Klebsiella pneumoniae IFO

Dynamic kinetic resolution of a -alkyl-jS -keto esters was conducted successfully using biocatalysts (Fig. 10.42(b)).For the reduction of ethyl 2-methyl-3-oxobutanoate, a method of extensive screening was used to find the suitable microorganism. As a result, Klebsiella pneumoniae IFO 3319, out of 450 bacterial strains, was found to give the corresponding (2i ,3S)-hydroxy ester with 99% de and >99% ee in kilogram scale quantitatively. 

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