Fibroblastic cell line

Rather scanty evidence exists for the participation of free radicals in Alzheimer s disease and Down s syndrome. However, more recendy, reports have appeared that suggest possible free-radical involvement in the pathogenesis of these two conditions. Zemlan et al. (1989) repotted that the activity of the free-radical scavenging enzyme, SOD, was significantly increased in fibroblast cell lines derived from familial Alzheimer s and Down s patients. They hypothesized that the elevation in SOD activity observed in the Alzheimer patients supports the theory that paired helical filaments are formed by free-radical hydroxylation of proline residues. They further su ested that SOD levels might also be increased in the brains of Alzheimer s and Down s patients, and that the increase in SOD may reflect an enhanced generation of free radicals. 

Ratnam, S. and Kent, C. (1995) Early increase in choline kinase-activity upon induction of the H-Ras oncogene in mouse fibroblast cell-lines. Archives of Biochemistry and Biophysics 323, 313—322. 

TGF-P was first described as a growth factor capable of inducing transformation of several fibroblast cell lines (hence the name TGFs). It is now recognized that TGF-P actually... 

Much less is known about the cytotoxic and antiproliferative effects of the 20(S)-PPT family of ginsenosides. Ginsenoside Rhi has been reported to inhibit proliferation of the NIH 3T3 mouse fibroblast cell line but did... 

Fig. 1. (opposite page) Distribution of FITC-conjugated BSA in various fibroblast cell lines under different fixation/permeabilization regimes. (A-D) Protein distribution in living cells (A) PtKj, (B) CHO, (C) 3T3, and (D) HeLa cells. The protein is excluded from the nuclei of all cells. (E-H) Protein distribution in cells extracted for 10 min with 0.1% Triton X-100 before fixation for 30 min with 3.7% formaldehyde (E) PtKi, (F) CHO, (G) 3T3, and (H) HeLa cells. Nuclear fluorescence is seen in (E) PtKj and (G) 3T3 cells. (I-L) Protein distribution in cells extracted for 10 min with 1% Triton X-100 before fixation for 30 min with 3.7% formaldehyde (I) PtKj, (J) CHO, (K) 3T3, and (L) HeLa cells. No fluorescence is detected in the cells with the exception of some nuclear fluorescence seen in (L) HeLa cells. (M-P) Protein distribution in cells fixed for 30 min with 3.7% paraformaldehyde before permeabilization for 10 min with 0.1% Triton X-100. Fluorescence is seen primarily in the cytoplasm with the exception that nuclear fluorescence is seen in (M) PtKi and (N) CHO cells. (Q-T) Protein distributions in cells fixed for 5 min with 90% methanol, 50 vaM EGTA at -20°C (Q) PtKj, (R) CHO, (S) 3T3, and (T) HeLa cells. All cells show an overall low fluorescence, fibrous-textured cytoplasmic fluorescence, and bright staining at the periphery of the nucleus. 10 mm per scale division (black bar). (Reproduced with permission from ref. 6.)... 

SU-6656 (12) [113-115] is a potent inhibitor of Src kinase (as well as Lck, Fyn, and Yes kinases) and is an effective inhibitor of PDGF-stimulated DNA synthesis and Myc induction in a fibroblast cell line. SU-6656 has also been a useful tool for investigating the role of Src and Ras-ERK signal transduction in Src-transformed cells with respect to Racl, as well as implicating Vav2 and Tiaml as downstream effectors of Src to modulate Racl-dependent pathways. In endothelial cells, SU-6656 is effective in increasing radiation-induced apoptosis and vascular endothelium destruction, and in vivo studies have... 

The phototoxicity test 3T3 NRU was proposed in 1994 and is so far the only in vitro method that has been validated by European regulatory authorities for predicting the photoirritant potential of substances [5,40,41]. In this test, the mouse fibroblasts cell line Balb/c 3T3 is exposed to simulated solar UV (or, more frequently, solar UVA) in the presence of the test compound after an incubation of 1 h in the dark. Evaluation of cytotoxicity is performed 24h post-exposure using the neutral red uptake (NRU) method. N RU permits to distinguish live and dead cells, since intact cells retain this dye (detailed method in INVITOX protocol 78). The validation was performed with substances selected on the basis of their in vivo photoirritant or phototoxic properties. Some of these structures are shown in Table 19.1. 

