High-performance liquid chromatography with ultraviolet-mass spectrometry

Quilliam MA. Sim PG. 1988. Determination of polycyclic aromatic compounds by high-performance liquid chromatography with simultaneous mass spectrometry and ultraviolet diode array detection. J Chromatogr Sci 26 160-167. 

Lin C-H, RH Lerch, EM Thurmkan, HE Garrett, ME George (2002) Determination of isoxaflutole (Balance) and its metabolites in water using solid-phase extraction followed by high-performance liquid chromatography with ultraviolet or mass spectrometry. J Agric Eood Chem 50 5816-5824. 

Koupai-Abyazani, M.R. Esaw, B. Laviolette, B. Etodolac in equine urine and serum determination by high-performance liquid chromatography with ultraviolet detection, confirmation and metabolite identification by atmospheric pressure ionization mass spectrometry. J. Anal. Toxicol. 1999, 23 (3), 200-209. 

Initially, simple methods such as ultraviolet-visible (UV-Vis), fluorescence or infrared (IR) spectroscopy were proposed in order to estimate the total amount of antioxidants in various food samples. However, coupled methods such as gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography with ultraviolet-visible (HPLC-UV-Vis) or nuclear magnetic resonance detector (HPLC-NMR) and liquid chromatography-mass spectrometry (LC-MS) are employed more to quantify individual tocols or carotens from various corn-based food samples. In this chapter all these methods of analysis will be briefly described. 

S. Hakkinen and S. Auriola, High performance liquid chromatography with electrospray ioniza tion mass spectrometry and diode array ultraviolet detection in the identification of flavonol aglycones and glycosides in berries, J. Chromatogr. A, 829, 91 100 (1998). 

Baud-Camus F, Marquet P, Soursac M et at. (2001) Determination of N-acetylation phenotype using caffeine as a metabolic probe and high-performance liquid chromatography with either ultraviolet detection or electrospray mass spectrometry. J Chromatogr B 760 55-63... 

Reepmeyer, J. C., Revelle, L. K., and Vidavsky, I. (1998), Detection of clobetasol propionate as an undeclared steroid in zinc pyrithione formulations by high-performance liquid chromatography with rapid-scanning ultraviolet spectroscopy and mass spectrometry. Journal of Chromatography A, Vol. 828, Number 1,18 December 1998, pp. 239-246(8). 

SILVA, O, GOMES, E.T., WOLFENDER, J.L., MARSTON, A., HOSTETTMANN, K., Application of high performance liquid chromatography coupled with ultraviolet spectroscopy and electrospray mass spectrometry to the characterisation of ellagitannins from Terminalia macroptera roots, Pharm. Res., 2000,17, 1396-1401. 

Lanina et al. 1992 Oishi 1990). These methods include gas chromatography (GC) combined with mass spectrometry (MS) and high-performance liquid chromatography (HPLC) combined with an ultraviolet detector (UV). No comparisons can be made between methods since no data were given regarding sensitivity, recovery, or precision. 

Awidi A et al (2010) Determination of imatinib plasma levels in patients with chronic myeloid leukemia by high performance liquid chromatography-ultraviolet detection and liquid chromatography-tandem mass spectrometry methods comparison. Leuk Res 34 714-717... 

As discussed in Chapter 6, the primary method of analyzing benzene in body fluids and tissues is gas chromatography (GC) in conjunction with either mass spectrometry (MS), photoionization detection (PID), or flame ionization detection (FID). For detection of benzene metabolites, both GC/FID and high-performance liquid chromatography (FIPLC) with ultraviolet detection (UV) have been used. A recent article describes the development of simple and sensitive methods for determination of blood and urinary benzene levels using gas chromatography headspace method (Kok and Ong 1994). 

Modern tHcy methods include enzyme immunoassays and chromatographic-based methods. In practice, immunoassays " are most often used for routine purposes (e.g., fluorescence polarization immunoassay IFPIA] as run on Abbott s IMx and AxSYM platforms) Chromatographic assays include amino acid analysis high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection ° " capillary electrophoresis with fluorescence detection gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with tandem MS (MS-MS). 

EDCs in the environment are often analyzed using GC or LC based instrumental techniques. GC coupled with an electron capture detector (BCD), a nitrogen-phosphorus detector (NPD), or mass spectrometry (MS) has been the preferred method due to its excellent sensitivity and separation capability on a capillary column. High performance liquid chromatography (HPLC) with various detectors such as ultraviolet detection (UV), fluorescence detection (FLD), MS, and more recently tandem MS (MS/MS) has also been used for analysis of some EDCs, especially for the polar compounds. Analytical techniques for each class of EDCs will be discussed in the following section. 

Silva O, Gomes ET, Wolfender JL, Marston A, Hostettmann K. (2000).Application of High Performance Liquid Chromatography coupled with Ultraviolet spectroscopy and Electrospray Mass Spectrometry to the characterisation of Ellagitannins from Terminalia macroptera Roots. Pharmaceutical Research, Vol. 17, pp. 1396-1401... 

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