Mouse colon

Younes, M., Trepkau, H.D. and Siegers, C.P. (1990). Enhancement by dietary iron of lipid peroxidation in mouse colon. Res. Commun. Chem. Pathol. Pharmacol. 70, 349-354. 

Motomura Y, Khan WI, El-Sharkawy RT, et al. Induction of a fibrogenic response in mouse colon by overexpression of monocyte chemoattractant protein 1. Gut 2006 55(5) 662-670. 

The application of flavonoids for the treatment of various diseases associated with free radical overproduction is considered in Chapter 29. However, it seems useful to discuss here some studies describing the activity of flavonoids under certain pathophysiological conditions. Oral pretreatment with rutin of rats, in which gastric lesions were induced by the administration of 100% ethanol, resulted in the reduction of the area of gastric lesions [157]. Rutin was found to be an effective inhibitor of TBAR products in the gastric mucosa induced by 50%i ethanol [158]. Rutin and quercetin were active in the reduction of azoxymethanol-induced colonic neoplasma and focal area of dysplasia in the mice [159], Chemopreventive effects of quercetin and rutin were also shown in normal and azoxymethane-treated mouse colon [160]. Flavonoids exhibited radioprotective effect on 7-ray irradiated mice [161], which was correlated with their antioxidative activity. Dietary flavones and flavonols protected against the toxicity of the environmental contaminant dioxin [162], Rutin inhibited ovariectomy-induced osteopenia in rats [163],... 

T. Nakanishi, T. Hatanaka, W. Huang, P. D. Prasad, F. H. Leibach, M. E. Ganapathy, and V. Ganapathy. Na+- and Cl--coupled active transport of carnitine by the amino acid transporter ATB(0,+) from mouse colon expressed in HRPE cells and Xenopus oocytes. J. Physiol. 532 297-304 (2001). 

Cohen, P. S., Rossoll, R., Cabelli, V. J., Yang, S. L., and Laux, D. C. (1983). Relationship between the mouse-colonizing ability of a human fecal Escherichia coli strain and its ability to bind a specific mouse colonic mucous gel protein. Infect. Immun. 40,62-69. 

Richter, F., Newmark, H. L., Richter, A., Leung, D., and Lipkrn, M. (1995). Inhibition of Western-diet induced hyperproliferation and hyperplasia in mouse colon by two sources of calcium. Carcinogenesis 16, 2685-2689. 

Koval, T.M. (1984) Selective inhibition of replicative and repair DNA synthesis in mouse colon... 

Oprins, J.C., Van der Burg, C., Meijer, H.P., Munnik, T. and Groot, J.A., 2002, Tumor necrosis factor alpha potentiates ion secretion induced by histamine in a human intestinal epithelial cell line and in mouse colon involvement of the phospholipase D pathway. Gut 50 314—321. 

Kim, D.J. et al., Chemoprevention by lycopene of mouse lung neoplasia after combined initiation treatment with DEN, MNU, and DMH, Cancer Lett, 120, 15, 1997. Risio, M. et al.. Apoptosis, cell replication, and Western-style diet-induced tumori-genesis in mouse colon. Cancer Res., 56, 4910, 1996. 

Melanin Pigment Producing Mouse Colon Cancer... 

Cells COS-7 cells (the African green monkey kidney fibroblast cell line), S-180 cells (mouse sarcoma), Meth-A fibrosarcoma cells (mouse fibrosarcoma), Jurkat cells (human T cell line). Colon 26 cells (mouse colon adenocarcinoma), B16BL6 cells (mouse meranoma). Human umbilical vein endothelial cells (HUVEC) (Kurabo Industries, Osaka, Japan). 

