Human alveolar epithelial cells

Elbert KJ, Schafer UF, Schafers HJ, Kim KJ, Lee VHL, Lehr C-M (1999) Mono-layers of human alveolar epithelial cells in primary cultures for pulmonary drug delivery and transport studies. Pharm Res 16 601-608. 

Figure 11.2 Morphological differences between human alveolar epithelial cells in primary culture (A and C) and the A549 cell line (B and D). Cells are visualised by light microscopy (A and B) and immunofluorescence microscopy (C and D) using an antibody against a tight junctional protein, occludin.

Table 11.3 Summary of the Expression Pattern and Activities of Phase I and Phase II Metabolic Enzymes and Various Peptidases/Proteases in Human Peripheral Lung Tissues, Primary Rat AEC Culture and Human Alveolar Epithelial Cell Line, A549.

Fuchs S, Hollins AJ, Laue M, Schaefer UF, Roemer K, Gumbleton M, Lehr CM (2003) Differentiation of human alveolar epithelial cells in primary culture— Morphological characterisation and expression of caveolin-1 and surfactant protein-C. Cell Tissue Res 311 31-45... 

Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ (2007) Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro. Am J Respir Cell Mol Biol [Epub ahead of print] doi 10.1165/rcmb.2006-04100C... 

Ehrhardt C, Kim KJ, Lehr CM (2005) Isolation and culture of human alveolar epithelial cells. Methods Mol Med 107 207-216... 

Bur M, Huwer H, Lehr CM, Hagen N, Guldbrandt M, Kim KJ, Ehrhardt C (2006) Assessment of transport rates of proteins and peptides across primary human alveolar epithelial cell monolayers. Eur J Pharm Sci 28(3) 196-203... 

Endter S, Becker U, Damn N, Huwer H, Lehr CM, Gumbleton M, Ehrhardt C (2007) P-glycoprotein (MDR1) functional activity in human alveolar epithelial cell monolayers. Cell Tissue Res [Epub ahead of print] doi 10.1007/s00441-006-... 

Ehrhardt C, Kneuer C, Baldes C, Kim KJ, Lehr CM (2006) Albuterol is net absorbed across human alveolar epithelial cell monolayers. Proc Am Thor Soc 3A761... 

Recently, a monolayer of human alveolar epithelial cells was used to study the bioadhesive properties of lectins [37]. Lectins are sugar-recognizing adhesive molecules (they bind to epithelial cells) and are thought to increase the bioavailability of larger molecules by triggering vesicular transport processes. 

Elbert, K.J., U.F. Schafer, H.J. Schafers, K.J. Kim,V.H. Lee, and C.M. Lehr. 1999. Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies. Pharm. Res. 16 601-608. 

Deposition from serum-free medium culturing in serum-containing medium Fluorinated polymer Spatially separated coculture of human alveolar epithelial cells, human hepatoma cells, and mouse 3T3 fibroblast Photocatalytic removal of fluorinated polymer (mask lithography) 2009 [164]... 

Although interspecies differences can be circumvented through the use of primary cultures of human alveolar epithelial cells, routine use of these cells is limited by availability.58,60 Primary culture of rat type II alveolar epithelial cells is economical and demonstrates high reproducibility, and its phospholipid secretion is similar to that of human type II cells.43 P-gp is expressed in both human and rat type I cells but not in freshly isolated rat type II cells.61 It is assumed that alveolar type II cells are progenitors for regenerating type I cells in vivo, and type II cells in culture lose their cuboidal appearance, lamellar bodies, and microvilli, and the number of surfactant proteins decrease. Monolayers are formed in 5 to 8 days, and the transdifferentiation to type I-like cells is complete within 7-8 days, characterized by the development of attenuations, tighter junctions, and increasing expression of... 

Brack A, Abu-Dahab R, Borchard G, et al. Lectin-functionalized liposomes for pulmonary drug delivery interaction with human alveolar epithelial cells. J Drug Target 2001 9 241. 

Benfield TL, Lundgren B, Shelhamer JH, Lundgren JD. Pneumocystis carinii major surface glycoprotein induces interleukin-8 and monocyte chemoattractant protein-1 release from a human alveolar epithelial cell line. Eur J Chn Invest 1999 29 717-722. 

Lin Y, Zhang M, Bames PF. Chemokine production by a human alveolar epithelial cell line in response to Mycobacterium tuberculosis. Infect Immun 1998 66 1121-1126. 

Takeuchi R, Tsutsumi H, Osaki M et al. Respiratory syncytial virus infection of human alveolar epithelial cells enhances interferon regulatory factor 1 and interleukin-lbeta-converting enzyme gene expression but does not cause apoptosis. J Virol 1998 72(5) 4498-502. 

Interleukin-8 secretion from the human alveolar epithelial cell line A549 showed that dust samples from paper- and mail-sorting plants had a significant lower inflammatory potential as compared with dust from plants handling mixed household waste (Allermann and Poulsen 2000). No correlation was observed between the potency factor (IL-8 released per mg dust) and the concentration of endotoxin in the samples. 

Human alveolar epithelial cells (A549) and human lung microvascular endothelial cells cultured under hyperoxia (95% O2) + NO (derived from chemical donors) died after 4 to 5 days, whereas cells neither died nor divides in NO alone (Na-RULA et al. 1998). The toxic effect was clearly synergistic, and cell death did not occur via apoptosis. As an indicator of peroxynitrite formation, nitro-tyrosine-containing proteins at molecular masses of 25 and 35 kDa were found to be increased in A549 cells exposed to NO or NO + hyperoxia. 

Park, S., Lee, Y.K., Jung, M., et al., 2007. Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells. Inhalation Toxicology 19 (I), 59-65. 

Kemp, S. J. Thorley, A. J. Gorelik, J. Seckl, M. J. O Hare, M. J. Arcaro, A. Korchev, Y. Goldstraw, R Tetley, T. D., Immortalization of human alveolar epithelial cells to investigate nanoparticle uptake. American Journal of Respiratory Cell and Molecular Biology 2008, 39, 591-597. 

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