Hydride generation atomic fluorescence

Arsenic is both toxic and cai cinogenic element. It is necessary to have a fast, reliable and accurate method for determination of ai senic in water. The hydride-generation atomic fluorescence spectrometry (HG AFS) is one of the simple and sensitive techniques for the determination of this element in various types of waters. 

Total dissolved Fe and Mn were analyzed directly by flame atomic absorption spectrometry (AAS). As was measured by AAS with hydride generation (HG-FIAS). Total dissolved Se concentrations were determined by hydride-generation atomic fluorescence spectrometry (Chen etal., 2005). 

Compare atomic absorption (both flame and graphite furnace), ICP, flame photometry, cold vapor mercury, hydride generation, atomic fluorescence, and spark emission in terms of ... 

B.6 Speciation of Arsenic Compounds by Ion-Exchange High-Performance Liquid Chromatography with Hydride Generation Atomic Fluorescence Detection. 

The instrumental conditions for the hydride generation atomic fluorescence instrument are given in Table B.2. A diagram of the instrumental set-up is shown in Fig. B.l. 

Table B.2 Instrumental conditions for hydride generation atomic fluorescence spectrometry.

J. Gomez-Ariza, M.-A. Caro-de-la-Torre, I. Giraldez and E. Morales, Speciation analysis of selenium compounds in yeasts using pressurized liquid extraction and liquid chromatography-microwave-assisted digestion-hydride generation-atomic fluorescence spectrometry. Anal. Chim. Acta, 524(1-2), 2004, 305-314. 

C. Moscoso-Perez, J. Moreda-Pineiro, P. Lopez-Mahia, S. Muniategui-Lorenzo, E. Fernandez-Fernandez and D. Prada-Rodriguez, As, Bi, Se(IV) and Te(IV) determination in acid extracts of raw materials and byproducts from coal-fired power plants by hydride generation-atomic fluorescence spectrometry. At. Spectrosc., 25(5), 2004, 211-216. 

Pitts, L., A. Fisher, P. Worsfold, and S.J. Hill. 1995. Selenium speciation using high-performance liquid chromatography-hydride generation atomic fluorescence with on-line microwave reduction. J. Anal. At. Spectrom. 10 519-520. 

E. Moreno, C. Camara, W. T. Cores, D. W. Bryce, P. Stockwell, Arsenic speciation in beverages by direct injection-ion chromatography-hydride generation atomic fluorescence spectrometry, J. Autom. Met. Manag. Chem., 22 (2000), 33-39. 

M. A. Suner, V. Devesa, I. Rivas, D. Velez, R. Montoro, Speciation of cationic arsenic species in seafood by coupling liquid chromatography with hydride generation atomic fluorescence detection, J. Anal. Atom. Spectrom., 15 (2000), 1501-1508. 

D. Sanchez-Rodas, A. Geiszinger, J. L. Gomez-Ariza, K. A. Francesconi, Determination of an arsenosugar in oyster extracts by liquid chromatography electrospray mass spectrometry and liquid chromatography-ultraviolet photooxidation-hydride generation atomic fluorescence spectroscopy, Analyst, 127 (2002), 60-65. 

C. Chen, J. Zhao, P. Zhang, Z. Chai, Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection, Anal. Bioanal. Chem., 372 (2002), 426-430. 

D. Wallschlager, N. S. Bloom, Determination of selenite, selenate and selenocyanate in waters by ion chromatography-hydride generation-atomic fluorescence spectroscopy (IC-HG-AFS), J. Anal. Atom. Spectrom., 16 (2001), 1322-1328. 

Vinas, P., Lopez-Garcia, I., Merino-Merofio, B., Campillo, N., Hemandez-Cordoba, M. Determination of selenium species in infant formulas and dietetic supplements using liquid chromatography-hydride generation atomic fluorescence spectrometry. Anal. Chim. Acta 535,49-56 (2005)... 

The samples were analysed for total arsenic by hydride generation-atomic fluorescence spectrometry (HG-AFS) in the BGS laboratories in the UK. The detection limit was generally 0.25 or 0.5 pg L. Additional elements were measured by ICP-AES and in a few cases by ICP-MS. The samples were periodically interspersed with standard reference samples. The analytical data were combined with the site details and entered into a database. 

Dong, L.-M., and Yan, X.-P. (2005). On-line coupling of flow injection sequential extraction to hydride generation atomic fluorescence spectrometry for fractionation of arsenic in soils. Talanta 65, 627-631. 

