Microfluidic assays

Capillary electrochromatography-mass spectrometry (CE-MS), 4 641 Capillary electrodes, 14 27 Capillary electrophoresis (CE), 4 602-603, 631-633 6 385 9 751-752 antibody based columns with, 6 402 chiral additives, 6 77-79 applications, 4 641 basic principles, 4 606-609 detectors, 4 634-635 for DNA analysis, 4 636-637 flow profiles generated, 4 608 instrumentation, 4 633 as microfluidic assay technique,... 

See also Gas chromatography Capillary gel electrophoresis (CGE), in microfluidic assays, 26 971 Capillary hydrodynamic flow (CHDF) techniques, 16 291 20 381 Capillary microfiltration/ultrafiltration (MF/UF) technology, 26 83-84 Capillary number, 11 746 Capillary optics, 26 438 Capillary pumped loops (CPLs),... 

Two-dimensional (2D) separation systems in microfluidic assays, 26 970 Two-dimensional (2D) transistor strustures, in scaling to deep submicron dimensions, 22 256 Two-dimensional data searches, 6 6-8 Two-dimensional electrode flow cells, 9 664-665... 

Pommereau, A., Pap, E., and Kannt, A., Two simple and generic antibody-independent kinase assays comparison of a bioluminescent and a microfluidic assay format, /. Biomol. Screen., 9, 409, 2004. 

IMMUSOFT A PROGRAM FOR COMPUTER-DRIVEN MICROFLUIDIC ASSAYS... 

The pressure of cost constraints and throughput will continue to drive the need for assay miniaturisation. Plate-based assay technologies will continue to be the dominant assay format, but non-plate screening technologies may come to have more impact in future years. It is also possible that microfluidic assays and chip-based assay technologies will lead the miniaturisation of HTS assays into new dimensions. 

Besides the microfluidic assay that was described in Section 3.1 of this chapter, we would like to discuss another example out of many others, where we feel that this strategy of nesting different approaches for miniaturization and parallelization in a clever way will lead or has led to substantial improvements. 

Fluorescence Off-chip microfluidic assay The electrophoretic seperation and detection of substrate and product take place in the channels of the chip Medium... 

Hadd AG, Jacobson SC, Ramsey JM. Microfluidic Assays of Acetylcholinesterase Inhibitors. Anal Chem 1999 71 5206-12. 

Mao, H., Cremer, P. S., and Manson, M. D. (2003) A sensitive, versatile microfluidic assay for bacterial chemotaxis. Proc. Natl. Acad. Sci. U5A100,5449-5454. 

High pressure-rated channels allow a smaller construction of microfluidic assays at the same throughput, which arises due to higher possible flow rates. Such devices are of interest for the cost-effective integration of CMOS chips to microfluidic circuits [45]. 

Cell migration microfluidic assay Microfluidic assay for cell migration... 

Christ KV et al (2010) Measurement of single-cell adhesion strength using a microfluidic assay. Biomed Microdevices 12(3) 443 55... 

Colace T, Fogarty PF, Panckeri KA, Li R, Diamond SL. Microfluidic assay of hemophihc blood clotting distinct deficits in platelet and fibrin deposition at low factor levels. J Thromb Haemost 2014 12 147-58. 

Aspartate transaminase (AST) or the equivalent serum glutamic oxaloacetic transaminase (SGOT) and alanine transaminase (ALT) or the equivalent serum glutamic pyruvic transaminase (SGPT) are useful indicators of liver conditions such as alcoholic liver disease (Cohen and Kaplan, 1979). A multiplexed, paper-based microfluidic assay designed for quick, semiquantitative measurement of AST and ALT in a hn-ger-stick specimen has been tested clinically (Pollock et al., 2012). Such paper-based techniques have also been supplemented by optical detection (Swanson et al., 2015). Microfluidic channels combined with electrochemical sensors have also used to measure ALT and AST (Song et al., 2009). 

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