Use, in standard culture conditions, an established cell line sensitive to the cytopathic elfect of a suitable virus (a human diploid fibroblast cell line, free of microbial contamination, responsive to interferon and sensitive to encephalomyocarditis virus, is suitable). 

Danford, N. (1985) Tests for chromosome aberrations and aneuploidy in the Chinese hamster fibroblast cell line CHl-L. In Ashby, J., de Senes, F.J., Draper, M., Ishidate, M., Jr, Margolin, B.H., Matter, B.E. Shelby, M.D., eds. Progress in Mutation Research, Volume 5, Evaluation of Short-Term Tests for Carcinogens. Report of the International Programme on Chemical Safety s Collaborative Study on in vitro assays, Amsterdam, Elsevier Seienee, pp. 397 11... 

Fig. 2. Effect of serum concentration on the attachment and spreading of BHK-21 cells onto TCP2 surface. BHK-21 cells were seeded in media containing the indicated concentrations of intact serum (open squares), Fn-depleted serum (triangles). Vn-depleted serum (circles), or serum-free medium alone (the single closed square) and the attachment panel (A) and spreading panel (B) of the cells were determined after 90 min culture on TCP (panel (A, B)) Mean SEM. (Reproduced from J. Biomed. Mater. Res. [Ref. 11 Role of serum vitronection and fibronectin in adhesion of fibroblasts following seeding onto tissue culture polystyrene] through the courtesy of John Wiley Sons, Inc.) BHK-21 Fibroblast cell lines from Baby Hamster Kidney 2 Similar results on Primaria are also presented in [Ref 11]...

Rassoulzadegan, M., Naghashfar, Z., Cowie, a., Carr, A., Grisoni, M., Kamen, R., and CUZIN, F. (1983). Expression of the large T protein of polyoma virus promotes establishment in culture of normal rodent fibroblast cell lines, Proc. NatL Acad. Sci. 80, 4354. 

Danford, N. (1985) Tests for chromosome aberrations and aneuploidy in the Chinese hamster fibroblast cell line CHl-L. Prog. Mutat. Res., 5, 397 411... 

Bladier, C., Wolvetang, E.J., Hutchinson, P, de-Haan, J.B. Kola, I. (1997) Response of a primary human fibroblast cell line to H2O2 senescence-like growth arrest or apoptosis Cell Growth Differ, 8, 589-598... 

Ishidate, M., ed. (1983) The Data Book of Chromosomal Aberration Tests In Vitro on 587 Chemical Substances Using a Chinese Hamster Fibroblast Cell Line (CHL Cell), Tokyo, Realize Inc. 

Figure 1. Pedigree pattern of the B family. Relationship of the proband (T.B.) with the clinical phenotype of homozygous familial hypercholesterolemia to her other relatives whom we studied is shown. Lipoprotein patterns were determined after ultracentrifugation using NIH outpoints (51). F indicates that fibroblast cell lines were established from skin biopsies. Males, H Females, O.

The tetraazatriphenylene chromophore attached to the cyclene ring acted as an efficient sensitiser for Eu3+ and Tb2+ emission but also intercalated between the base pairs of DNA. The complexes were tested as cellular imaging and reactive probes using the mouse fibroblast cell line. The complexes were quickly taken up by the fibroblast cells and localised in nucleus and around the cell membrane. The process was visualised by fluorescence microscopy. Photolysis at 340 nm and 350 nm damaged plasmid supercoiled DNA producing nicked (form II) and linear (form III) DNA. DNA damage is known to induce apoptotic cell death and these complexes may be therefore considered for the development as therapeutic probes, for example in the treatment of accessible tumours, such as skin melanoma. 

Before implantation several in vitro tests were performed. For evaluation of a possible toxic reaction, we investigated the material and the whole devices in vitro with cell culture methods. Direct contact and extraction tests with a mouse fibroblasts cell line (L 929) and a neuroblastoma cell line (neuro-2-a) were performed according to the international standard ISO 10993 ( Biological Evaluation of Medical Devices ). The materials and devices showed no toxicity, i.e. no significant differences in membrane integrity of the cell membranes, mitochondrial activity and DNA synthesis rate. The neuro-2-a cell line is so sensitive that even small changes in process technology are detectable. The flexible polyimide structures proved to be non toxic. 

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