In vitro cell interactions

Dunn, S. E., Brindley, A., Davis, S. S., Davies, M. C., and Ilium, L. (1994), Polystyrene-poly (ethylene glycol) (PS-PEG2000) particles as model systems for site specific drug delivery. 2. The effect of PEG surface density on the in vitro cell interaction and in vivo biodistribution, Pharm. Res., 11,1016-1022. 

Zhou,J., Ertel, S.I., Buettner, H.M. and Kohn,J. (1994) Evaluation of tyrosine-derived pseudo-poly(amino acids) In vitro cell interactions. 20th Annual Meeting of the Society for Biomaterials. Boston MA, Society for... 

The rat glial cell is a crucial cell in the nervous system, which not only connects and supports nerve components but also provides growth factors and distributes nutrients throughout the nervous system [41], Thus these cells were selected to study in vitro cell interactions with nerve repair scaffolds. The cells were seeded onto... 

Methods for quantifying both the transcellular diffusion and concurrent metabolism of drugs and the unusual transcellular diffusion of membrane-interactive molecules coupled with the influence of protein binding are described in detail. To demonstrate the utility of cultured cell monolayers as a tool for basic science investigations, a subsection is devoted to the elucidation of rate-determining steps and factors in the passive diffusion of peptides across biological membranes. The chapter concludes with a discussion on the judicious use of in vitro cell monolayer results to predict in vivo results. 

It is difficult to simulate in vitro the interaction of all of the various cell types and modulators of immune function that make up the in vivo system. 

Nimmagadda A, Thurston K, Nollert MU, McFetridge PSF (2006) Chemical modification of SWNT alters in vitro cell-SWNT interactions. J. Biomed. Mater. Res. A 76A 614-625. 

The clinical relevance of data obtained from studies with single compounds is questionable, because most studies were performed in in vitro systems, limiting the predictability of the effects of the examined compounds in vivo. Moreover, some polyphenolics, such as quercetin, were shown to interact with the absorption or metabolism of drugs only at very high concentrations (50-100 pmol/L), which are likely to exceed the expected in vivo concentration after the consumption of a moderate amount of a grapefruit/ citrus product. Also, flavonoids have been demonstrated to potentially induce apoptosis in cell lines at concentrations comparable to those used for some in vitro drug interaction studies (64-66). This potentially could have impaired the investigation of enzyme and transporter activities. 

Classic in vitro cell culture models were used earlier to test the permeability of drugs themselves (9). Nowadays, these systems display not only an alternative to animal testing in respect to ethical concerns, but are also applied as advanced models to develop new delivery strategies and to study drug delivery properties of nanoparticulate carriers or chemical delivery enhancers. Thereby, interactions with the biological barrier, specifically binding, uptake, and transport can be evaluated in a well-defined and standardized system. While oral delivery of drugs is still the commonly used and most accepted method of choice due to the... 

Chishima, T., Miyagi, Y, Li, L., Tan, Y, Baranov, E., Yang, M., Shimada, H., Moossa, A. R. and Hoffman, R. M. (1997e) Use of histoculture and green fluorescent protein to visualize tumor cell host interaction. In Vitro Cell Dev. Biol. Anim. 33, 745-747. 

Whilst the use of standard tissue culture flasks in glass or plastic has provided much of the data on which our understanding of in vitro cell biology is based, a very wide range of novel culture formats are now available for the benefit of those in basic research and biotechnology. Some of these approaches, notably filterwell culture, are now fairly commonplace but others, such as microcontainers and the RWV system, remain to have their full potential identified. Recent developments in the area of 3D cell culture have been rapid. It is evident that there is still much to be learned about the way cell function is modulated in 3D cell-cell and cell-matrix interactions. 

The importance of substrate surface chemistry has been extensively studied and will not be described in detail here. The cellular environment emphasizes the importance of neighboring cells. Living cells constantly communicate with other cells in their surroundings and this cell-cell interaction partially explains the success of in vitro cell co-cultures in better maintaining the viability and normal functions of primary hepatocytes, which was discussed in Section 25.4. In this section we focus only on the microfluidic control of extracellular environment, and the influence of substrate topography on cell functions. 

George UM, Ashna U, Kumar SSP, Nandkumar AM (2013) Effect of tobacco extract on surfactant synthesis and its reversal by retinoic acid-role of cell-cell interactions in vitro. In Vitro Cell Dev Biol Anim 49(4) 260-269... 

Although both BBMECs and PBMECs are widely accepted as suitable in vitro models of the BBB, there are some limitations to the utility of these models. Over the years, the in vitro cell culture models have proven to be fairly reliable predictors of BBB permeability. However, as with any in vitro model, confirmation of results using an animal model is recommended. One should also be aware that substrate-transporter interactions have been shown to be altered in allelic variants... 

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