Immunochemical analysis

S.J. Gee, B.D. Hammock, and J.M. Van Emon, Environmental Immunochemical Analysis for Detection of Pesticides and other Chemicals a User s Guide, Nyes Publications, Westwood, NJ (1997). 

Yoshida T, Sano T, Kanuma T, et al. Immunochemical analysis of HPV LI capsid protein and pl6 protein in liquid-based cytology samples from uterine cervical lesions. Cancer (Cancer Cytopathol.) 2008 114 83-88. 

Stenoien, D. S. and Brady, S. T. Immunochemical analysis of kinesin light chain function. Molec. Biol. Cell 8 675-689, 1997. 

Wheeler CW, Park SS, Guenthner TM (1990) Immunochemical analysis of a cytochrome P-450IA1 homologue in human lung microsomes. Mol Pharmacol 38(5) 634—643. 

Oudin, J. (1980) Immunochemical analysis by antigen-antibody precipitation in gels. Methods Enzymol. 70,166-198. 

Hershko, A., Eytan, E., Ciechanover, A., and Haas, A.L. (1982). Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells Relationship to the breakdown of abnormal proteins. J. Biol. Chem. 257, 13964-13970. 

Protein quantification is an important step for handling protein samples for isolation and characterization, and is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. The methods included in this unit are colorimetric measurements, whose procedures are faster, simpler, and less laborious than those based on estimation of total nitrogen content (vnitbi.2). 

Oudin, J. (1960). Allotypy of rabbit serum proteins. I. Immunochemical analysis leading to the individualization of seven main allotypes. J. Exp. Med. 112,107-124. 

Gee, S.J., B.D. Hammock, and J. Van Emon (1994). A User s Guide to Environmental Immunochemical Analysis. NTIS PB95-138327, USEPA/540/R-94/509. Washington, DC US Government Printing Office. 

Nichkova, M., M. Germani, and M.P Marco. 2008. Immunochemical analysis of 2,4,6-Tribromophenol for assessment of wood contamination. J. Agric. Food Chem. 56 29-34. 

Nichkova, M. and M.R Marco. 2005. Development and evaluation of C18 and immunosorbent solid-phase extraction methods prior to immunochemical analysis of chlorophenols in human urine. Anal. Chim. Acta 533 67-82. 

Hock, B. 2002. Immunochemical analysis of water pollutants. Acta Hydmchim. Hydrobiol. 29 375-390. 

Cost Effectiveness. As with the other advantages of immunochemical analysis, cost may be quite variable. Reagent costs for several automated systems have been estimated at under 1.25 per sample. The cost is obviously much lower for less sophisticated assay systems, especially if some reagents are prepared in house. A major consideration is the expense of new instrumentation. For dedicated or automated instrumentation for either RIA or ELISA procedures, the cost may be 50-100,000. However, most analytical laboratories already have the basic instrumentation needed for immunoassays. Moderate sensitivity can be obtained through the use of numerous procedures such as radial immunodiffusion and hemagglutination. These procedures require no expensive equipment or reagents and they may be very useful in areas where equipment acquisition or maintenance is a problem. 

In many cases, those compounds which are most difficult to assay by classical procedures because of numerous polar functionalities and poor volatility are the very compounds which lend themselves most readily to immunochemical analysis (4). One can also predict that the number of pesticides marketed with a high... 

Hourihane, J.O B, Miemann, L.M., Hlywka, J.J., and Hefle, S.L. 2000. Immunochemical analysis of retail foods labeled as may contain Peanut or other similar declaration Implication for food allergic individuals. J Allergy Clin Immunol 105 188. 

Elsayed, S. and Apold, J. 1983. Immunochemical analysis of cod fish allergen M Location of the immunoglobulin binding sites as demonstrated by the native and synthetic peptides. Allergy 38 449 159. 

In 1970, Eckman et al. devised the automation of a quantitative immunochemical analysis of transferrin (El). In this automated flow system, diluted samples were allowed to react with antitransferrin antiserum serially and the degree of light scattering of the resulting turbidity was measured in the fluorometer, used as a nephelometer. The optimal conditions for nephelometry were extensively studied. Subsequently, Buffone reported... 

Immunochemical analysis of Taenia taeniaeformis antigens expressed in Escherichia coli. Parasitology, 93 599-610. 

Hanuszkiewicz, A., Hiibner, G., Vinogradov, E., Lindner, B., Brade, L., Brade, H., Debarry, J., Heine, H., Holst, O. Structural and immunochemical analysis of the lipopolysaccharide from Acinetobacter Iwoffii F78 located outside Chlamydiaceae with a Chlamydia-specific lipopolysaccharide epitope. Chem Eur J 14 (2008) 10251-10258. 

Sindhu, S.S., Brewin, N.J., Kannenberg, E.L. Immunochemical analysis of lipopolysaccharides from free-living and endosymbiotic forms of Rhizobium leguminosarum. J Bacteriol 172 (1990) 1804-1813. 

In the third part, methods for retrospective detection of exposure to toxic scheduled chemicals using mass spectrometric and immunochemical analysis methods are discussed. The described methods are applied to human origin samples. These methods are essential when in cases of use, or allegations of use, previous presence or absence of toxic chemicals need to be confirmed. Identification of CWC-related chemicals provides key supporting evidence of noncompliance with the CWC. 

Mass Spectrometric and Immunochemical Analysis of Covalent Adducts to Proteins and DNA. 433... 

Proteins such as cytochrome c are not good target antigens for the determination of species of origin because the structures of these proteins in evolutionarily distant species are very similar. Serum albumin, on the other hand, is a rapidly changing protein (Table I) that is amenable to complete immunochemical analysis with both polyclonal and monoclonal antibodies (14-16). Albumins from thousands of pairs of vertebrate species have been compared immunochemically, and the results have been used to measure evolutionary distances (22). 

Guengerich FP, Turvy CG (1991) Comparison of levels of several human microsomal cytochrome P-450 enzymes and epoxide hydrolase in normal and disease states using immunochemical analysis of surgical liver samples. /... 

Immunochemical Analysis by Antigen-Antibody Precipitation in Gels... 

The author s aims are a) to classify as rationally as possible the techniques of immunochemical analysis by antigen-antibody precipitation in gels (,b) to state the more or less general principles and laws which hold true for all or part of the techniques (c) to help the reader in understanding the bases of the different techniques in a way that does not require any special knowledge of physics and (d) to give a few examples to illustrate that this understanding of the principles and laws, plus an analysis of the reactions may raise, and eventually solve, problems of appreciable importance. 

---