Capillary electrophoresis coupling

In E. Coli bacterial lysates, the proteome (i.e., the full array of proteins produced) was analyzed by isoelectric focusing and mass spectrometry.97 A comparison of capillary electrophoretic separation and slab gel separation of a recombinant monoclonal antibody demonstrated that the precision, robustness, speed, and ease-of-use of CE were superior.98 Seventy-five proteins from the yeast ribosome were analyzed and identified by capillary electrophoresis coupled with MS/MS tandem mass spectrometry.99 Heavy-chain C-terminal variants of the anti-tumor necrosis factor antibody DE7 have been separated on capillary isoelectric focusing.100 Isoforms differing by about 0.1 pi units represented antibodies with 0,1 or 2 C-terminal lysines. 

Liu, Y., Sweedler, J.V. (1996). Two-dimensional separations capillary electrophoresis coupled to channel gel electrophoresis. Anal. Chem. 68(22), 3928-3933. 

Bergstrom, S.K., Samskog, J., Markides, K.E. (2003). Development of a poly(dimethylsilox-ane) interface for on-line capillary column liquid chromatography-capillary electrophoresis coupled to sheathless electrospray ionization time-of-flight mass spectrometry. Anal. Chem. 75, 5461-5467. 

Huck WC, Stecher G, Scherz H and Bonn G. 2005. Analysis of drugs, natural and bioactive compounds containing phenolic groups by capillary electrophoresis coupled to mass spectrometry. Electrophoresis 26(7-8) 1319-1333. 

K. Arlt, S. Brandt, and J. Kehr, Amino acid analysis in five pooled single plant cell samples using capillary electrophoresis coupled to laser induced fluorescence detection. J. Chromatogr. A 926, 319 325 (2001). 

Capillary electrophoresis coupled with a laser-induced fluorescence (LIF) detector has also been applied for the analysis of copper chlorophyll in olive oils. Samples were... 

L. Arce, A. Rios and M. Valcarcel, Determination of anti-carcinogenic polyphenols present in green tea using capillary electrophoresis coupled to a flow injection system. J. Chromatogr.A 823 (1998) 113-120. 

Varesio E, Rudaz S, Krause KH, Veuthey JL. 2002. Nanoscale liquid chromatography and capillary electrophoresis coupled to electrospray mass spectrometry for the detection of amyloid-beta peptide related to Alzheimer s disease. 

Sanz-Nebot, V., Benavente, R., Balaguer, E., and Barbosa, J. (2003). Capillary electrophoresis coupled to time of flight-mass spectrometry of therapeutic peptide hormones. Electrophoresis 24, 883-891. 

Recent developments in capillary electrophoresis coupled to mass spectrometry (CE—MS) are reviewed from over 400 references covering the significant challenges, issues, and results obtained with CE—MS analyses. 

Huck, C. W., Bakry, R., Huber, L. A., and Bonn, G. K. (2006). Progress in capillary electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Electrophoresis 27, 2063—2074. 

Chakel, J. A., Erno, J., Hancock, W. S., and Swedberg, S. A. (1997). Analysis of recombinant DNA-derived glycoproteins via high-performance capillary electrophoresis coupled with off-line matrix-assisted laser desorption ionization time-of-fiight mass spectrometry. /. Chromatogr. B 689, 215—220. 

Fliser, D., Wittke, S., and Mischak, H. (2005). Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes. Electrophoresis 26, 2708 — 2716. 

Hamdan, M. (1997). Pharmaceutical applications of liquid chromatography, capillary electrophoresis coupled to mass spectrometry. Process Control Qual. 10, 113 — 127. 

Sheppard, R. L., Tong, X., Cai, J., and Henion, J. D. (1995). Chiral separation and detection of terbutaline and ephedrine by capillary electrophoresis coupled with ion spray mass spectrometry. Anal. Chem. 67, 2054-2058. 

Geiser, L., Cherkaoui, S., and Veuthey, J. L. (2002). Potential of formamide and N-methylformamide in nonaqueous capillary electrophoresis coupled to electrospray ionization mass spectrometry. Application to the analysis of P-blockers. /. Chromatogr. A 979, 389—398. 

Mass spectrometry (MS) is probably a famhiar tool to chemistry and biology students as a technique commonly used to measure the molecular mass of a sample. Often, MS is used in tandem with other techniques for chromatic separation of the sample before mass measurement. Some common hyphenated techniques include HPLC-MS, high-pressure liquid chromatography coupled to MS GC-MS, gas chromatography coupled to MS or CE-MS, capillary electrophoresis coupled to MS. 

Separation and detection techniques for antibacterials in food mainly focus on the use of LC coupled to MS or tandem MS. Nevertheless, recent studies have suggested capillary electrophoresis coupled to laser-induced fluorescence (LIE) as a way of improving sensitivity [49], HRLC coupled to microTOF-ESI-MS as a highly selective, sensitive, and quick screening method for 100 veterinary drugs in fish, meat, and egg samples [195], and nanoscale LC coupled to UV or ion trap MS, with LODs in the range 0.01-0.51 pg/L (nanoLC-MS) and the possibility that even lower limits could be achieved by using triple quadrupole MS [59]. 

Wang, J., G. Chen, M. Chatrathi, K. Shin, and A. Fujishima. Microchip capillary electrophoresis coupled with a boron-doped diamond electrode-based electrochemical detector. Anal. Chem. 75, 935-939 (2003). 

NA Guzman. Determination of immunoreactive gonadotropin-releasing hormone in serum and urine by on-line immunoaffinity capillary electrophoresis coupled to mass spectrometry. J Chromatogr B 749 197—213, 2000. 

N. E. Hebert, W.G. Ruhr and S.A. Brazill, Microchip capillary electrophoresis coupled to sinusoidal voltammetry for the detection of native carbohydrates, Electrophoresis, 23 (2002) 3750-3759. 

Bianco, G., Schmitt-Kopplin, R, De Benedetto, G., Kettrup, A., and Cataldi, T. R. (2002). Determination of glycoalkaloids and relative aglycones by non-aqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry. Electrophoresis 23,2904—2912. 

Liu, Y., Lopez-Avila, V, Zhu, J.J., Wiederin, D.R. and Beckert, W.F. (1995) Capillary electrophoresis coupled on-line with inductively coupled plasma-mass spectrometry for elemental speciation. Anal Chem., 67, 2020-2025. 

Michalke, B., O. Schramel, and A. Kettrup. 1999. Capillary electrophoresis coupled to inductively coupled plasma mass spectrometry (CE/ICP-MS) and to electrospray ionization mass spectrometry (CE/ ESI-MS) An approach for maximum species information in speciation of selenium. Fresenius J. Anal. Chem. 363 456—459. 

C.E. Kientz, E.W.J. Hooijschuur and U.A.Th. Brinkman, Capillary electrophoresis coupled online with flame photometric detection determination of alkylphosphonic acids, J. Microcol. Sep., 9, 253-259 (1997). 

Wittke S, Mischak H, Walden M, Kolch W, Radler T, Wiedemann K (2005) Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled to mass spectrometry Towards new diagnosdc and dierapeudc approaches. Electrophoresis 26 147 1487. 

L. A. Holland and S. M. Lunte, Capillary electrophoresis coupled to electrochemical detection A review of recent advances. Ana/. Commun. 35 1H-4H (1998). 

Capillary Electrophoresis Coupled to Mass Spectrometry for Chiral Drugs Analysis"... 

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