Poly synthetase mouse testis

Poly(ADP-ribose) synthetases were purified from calf thymus [4], mouse testis [5], and human placenta [6]. Proteolytic digestion was performed as described previously [7, 8]. 3-(Bromoacetyl)pyridine was prepared according to the method of Woenckhaus... 

Poly(ADP-ribose) synthetases were purified to apparent homogeneity from calf thymus, mouse testis, and human placenta. The major characteristics of these enzymes are presented in Table 1. In addition to molecular mass, sedimentation constant, isoelectric point, and partial specific volume, the apparent for NAD and DNA as well as V ,ax of the reaction are all common to these three enzymes. Amino acid compositions of the enzymes are shown in Table 2. Here again, the numbers of each amino acid residue are very similar to each other, although some differences as denoted by star symbols are noted. 

Fig. 1. Interspecies cross-reactivity of poly(ADP-ribose) synthetases as detected by immunoblotting. DNA binding proteins prepared from calf thymus (lane 7), HeLa cells (lane 2), mouse testis (lane 3), and chicken liver (lane 4) were immunostained with antisera against calf thymus poly(ADP-ribose) synthetase after SDS gel electrophoresis and trans-blotting...

Momii A, Koide SS (1980) RNA synthesis and poly(ADP-ribose) synthetase activity in developing mouse testis. Fed Proc 39 954... 

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