Leukemia HL60 cells

We confirmed that this assay could be used to quantify active caspase-3 and active caspase-7 in other cell types. Active caspase-3 and active caspase-7 were quantified in the extracts from STS-treated human neuroblastoma (CHP-100 and SH-SY5Y) and promyelocytic leukemia (HL60) cells (Table 1). The amount of active caspase-3 was found to be much higher than the amount of active caspase-7 in these cell types. Very low or no amounts of active caspases were detected in control cells (cells not treated with STS). Active caspase-3 and active caspase-7 were also quantified in human breast adenocarcinoma (MCF-7) cells that do not express caspase-3 (28,38). No... 

Stroud, M. R., Handa, K., Salyan, M. E. K. et al. (1996b) Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 2. Characterization of E-selectin binding fractions, and structural requirements for physiological binding of E-selectin. Biochemistry, 35, 770-8. 

Haliclonacyclamine F (25), arenosclerin D (26), and arenosclerin E (27) have been recently isolated from the sponge Pachychalina alcaloidifera endemic in Brazil [26]. The alkaloids 25-27 were isolated from the cytotoxic, antibiotic, and antituberculosis MeOH crude extract of P. alcaloidifera by a series of separations on silica-gel and cyanopropyl-bonded silica-gel columns. The structures of compounds 25-27 were established by the same approach employed for the structural elucidation of haliclonacyclamine E (13) and arenosclerins A-C (14-16) [18], as well as by comparison with NMR data for this last series of alkaloids. The alkaloids 25-27 displayed moderate cytotoxic activity against SF295 (human CNS), MDA-MB435 (human breast), HCT8 (colon), and HL60 (leukemia) cancer cell lines. 

Sakurada K, Zheng B, Kuo JF (1992) Comparative effects of protein phosphatase inhibitors (okadaic acid and calyculin A) on human leukemia HL60, HL60/ADR and K562 cells. Biochem Biophys Res Commun 187 488-492... 

Shoji M, Raynor RL, Eerdel WE, Vogler WR, Kuo JF (1988) Effects of thioetherphos-pholipid EM41440 on PKC and phorbol ester-induced differentiation of human leukemia HL60 and KG-1 cells. Cancer Res 48 6669-6673... 

McCarthy, D.M. et al. (1983) 1,25-dihydroxyvitamin D3 inhibits proliferation of human promyelocytic leukaemia (HL60) cells and induces monocyte-macrophage differentiation in HL60 and normal human bone marrow cells. Leukemia Research, 7, 51-55. 

Inhibition of growth and induction of differentiation of promyelocytic Leukemia HL60 by the carotenoids of Crocus sativus has been studied by Tarantilis et al [19]. The IC50 values found, were 0,8 muM for dimethyl crocetin, 2 muM for crocetin and crocin respectively, and 5 muM induction of differentiation of HL60 cells. 

Concerning NO implication in hematopoietic cancer cells apoptosis, the situation is less clear. In U937 and HL60 cells, NO effects are very different although both cell lines have been established in culture from promyelocytic leukemia. NO-induced apoptosis in U937 cells (p53 negative cells) is a p53 and caspase independent pathway but is controlled by Bcl-2 [124]. In these cells NO inhibits caspase 3 by S-nitrosation... 

HL60 cells (a human promyelocytic leukemia cell line). 

Human myeloid leukemia cell line (HL60 cells, ATCC 240-CCL American Type Culture Collection, Rockville, MD). Upon receipt, HL60 cells should be stored in liquid nitrogen (see Note 1). 

Shi L et al (1996) Granzyme B induces apoptosis and cyclin A-associated cyclin-dependent kinase activity in all stages of the cell cycle. J Immunol 157 2381-2385 Shimizu T et al (1995) Unscheduled activation of cyclin Bl/ Cdc2 kinase in human promyelocytic leukemia cell line HL60 cells undergoing apoptosis induced by DNA damage. Cancer Res 55 228-231... 

As for medical applications two types of succinoyl trehalose lipids produced by R. erythropolis SD-74 were found to induce cell differentiation instead of cell proliferation in the human promyelocytic leukemia cell line HL60. Both compounds induced HL60 to differentiate into monocytes. Furthermore, they also inhibited protein kinase C (PKC) activities of HL60 cells indicating potential for antitumoral agents [33], Other investigations showed that... 

The antiproliferative properties of clavulone I (88) were studied in greater detail using the HL60 leukemia cell line [128]. Application of the natural product at 0.4 pM (0.2 pg ml 1) irreversibly decreased the growth rate of these cells to half that of controls. Complete growth inhibition was observed at about 1.0 pM and cytotoxicity was observed at higher concentrations. 

HETE, 12-hydroxyeicosatetraenoic acid HL60, human promyelocytic leukemia cells... 

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