Liquid chromatography/ultraviolet/mass

Zhao, Y. et al., High-throughput logP measurement using parallel liquid chromatography/ ultraviolet mass spectrometry and sample-pooling, Rapid Commun. Mass Spectrom., 16, 1548, 2002. [Pg.57]

This LC-MS experimental approaches used in NEF metabolite identification are also routinely employed for in vitro metabolism comparisons across species, in which liver microsomes or hepatocytes from humans and animal species are used. In addition, liver microsomal incubations followed by metabolite identification and quantitative estimation using liquid chromatography-ultraviolet/ mass spectrometry (LC-UV/MS) is an approach commonly taken to determine metabolic soft spots, where a major metabolic reaction takes place (Table 6.10). Use of UV detection allows for quantitative analysis of major metabolites in the absence of chemical standards, with the assumption that the metabolic reaction did not disturb the molecule s UV chromophore. [Pg.159]

The method for chloroacetanilide soil metabolites in water determines concentrations of ethanesulfonic acid (ESA) and oxanilic acid (OXA) metabolites of alachlor, acetochlor, and metolachlor in surface water and groundwater samples by direct aqueous injection LC/MS/MS. After injection, compounds are separated by reversed-phase HPLC and introduced into the mass spectrometer with a TurboIonSpray atmospheric pressure ionization (API) interface. Using direct aqueous injection without prior SPE and/or concentration minimizes losses and greatly simplifies the analytical procedure. Standard addition experiments can be used to check for matrix effects. With multiple-reaction monitoring in the negative electrospray ionization mode, LC/MS/MS provides superior specificity and sensitivity compared with conventional liquid chromatography/mass spectrometry (LC/MS) or liquid chromatography/ultraviolet detection (LC/UV), and the need for a confirmatory method is eliminated. In summary,... [Pg.349]

Fang, L. et al. 2003. Parallel high-throughput accurate mass measurement using a nine-channel multiplexed electrospray liquid chromatography ultraviolet time-of-flight mass spectrometry system. Rapid Commun. Mass Spectrom. 17 1425. [Pg.243]

GC = gas chromatography HC1 = hydrochloric acid HPLC = high-performance liquid chromatography MS = mass spectrometry Na2S04 = sodium sulfate NR = not reported UV = ultraviolet detector... [Pg.108]

C.W. Huck, M.R. Buchmeiser and G.K. Bonn, Fast analysis of flavonoids in plant extracts by liquid chromatography-ultraviolet absorbance detection on poly(carboxylic acid)coated silica and electrospray ionization tandem mass spectrometric detection. J. Chromatogr.A 943 (2002) 33-38. [Pg.357]

Cheng Y.F. Lu Z. Neuc U. Ultrafast liquid chromatography/ultraviolet and liquid chromatography/tandem mass spectrometric analysis. Rapid Communications in Mass Spectrometry, 2001, 15, 141-151. [Pg.66]

Pihlainen, K. and Kostiainen, R., Effect of the eluent on enantiomer separation of controlled drugs by liquid chromatography-ultraviolet absorbance detection-electrospray ionisation tandem mass spectrometry using vancomycin and native fi-cyclodextrin chiral stationary phases, J. Chromatogr. A, 1033, 91, 2004. [Pg.168]

Radioactivity (17) Isotope dilution-radioimmunoassay (19) Ultraviolet (17) Thin-layer chromatography (16) Gas-liquid chromatography (16) Mass spectrometry (16) HPLC (20)... [Pg.594]

GC, gas chromatography HPLC, high-performance liquid chromatography MS, mass spectroscopy AA, atomic absorption GFAA, graphite furnace atomic absorption ICP, inductively coupled plasma UV-VIS, ultraviolet-visible molecular absorption spectroscopy IC, ion chromatography. [Pg.4]

Awidi A et al (2010) Determination of imatinib plasma levels in patients with chronic myeloid leukemia by high performance liquid chromatography-ultraviolet detection and liquid chromatography-tandem mass spectrometry methods comparison. Leuk Res 34 714-717... [Pg.243]

CLP = contract laboratory program EC = electron capture detection FSCC-MS/DS = fused silica capillary, mass spectrometry/data system GC = gas chromatography HPLC-UV = high-resolution liquid chromatography - ultraviolet detection 2-NP = 2-nitrophenol 4-NP = 4-nitrophenol... [Pg.85]

D. Sanchez-Rodas, A. Geiszinger, J. L. Gomez-Ariza, K. A. Francesconi, Determination of an arsenosugar in oyster extracts by liquid chromatography electrospray mass spectrometry and liquid chromatography-ultraviolet photooxidation-hydride generation atomic fluorescence spectroscopy, Analyst, 127 (2002), 60-65. [Pg.593]

Isolation of Processes To minimize cross-contamination and microbiological contamination, the manufacturer may develop special procedures for the isolation of processes. The level of facilities isolation depends on the types of products to be manufactured. For instance, steroids and sulfas require more isolation than over-the-counter (OTC) oral products [6], To minimize exposure of personnel to drug aerosols and loss of product, a sealed pressure vessel must be used to compound aerosol suspensions and emulsions [21], An example of cross-contamination with steroids was the controversial case of a topical drug manufactured for the treatment of skin diseases. Fligh-performance liquid chromatography/ultraviolet and mass spectrometry (FIPLC/UV, FIPLC/MS) techniques were used by the FDA for the detection of clobetasol propionate, a class 1 superpotent steroid, as an undeclared steroid in zinc pyrithione formulations. The product was forbidden and a warning was widely published [22],... [Pg.320]

Determination of sulphonamides by liquid-chromatography, ultraviolet diode-array detection and ion-spray tandem mass-spectrometry with application to cultured salmon flesh. J. Chromatogr. 1991, 558, 155-173. [Pg.551]

Is the method specific and stability indicating as shown by analysis of samples subjected to stressed stability studies (pH, light, heat, oxidation) Normally, specificity is determined through peak purity using ultraviolet (UV) diode array or liquid chromatography (LC)/mass spectrometry (MS) analysis. In methods for analysis of drug products, placebo formulations (and stressed placebos) must yield blank chromatographic baselines. The development... [Pg.424]