Migration Inhibition Test

The migration inhibition test was applied in our laboratory for diagnosis of allergic reactions to drugs, for two main reasons  

1) The test procedure is simple, inexpensive, and the result is obtained the following day, which represents significant advantages when compared with the lymphocyte transformation test. 

2) As shown by Rocklin et al. (1974), B-lymphocytes as well as T-cells showed the capacity to produce the inhibitory factor when put in contact with mitogens or specific antigens. Therefore, lymphokine production could result from both humoral imune reactions and cell-mediated reactions. 

Experiments were performed with several drugs however, 15 cases of penicillin allergy were thoroughly studied according to the type of immune reaction. The result shown in Table 12 demonstrates a good correlation between the leukocyte mi- 

Tabk 12. MIF production by leucocytes from 15 patients allergic to penicillin. Comparison with the lymphocyte transformation test (LTT) and skin tests 

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For in vitro testing of nickel contact sensitivity, the lymphocyte transformation test and leucocyte migration inhibition test have been used [370]. 

Lymphoblast transformation test, the leukocyte migration inhibition test with specific allergen (Kaczmarski et al. 1997). 

The leukocyte migration inhibition test has been suggested to be a reliable test in cow s milk allergy but has been shown to be quite unspecific in other conditions (Kaczmarski et al. 1997). 

Brenner S, Halevy S, Livni E, Schewach-Millet M, Sandbank M, Wolf R. Macrophage migration inhibition test in patients with drug-induced pemphigus. Isr J Med Sci 1993 29(l) 44-6. 

For these reasons, lymphocyte transformation and leukocyte migration inhibition tests have been widely used to investigate type IV allergic drug reactions. 

Jordan WP, Higgins M, Dvorak J (1975) Allergic contact dermatitis to all-transretinoic acid epicutaneous and leukocyte migration inhibition testing. Contact Dermatitis 1 306-310... 

Positive macrophage migration inhibition tests in patients with penicillin-induced exanthema (not absolute evidence of delayed hypersensitivity immune complexes can also induce migration inhibition). 

There are no reports in the literature concerning results obtained with in vitro lymphocyte transformation or leukocyte migration inhibition tests in patients with suspected allergy to the lincomycins. 

Chyrek-Borowska S, Kama T, Obrzut D (1973) Leukocyte migration inhibition tests in investigations of allergy to treptomycin. Pol Tyg Lek 28 1536-1540 Coles RB, Philips J, Nuttall JB (1967) Anaphylactoid reaction to demethyl-chlortetracycline (Ledermycin). Br Med J 2 313-314... 

Warrington and Olivier (1979) examined the release of lymphotoxin from the lymphocytes of the peripheral blood in patients with hepatitis after isoniazid. In five of six cases there was support of release of lymphotoxin after stimulation of these lymphocytes with isoniazid or isonicotinic acid conjugated with human serum albumin. Baker et al. (1974) had positive results from the macrophage migration inhibition test in only two of six patients after clinical hypersensitivity to isoniazid. 

James P (1978) Vitamin E and malignant hyperthemia. Br Med J 1 1345 James P 0979) Vitamin E and malignant hyperthermia. Br Med J 1 200 Jirasek L, Schwank R (1965) Berufskontaktekzem durch Vitamin K. Hautarzt 18 353-355 Jordan WP, Higgins M, Dvorak J (1975) Allergic contact dermatitis to all-trans-retinoic acid epicutaneous and leukocyte migration inhibition testing. Contact Dermatitis 1 306-310... 

The two most common in vitro test procedures used are lymphocyte proliferation and cell migration inhibition tests. The basic theory behind both of these is that T-cells have receptors on their surface which will each respond to a specific antigen. In the course of the response, soluble substances (cytokines or lymphokines), principally blastogenic factor and migration inhibition factor, which act on other cells, including other T-cells, are produced and released. 

Nicole A, Fasel-Felley J, Perrisond D, Frei P C 1985 Influence of palmitoyl-3-catechin and hep-tyl-3-catechin on leukocyte migration inhibition test carried out in the presence of PPD and hepatitis B surface antigen (HBsAg). Int J Immunopharmacol 7 87-92... 

WilUams WR, Jones-WiUiams W. Comparison of lymphocyte transformation and macrophage migration inhibition tests in the detection of beryllium hypersensitivity. J Clin Pathol 1982 35(6) 684-687. 

Two cases of contact allergic reactions to locally applied retinoic acid were reported by Jordan et al. (10 ). Two volunteers were found to be allergic to retinoic acid at the beginning of a predictive test designed to compare the skin irritation potential of various acne formulations. The patch testing procedures reported by the authors eliminate the possibility of a response due to irritation and the leukocyte migration inhibition test supports the existence of a true cell-mediated response. 

PGE2 2.75X 10- M solution migration inhibition test and parainfluenza type 1 virus in lymphocytes showing vigorous response to virus and enhanced In those... 

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