Microchip-based devices

XVIII. COUPLING CE AND MICROCHIP-BASED DEVICES WITH MASS SPECTROMETRY... 

The hyphenation of CE with techniques allowing structural determination of impurities is the object of Chapter 18 Coupling CE and Microchip-based Devices and Mass Spectrometry of this book, but the potential of CE coupled to mass spectrometry (CE/MS) in impurity profiling has also been discussed elsewhere. ... 

Cheng, J., Waters, L.C., Fortina, P., Hvichia, G., Jacobson, S.C., Ramsey, J.M., Kricka, L.J., Wilding, P., Degenerate oligonucleotide primed-polymerase chain reaction and capillary electrophoretic analysis of human DNA on microchip-based devices. Anal. Biochem. 1998, 257(2), 101-106. 

J. Cheng, R. Fortina, S. Sorrey, L.J. Kricka and P. Wilding, Microchip-based devices for molecular diagnosis of genetic diseases, Mol. Diagn., 1 (1996) 183-200. 

One of the earliest efforts of qualitative measurement of a protein (human serum albumin) in a microchip-based device was based on bead agglutination in a microchamber (approximately lOjaL). Subsequently, several quantitative immunoassays have been performed using microchip electrophoretic systems that permit separation and quantitation of free- and bound-labeled antigens in competitive assays (see Chapter 5). Most are carried out in channels micro-machined into fused silica substrates. Early work on quantitative assays achieved measurement of cortisol in serum.The assay used cortisol labeled with fluorescein and an argon laser detector at 488 nm and required only 80 pL of a 40x dilution of serum as the sample. Other capillary electrophoresis-based assays for a variety of antibodies have also been developed that include immunoglobulins (IgG, IgA, and IgM), antibovine serum albumin, and antiestradiol. ... 

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