADP-ribose. This was in contrast to the situation in liver where histones incorporated more radiolabel than non-histone proteins. We have recently extended these observations on ADP-ribosylation of nuclear proteins in testis [9] and we report... 

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