Liquid chromatography/ultraviolet/mass optimization

Cuyckens F and Claeys M. 2002. Optimization of a liquid chromatography method based on simultaneous electrospray ionization mass spectrometric and ultraviolet photodiode array detection for analysis of flavonoid glycosides. Rapid Commun Mass Spectrom 16(24) 2341—2348. 

Because purity determined by liquid chromatography (LC) or LC-mass spectrometry (LC-MS) with ultraviolet (UV) or evaporative light scattering detector (ELSD) detection represents only relative purity, the presence of impurities such as inorganics, materials with poor chromophores and/or good evaporative properties, and materials that tend to be retained by a guard column cannot be detected. Therefore, we measure the absolute purity of six representative compounds in each library at both optimization and production stages. 

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