Fuentes, E., Pinochet, H., De Gregori, L, and Potin-Gautier, M. (2003). Redox speciation analysis of antimony in soil extracts by hydride generation atomic fluorescence spectrometry. Spectrochim. Acta B 58, 1279-1289. 

N.V. Semenova, L.O. Leal, R. Forteza, V. Cerda, Multisyringe flow-injection system for total inorganic arsenic determination by hydride generation-atomic fluorescence spectrometry, Anal. Chim. Acta 455 (2002) 277. 

FI. Wu, Y. Jin, Y.-Q. Shi, S.-P. Bi, On-line organoselenium interference removal for inorganic selenium species by flow injection coprecipitation preconcentration coupled with hydride generation atomic fluorescence spectrometry, Talanta 71 (2007) 1762. 

Song, Q.G., Shi, J. and Zhao, K.L. (2005) Determination of trace bismuth in traditional Chinese medicines by hydride generation atomic fluorescence spectrometry. Fenxi Kexue Xuebao, 22 (1), 37-39. 

More recently for ultratrace determination and speciation of antimony compounds the so-called hyphenated instrumental techniques have been applied which combine adequate separation devices with suitable element-specific detectors. They include high-performance liquid chromatography (HPLC) connected on-line with heated graphite furnace (HGF) AAS (HPLC-HGF-AAS), hydride-generation atomic fluorescence spectrometry (HPLC-HG-AFS) or inductively coupled plasma (ICP) mass spectrometry (MS) (HPLC-ICP-MS) capillary electrophoresis (CE) connected to inductively coupled plasma mass spectrometry (CE-ICP-MS) and gas chromatography (GC) coupled with the same detectors as with HPLC. Reliable speciation of antimony compounds is still hampered by such problems as extractability of the element, preservation of its species information, and availability of Sb standard compounds (Nash et al. 2000, Krachler etal. 2001). Variants of anodic stripping voltammetry for speciation of antimony have also been applied (Quentel and Eilella 2002). 

Cava-Montesinos P, Ceeveea ML, Pastor A and De La Guaedia M (2003) Determination of arsenic and antimony in milk by hydride generation atomic fluorescence spectrometry. Talanta 60 787-799. 

HG-AFS hydride generation atomic fluorescence spectrometry HG-ICP-AES hydride generation inductively coupled plasma atomic emission spectrometry... 

Figure 3 Typical chromatograms showing speciation analyses of As(lll), As(V), MMA(V), DMA(V), MMA(lll), and DMA(lll) in deionized water (a), a urine sample (b), and the urine sample spiked with MMA(lll) (c), DMA(lll) (d), and As(V) (e). Separation was carried out on an ODS-3 column (15 cm X 4.6 mm, 3- rm particle size Phenomenex) with a mobile phase (pH 5.95) containing 5 mM tetrabutylammonium hydroxide, 3 mM malonic acid, and 5% methanol. The flow rate of the mobile phase was 1.2 ml/min. The column was maintained at 50°C. A hydride generation atomic fluorescence detector was used for detection of arsenic. Peaks labeled 1-6 correspond to As(lll), MMA(Itl), DMA(V), MMA(V), DMA(lll), and As(V) respectively. The urine sample was collected from a person 4 hr after the administration of 300 mg sodium 2,3-dimercapto-l-propane sulfonate (DMPS). For clarity, chromatograms were manually shifted on vertical axis. (Adapted from Ref. 96.)...

XC Le, M Ma. Short-column hquid chromatography with hydride generation atomic fluorescence detection for the speciation of arsenic. Anal Chem 70 1926-... 

Equation (1.26) shows how to calculate the LOD of a method at both the 95% and 99% confidence levels. You have measured the blank for a determination of arsenic in food samples by hydride-generation atomic fluorescence spectrometry. The blank values are ... 

Liquid chromatography/MS has also very recently been used for selenate and selenite determination and selenomethionine. And another recent liquid chromatographic hyphenated method, liquid chromatography-hydride generation atomic fluorescence spectrometry, has been used to determine selenite, selenate, selenocysteine, and selenomethionine in a single run. Reverse-phase and ion-exchange columns were used in series to accomplish the complex separation of all four of these analytes. And microchip capillary electrophoresis has most recently been used for determination of various Se-amino acids with negligible sample consumption . An excellent US Center for Disease Control summary of Se analytical methods is available. ... 

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