Surfactant protein B

Bruni, R., Taeusch, H.W., and Waring, A.J. Surfactant protein B Lipid interactions of synthetic peptides representing the amino-terminal amphipathic domain. Proc. Natl. Acad. Sci. USA 1991, 88, 7451-7455. 

Lipp, M.M., Lee, K.Y.C., Waring, A., and Zasadzinski, J.A. Fluorescence, polarized fluorescence, and Brewster angle microscopy of palmitic acid and lung surfactant protein B monolayers. Biophys. J. 1997, 72, 2783-2804. 

Seurynck, S.L., Johnson, M., and Barron, A. E. Simple helical peptoid analogues of lung surfactant protein B. 2003. (Manuscript in preparation). 

SAPA Saposin/surfactant protein-B A-type DOMAIN E(M) 0(0) 0(0) ... 

Clark JC, Wert SE, Bachurski CJ, et al. Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice. Proc Natl Acad Sci USA 1995 92(17) 7794 7798. 

Hawgood S. Surfactant protein B structure and function. Biol Neonate 2004 85(4) 285-289. 

Alveoli represent the primary site for gas exchange within the lung, and thus their health is vital for survival. Alveolar conditions with a primary genetic cause, such as surfactant protein-B (SP-B) deficiency and SP-C deficiency, are prime candidates for a rAAV-gene therapy approach. Diseases in which alveoli are damaged secondary to other defects might also be treated with gene transfer. Such conditions include environmental toxin exposure, infectious diseases, and adult respiratory distress syndrome (ARDS) (Table 4.1) (Rolls et al., 1997, 1998, 2001 Cheers et al 1999 Ruan et al., 2002). 

The surfactant protein B was unspecifically labelled with/ac-[99mTc(CO)3]+. The highly lipophilic protein spread over a hydrophilic surface and could have potential in the diagnosis of acute respiratory disease syndrome. The spreading properties of the labelled and native surfactant protein B were in coincidence, making labelled surfactant protein B a potential diagnostic radiopharmaceutical [122]. 

In some cases, it is desirable to have a pharmaceutical protein in an aggregated state because it is the bioactive form of the protein. An example of this is surfactant protein B (SP-B), a pulmonary surfactant protein necessary for normal lung function in neonatal infants. " The protein exists exclusively as a homodimer in which the monomers are linked by a disulfide bond. In studies investigating efficacy of the SP-B monomer compared with the dimer in transgenic mice, it was found that although the surfactant action was preserved in the monomeric form of the protein, altered lung hysteresis was noted. The authors concluded that SP-B dimerization is required for optimal lung function. 

Thompson, M.W. Surfactant protein B deficiency insights into surfactant function through clinical surfactant protein deficiency. Am. J. Med. Sci. 2001, 321 (1), 26-32. 

Nogee LM, Gamier G, Dietz HC, Singer L, Murphy AM, deMeUo DE, et al. A mutation in the surfactant protein B gene responsible for fatal neonatal respira-... 

Pryhuber GS. Regulation and function of pulmonary surfactant protein B. Mol Genet Metab 1998, 64, 217-228. 

O Reilly MA, Weaver TE, Pilot-Matias TJ et al (1989) In vitro translation, post-translational processing and secretion of pulmonary surfactant protein B precursors. Biochim Biophys Acta 1011 (2-3) 140-148... 

J. M. Klein, M. W. Thompson, J. M. Snyder, et al. Transient surfactant protein B deficiency in a term infant with severe respiratory failure. Journal of Pediatrics 132, 244 (1998). 

Khoor A, Whitsett JA, Stahlman MT, et al. Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma. Hum Pathol. 1999 30 695-700. 

Surfactant protein B (SP-B), an 8 kDa hydrophobic protein essential for surfactant function, is produced from the intracellular processing of the 381 amino acid residues, 40 kDa preprotein SP-B within the type II pneumocytes. 

Surfactant protein B deficiency worsened hyperoxic injury (95 % O2 at 1 atmosphere for 72 h) to the alveolar epithelium of SP-B" " mice (Tokieda et al. 1999). 

Effects of curcumin, an antioxidant lipophilic drug on membrane structure by Solid-State NMR Spectroscopy. xviii. The elfect of the cationic, helical peptide based on the essential lung surfactant protein B on oriented lipid bilayers characterized by H... 

Bohinski RJ, Huffman JA, Whitsett JA, Lattier DL. cis-Active elements controlling lung cell-specific expression of human pulmonary surfactant protein B gene. J. Biol... 

Manzanares, D., Rodriguez-Capote, K., Liu, S., Haines, T., Ramos, Y., Zhao, L., Doherty-Kirby, A., Lajoie, G., and Possmayer, F. 2007. Modification of tryptophan and methionine residues is implicated in the oxidative inactivation of surfactant protein B. Biochemistry 46 5604-5615. 

Williams GD, Christodoulou J, Stack J, et al. Surfactant protein B deficiency clinical, histological and molecular evaluation. J Paediatr Child Health 1999 35(2) 214-220. 

Nogee LM, de Mello DE, Dehner LP, et al. Brief report deficiency of pulmonary surfactant protein B in congenital alveolar proteinosis. N Engl J Med 1993 328(6) 406 10. 

Hamvas A, Nogee LM, Mallory GB Jr., et al. Lung transplantation for treatment of infants with surfactant protein B deficiency. J Pediatr 1997 130(2) 231-239. 

The synthetic 21-amino acid polypeptide KL4 [(KL4)4K] was developed as a pulmonary surfactant to be used in treatment of respiratory distress syndrome (RDS) in infants (IRDS) and in adults (ARDS). KL4 is designed to contain intermittent hydrophobic (Leucine) and hydrophilic (Lysine) regions to mimic the structure of the natural surfactant protein B (SP-B). The solution phase synthesis of KL4 is a challenging task. The protection and deprotection patterns, the possibility of diastereomer formation in every coupling step, the solubility (or lack of it) of the different fragments and the isolation of intermediates are among the potential problems that face such a synthesis. The process research work resulted in a successful and efficient large scale synthesis of KL4 which will be discussed. 

Pulmonary surfactant protein B BiceUar Upid mixtures 104... 

Breathing is enabled by lung surfactant, a mixture of proteins and lipids that forms a surface-active layer and reduces surface tension at the air-water interface in lungs. Surfactant protein B (SP-B) is an essential component of lung surfactant. Researchers probed the mechanism underlying the important functional contributions made by the N-terminal 7-residues of SP-B, a region sometimes called the insertion sequence . These studies employed a construct of SP-B, SP-B (1-25,63-78), also called Super Mini-B, which is a 41-residue peptide with internal disulfide bonds comprising the N-terminal 7-residue insertion sequence and the N- and C-terminal helixes of SP-B. CD, solution NMR, and SS NMR were used to study the structure of SP-B (1-25,63-78) and its interactions with phospholipid bilayers. Comparison of results for SP-B (8-25,63-78) and SP-B (1-25,63-78) demonstrates that the presence of the 7-residue insertion sequence induces substantial disorder near the center of the lipid bilayer. ... 

Yei S, Bachurski CJ, Weaver TE, Wert SE, Trapnell BC, Whitsett JA. Adenoviral-mediated gene transfer of human surfactant protein B to respiratory epithelial cells. Am J Respir Cell Mol Biol 1994 11 329-336. 

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