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DNA from calf thymus

As the results of Schwander and Cerf (188) and of Leray (180,181), which were obtained on samples of DNA from calf thymus, have been reproduced already in several review articles (1,3), the present discussion can be kept rather short. When the viscosity of the solvent (1.0 molar aquous solution of sodium chloride) is increased by replacing part of the water by glycerol, the behaviour of the initial slope of the extinction angle curve follows the qualitative pattern, as given by Fig. 5.10. At low solvent viscosities the molecules seem to behave like frozen molecules, at high solvent viscosities they seem to become flexible coils exhibiting internal friction. These results have been considered to prove the correctness of Cerf s theoretical model. [Pg.285]

Highly-polymerized deoxyribonucleic acid, (DNA), from calf-thymus, was purchased from Sigma Chem. Co. and used as received. Stock solutions of DNA were prepared by dissolution overnight in 5 mM phosphate pH 7 buffer and were stored at 4°C in the dark for short periods only. Concentrations of DNA per nucleotide phosphate were determined by absorption spectroscopy using a molar extinction coefficient of 6,600 M cm at 260 nm [4]. N,N -Dimethyl-2,7-diazapyrenium dlchloride (DAP " ") was prepared and purified according to the method of HUnig et al. [5] whereas ethidium bromide (EB ) was purchased from Sigma Chem. Co. and used as received. [Pg.396]

Zaitsev, S.V., Haberland, A., Otto, A., Vorob ev, V.I., Haller, H., Bottger, M. (1997). HI and HMG17 extracted from calf thymus nuclei are efficient DNA carriers in gene transfer. Gene Ther., 4, 586-592. [Pg.370]

Tretyakova, N., Lin, YP, Sangaiah, R., Upton, PB. Swenberg, J.A. (1997a) Identification and quantitation of DNA adducts from calf thymus DNA exposed to 3,4-epoxy-l-butene. Carcinogenesis, 18, 137-147... [Pg.222]

During the middle phase the composition of the 3 -P terminal and of the 5 -OH terminal and penultimate nucleotides of oligonucleotides obtained from calf thymus DNA by acid DNase digestion, in the incubation conditions of Fig. 1, is practically constant. Some results obtained in this phase are given in Table III. Purine nucleotides form about 75% of the 3 -terminals and of the 5 -OH penultimates with a predominance of G in... [Pg.284]

DNA Extraction from Calf Thymus or Herring Sperm... [Pg.168]

Cary, P. D., King, D. S., Crane-Robinson, C., Bradbury, E. M., Rabbani, A., Goodwin, G. H., and Johns, E. W. (1980). Structural studies on two high-mobility-group proteins from calf thymus, HMG-14 and HMG-20 (ubiquitin), and their interaction with DNA. Eur. J. Biochem. 112, 577-580. [Pg.327]

Two examples of Py-MS spectra dominated by the ions generated from the sugar moiety are the spectrum for a DNA sample from calf thymus obtained by Curie point Py-MS and El at 14 eV [8] (shown in Figure 13.2.1) and the Py-MS spectrum for a RNA sample from baker s yeast generated in the same experimental conditions [8] (shown in Figure 13.2.2). [Pg.403]

Figure 13.2.1 Py-MS spectrum for a DNA sample from calf thymus obtained with 14eV El [8]. Figure 13.2.1 Py-MS spectrum for a DNA sample from calf thymus obtained with 14eV El [8].
Naturally, the next application was aimed at the quantitation of this relationship. The ratio of 5-methylcytosine cytosine in DNA was determined by mass spectrometry, using multiple selected ions monitoring, for (/)-174 DNA as well as for DNA originating from calf thymus, salmon sperm, and several other sources. It must be noted however, that these GC-MS data were obtained from hydrolysates of the whole DNA. [Pg.99]

Fig. 22. Effect of tilorone on the Tm with DNA preparations from various sources of different base compositions. Tm values were obtained at a molar ratio of drug to DNA-P of 0,21. The Tm of DNA (5 x 10 SM) with and without tilorone was determined in a medium of 0.01 M Tris-HCl (pH 7.0). AT-content of B. cereus DNA (65%), calf thymus DNA (58%), E. coli (DNA (50%) and M. lysodeikticus DNA (28%) these DNA samples were used in these experiments... Fig. 22. Effect of tilorone on the Tm with DNA preparations from various sources of different base compositions. Tm values were obtained at a molar ratio of drug to DNA-P of 0,21. The Tm of DNA (5 x 10 SM) with and without tilorone was determined in a medium of 0.01 M Tris-HCl (pH 7.0). AT-content of B. cereus DNA (65%), calf thymus DNA (58%), E. coli (DNA (50%) and M. lysodeikticus DNA (28%) these DNA samples were used in these experiments...
Relative amplitudes of ti, T2, and T3 for actinomycin D dissociation from calf thymus DNA. [Pg.279]

The structure of the major photoproduct of irradiation of the dinucleotide d(TpA) and TA containing oligonucleotides has been re-characterised. 2 -Deoxyoxoanosine (218) has been isolated from calf thymus DNA after treatment with the mutagens nitrous acid or nitric oxide. ... [Pg.226]

How might you deduce from a Cgt plot that the gene density in higher eukaryotes, e.g., from calf thymus DNA as in Prob. 7.17, is much lower than in E. coin... [Pg.233]

Stein, H. and Hausen, P. (1969) Enzyme from calf thymus degrading the RNA moiety of DNA-RNA hybrids effect on DNA dependent RNA polymerase. Science, 166, 393—395. [Pg.756]

Ferro AM, Olivera BM. Poly (ADP-ribosylation) of DNA topoisomerase I from calf thymus. J Biol Chem 1984 259 547-554. [Pg.233]


See other pages where DNA from calf thymus is mentioned: [Pg.15]    [Pg.382]    [Pg.16]    [Pg.146]    [Pg.161]    [Pg.296]    [Pg.233]    [Pg.111]    [Pg.120]    [Pg.117]    [Pg.15]    [Pg.382]    [Pg.16]    [Pg.146]    [Pg.161]    [Pg.296]    [Pg.233]    [Pg.111]    [Pg.120]    [Pg.117]    [Pg.243]    [Pg.1491]    [Pg.277]    [Pg.168]    [Pg.139]    [Pg.141]    [Pg.299]    [Pg.127]    [Pg.6]    [Pg.322]    [Pg.126]    [Pg.239]    [Pg.85]    [Pg.269]    [Pg.270]    [Pg.279]    [Pg.6]    [Pg.557]    [Pg.293]    [Pg.199]    [Pg.250]    [Pg.170]    [Pg.412]   
See also in sourсe #XX -- [ Pg.26 ]




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