Fig. 3. Property of gene delivery with BLs and US exposure (a) Schema of transfection mechanism by BLs and US. The mechanical effect based on the disruption of BLs by US exposure, which results in generation of some pores on plasma membrane, is associated with direct delivery of extracellular plasmid DNA into cytosol, (b) Luciferase expression in COS-7 cells transfected by BLs and US. COS-7 cells (1x10 cells/500 pLAube) were mixed wifh pCMV-Luc (5 pg) and BLs (60 pg). The cell mixture was exposed with US (Frequency 2 MHz, Duty 50%, Burst rate 2 Hz, Intensity 2.5 W/ cm. Time 10 s). The cells were washed and cultured for 2 days. Affer fhaf, luciferase acfivify was measured, (c) Effecf of US condition on transfection efficiency with BLs. COS-7 cells were exposed with US (Frequency 2 MHz, Duty 50%, Burst rate 2 Hz, Intensity 2.5 W/cm Time 0,1, 5,10 s) in the presence of pCMV-Luc (0.25 pg) and BLs (60 pg). Luciferase activity was measured as above, (d) Effect of serum on transfection efficiency of BLs. COS-7 cells in the medium containing EBS (0,10, 30, 50% (v/v)) were treated with US (Erequency 2 MHz, Duty 50%, Burst rate 2 Hz, Intensity 2.5 W/cm, Time 10 s), pCMV-Luc (0.25 pg) and BLs (60 pg) or transfected with lipoplex of pCMV-Luc (0.25 pg) and lipofectin (1.25 pg). (e) In vitro gene delivery to various types of cell using BLs and US. The method of gene delivery was same as above. S-180 mouse sarcoma cells, Colon26 mouse colon adenocarcinoma cells, B16BL6 mouse melanoma cells, Jurkat human T cell line, HUVEC human umbilical endothelial cells. Luciferase activity was measured as above. <10 RLU/mg protein, <10 RLU/mg protein Each data represents the mean S.D. n=3). L PEG-liposomes, LF Lipotectin...

Salim, E.I. et al., Low-dose-dependent carcinogenic potential of 2-amino-3-methylimid-azo[4,5-f quinoline in the immunodeficient (SCID) mouse colon, Nutr. Cancer, 34, 220, 1999. 

Augenlicht LH, Kobrin D (1982) Cloning and screening of sequences expressed in a mouse colon tumor. Cancer Res 42(3) 1088-1093... 

Acetylcholine, Mouse Colon Electrically evoked cholinergic and NANC Storr et al. 2004... 

Acetylcholine Mouse Colon Electrophysiology (intracellular Inverse agonist (SR 141716), knockout Storr etal. 2004... 

Storr M, Sibaev A, Marsicano G, Lutz B, Schusdziarra V, Timmermans JP, Allescher HD (2004) Cannabinoid receptor type 1 modulates excitatory and inhibitory neurotransmission in mouse colon. Am J Physiol Gastrointest Liver Physiol 286 G110-Gl 17... 

Mouse colon Inhibition of DNA repair + Koval 1984 12DMH... 

Mouse colon Sister chromatid exchange + Couch et al. 1986 12DMH... 

Guda K., Cui H., Garg S., Dong M., Nambiar P. R., Achenie L. E. and Rosenberg D. (2003). Multistage gene expression profiling in a differentially susceptible mouse colon cancer model. Cancer Letters 191, pp 17-25. 

Ohyama, W., Gonda, M., Miyajima, H., Kondo, K., Noguchi, T., Yoshida, J., Hatakeyama, S., Watabe, E., Ueno, Y., Hayashi, M., and Tokumitsu, T. (2002). Collaborative validation study of the in vivo micronucleus test using mouse colonic epithelial cells. Mutat Res 518, 39-45. 

Ogata N, Ataka K, Morino H, et al. 1999. Effect of wood creosote and loperamide on propulsive motility of mouse colon and small intestine. Pharmacology 59 212-220. 

In the chick/quail model system as well as in the mouse, colonization seems to occur in several waves, with cycles of 5 to 6 days. There is an initial period of stem cell influx, which may depend on the epithelial thymus elaborating an attractant factor, followed by a refractory phase, during which there is little stem cell influx, followed by another wave of colonization (Le... 

Aphidicolin (89) is a diterpenoid tetraol produced by the mold Cephalosporium aphidicola. In 1972, Hesp and co-workers reported its isolation and structure [50]. Aphidicolin (89) displays marked activity against Herpes simplex type I virus in cultures of human embryonic cells, as well as antifeedant properties. Furthermore, aphidicolin (89) exhibits antitumor activity in the C6 mouse colon and B16 mouse melanosarcoma screens and has been shown to inhibit the growth of leukemic T- and B-lymphocytes [51]. Such results have stimulated interest in clinical investigations of the effectiveness of 89 against human tumors. 

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