Binding to selectin

McEver RP, Cummings RD. Role of PSGL-1 binding to selectins in leukocyte recruitment. J Clin Invest 1997 100 S97-S103. 

Glycosyltransferases assemble polysaccharides from their monomeric units by adding activated nucleotide sugars to the growing carbohydrate chain [19]. The chemistry of glycosyltransferases is well known [20-22] and is illustrated in the biosynthesis of sialyl Lewis [23], an essential contributor to the cell adhesion process. In fact, without both the siahc acid and fucose components, the sialyl Lewis remnant is incapable of binding to selectins and effecting cell adhesion necessary for leukocyte extravasation [24-29]. 

Investigations of the Natural Carbohydrate Determinants That Bind to Selectins. . 222... 

First, B16BL6 cells were co-injected into a tail vein of mice with either sLe -incorporated liposomes, which may bind to selectins, or with an Arg-Gly-Asp RGD-related peptide, DGRGDS conjugated with trimesic acid [Ar(GRGDS)3], which is known to suppress interaction with certain integrins. Both sLe -liposome and RGD-related peptide suppressed actual metastasis of B16BL6 cells to the lungs. 

We then examined the trafficking of B16BL6 cells in the presence of these inhibitors. As shown in Fig. 14.4(a), accumulation of [2- F]FDG-labeled B16BL6 cells in the lungs was suppressed in the presence of sLe -liposome, but was not affected by the liposome modified with methylated sLe (Me-sLe ), which lacked the ability to bind to selectins. Neither the Me-sLe -hposome nor the nonmodified liposome suppressed B16BL6 metastasis to the lungs. Interestingly, the RGD-related peptide did not reduce the accumulation... 

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