Mass spectrometry CE/MS

As in HPLC, the coupling of MS detection with CE has provided an excellent opportunity for more selective analysis, but the much reduced flow rates, small injection volumes, limitations in the types of buffers used [since electrospray ionization (ESI) is used in capillary electrophoresis/mass spectrometry (CE/MS)], and need to... 

MOR were comparable (10 ng mL4), whereas those for DHC and EMOR were about fourfold lower. Furthermore, glucuronides were shown to react like the corresponding free opioids. Validation with real urine samples was performed with identification of the peaks by capillary electrophoresis-ion-trap mass spectrometry (CE-MS) after solid-phase extraction. 

Capillary electrochromatography-mass spectrometry (CE-MS), 4 641 Capillary electrodes, 14 27 Capillary electrophoresis (CE), 4 602-603, 631-633 6 385 9 751-752 antibody based columns with, 6 402 chiral additives, 6 77-79 applications, 4 641 basic principles, 4 606-609 detectors, 4 634-635 for DNA analysis, 4 636-637 flow profiles generated, 4 608 instrumentation, 4 633 as microfluidic assay technique,... 

The hyphenation of CE with techniques allowing structural determination of impurities is the object of Chapter 18 Coupling CE and Microchip-based Devices and Mass Spectrometry of this book, but the potential of CE coupled to mass spectrometry (CE/MS) in impurity profiling has also been discussed elsewhere. ... 

Recent developments in capillary electrophoresis coupled to mass spectrometry (CE—MS) are reviewed from over 400 references covering the significant challenges, issues, and results obtained with CE—MS analyses. 

The high-resolution power of capillary electrophoresis-mass spectrometry (CE-MS) makes it a powerful technique for the separation and analysis of charged metabolites however, only a few metabolomics applications have been published to date. Polypeptide fraction isolated from urine or plasma was analyzed by CE-MS and used to discriminate between coronary artery... 

As an instrumental approach to conventional electrophoresis, capillary electrophoresis offers the capability of on-line detection, micropreparative operation and automation (6,8,45-47). In addition, the in tandem connection of capillary electrophoresis to other spectroscopy techniques, such as mass spectrometry, provides high information content on many components of the simple or complex peptide under study. For example, it has been possible to separate and characterize various dynorphins by capillary electrophoresis-mass spectrometry (33). Therefore, the combination of CE-mass spectrometry (CE-MS) provides a valuable analytical tool useful for the fast identification and structural characterization of peptides. Recently, it has been demonstrated that the use of atmospheric pressure ionization using Ion Spray Liquid Chromatography/ Mass Spectrometry is well suited for CE/MS (48). This approach to CE/MS provides a very effective and straightforward method which allow the feasibility of obtaining CE/MS data for peptides from actual biological extracts, i.e., analysis of neuropeptides from equine cerebral spinal fluid (33). 

Capillary electrophoresis mass spectrometry (CE-MS) Direct coupling of capillary electrophoresis (CE) and MS, where the mass spectrometer serves as the detector. Biomolecules are separated through the narrow capillary of the CE system and then interface with the MS for subsequent analysis. 

Surprisingly, not very many methods using the capillary electrophoresis-mass spectrometry (CE-MS) combination (mainly electrospray ionization) are in use in the area of forensic drug analysis. The first application of CE-MS for forensic purposes was described by Johansson et al. in 1991 [10] for the analysis of sulfonamides and benzodiazepines in urine. The main problem faced in this case is the need of improving the concentration sensitivity of the CE-MS combination. In order to improve the concentration of ana-... 

Capillary electrophoresis/mass spectrometry (CE/MS) is a useful tool for bioanalysis because it combines a highly efficient separation technique with a sensitive and selective detection system. Because CE offers excellent mass sensitivity but presents challenges when concentration sensitivity is needed, more sensitive detection systems are required. As an example of this, preliminary results for... 

In another application, Sturm et al. (44) evaluated CE-mass spectrometric (capillary electrophoresis-mass spectrometry [CE-MS]) data from different Corydalis species, that is, Corydalis cava from two different regions (CM and CB), Corydalis pumila (PU), Corydalis intermedia (IN), and Corydalis solida from two different regions (SG and SE). Exploring the CE-MS data with PCA succeeded in distinguishing the six Corydalis species samples. In this example, only a comprehensive list of peak areas of analytes was chosen as initial data... 

In recent years, the coupling of capillary electrophoresis to mass spectrometry (CE-MS) has moved at an extraordinary pace, expanding out of the realm of a few and simple analytical chemistry applications into the development... 

Capillary electrophoresis-mass spectrometry (CE-MS) combines the separating power of CE with the uniquely powerful detection capabilities of MS. This hyphenated system enables the analyst to separate, identify and quantify components in a mixture. As with GC-MS and LC-MS instruments, there is a variety of mass spectrometric ionisation techniques and analysers available. 

Capillary-electromigration separation techniques are coupled to mass spectrometry (CE-MS) by interfaces based on proven LC-MS designs [41,49,80-85]. In early... 

The analyses of egg proteins by CZE, CIEF, or MEKC were reviewed by Recio et al. In addition, an interesting application of CE coupled to mass spectrometry (CE-MS) was the detection of lysozyme from chicken and turkey egg white. Since one of the problems of protein separations was the possible interaction with the capillary wall, a polymer coating compatible with CE-MS was developed, and the usefulness of this method was shown by the analysis of a minced meat extract containing chicken egg white as adulterant (Table 30.8). ... 

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