HT-29 human colon carcinoma

Initial experiments have shown that gemcitabine has a potent enhancing effect on the cellular effects of ionizing radiation reports with rodent EMT6 (26) cells and with HT-29 human colon carcinoma cell lines (27) showed that noncytotoxic concentrations of the drug lead to significant enrichment of radiation-induced cell lethality (Fig. 3). In the latter study it was found that the noncytotoxic concentrations of the drug (10 nM... 

The polyhalogenated monoterpenes 57-59 from the Spanish sea hare Aplysia punctata show identical cytotoxic properties against P-388 mice lymphoma and HT-29 human colon carcinoma (ED50 2.5 pg/ml), A-549 human lung carcinoma and MEL-28 human melanoma cell lines (ED50 1.5 pg/ml) [57]. 

Malevamide D 137, a highly cytotoxic peptide ester from a Hawaiian marine cyanobaeterium of Symploca hydnoides, showed IC50 values of 0.3-0.7 nM (0.2-0.5 ng/mL) against P-388 (mouse lymphoma), A-549 (human lung carcinoma), and HT-29 (human colon carcinoma) cell lines and 0.7 nM against the MEL-28(human melanoma) eell line. ... 

The cytotoxicities of the 3 -difluorovinyl-taxoids 29 were evaluated in vitro against MCF7-S, MCF7-R, HT-29 (human colon carcinoma), and PANC-1 (human pancreatic carcinoma) cell lines [37], The results are summarized in Table 5.5. 

Zhang, M., Liu, H., Guo, R., Ling, Y., Wu, X., Li, B., Roller, P. P., Wang, S., and Yang, D. (2003). Molecular mechanism of gossypol-induced cell growth inhibition and cell death of HT-29 human colon carcinoma cells. Biochem. Pharmacol. 66, 93-103. 

Next to rotenone, the Streptomyces mobaraensis metabolite piericidin (also known as shaoguanmycin B), specifically piericidin Ai (44), is perhaps the most studied NADH—U(i oxidoreductase inhibitor and is commonly used as a pharmacological probe of the role of complex I in the mitochondrial ETC. For example, glucose deprivation causes upregulation of ER chaperone protein GRP78 and induces etoposide resistance in human cancer cells. GRP78 is also known to protect tumor cells from apoptosis induced by topoisomerase inhibitors. " Studies have shown that glucose-deprived etoposide-resistant HT-29 human colon carcinoma cells are uniquely sensitive to piericidin A. ... 

Although no bioactivity data was originally reported for sodwanones A-F, sodwanones G-I were found to be cytotoxic to four cancer cell lines, P-388 (murine leukaemia), A-549 (human lung carcinoma), HT-29 (human colon carcinoma) and MEL-28 (human melanoma) (Table 3) [64], The apparent selective toxicity of 57 and 58 in the A-549 cancer assay is of interest. The anti-tumor activity of sodwanones A, G, and H has been patented [67]. 

While compounds 106-108 exhibited cytotoxicity (IC50 = 1.7-2.1 pM) to P-388 mouse leukaemia, A-549 human lung carcinoma, MEL-28 human melanoma and HT-29 human colon carcinoma cell lines, only 106 showed enhanced cytotoxicity against the latter cell line (IC50 = 0.2 pM)... 

Methyl-1,3-dihydropyrrolo[ 1,2-c]thiazole-6,7-biscarbamates have been described as active compounds against murine P388 lymphocytic leukemia <87JMC2109>. Several 5-aryl-2,3-dihydro-pyrrolo[2,l-fe]thiazole-6,7-dimethanol-6,7-bis(isopropylcarbamate)s were tested for growth inhibitory activity with the HL-60 human promyelocytic leukemia cell line. The 5-phenyl, 5-(4-fluorophenyl), and 5-(3,4-dichlorophenyl) have antileukemic activity. These compounds were also cytotoxic to HT-29 human colon carcinoma cells <88JMC1427>. 

M. Walsh, S. Hanna, J. Sen, S. Rawal, C. Cabral, A. Yurkovetskiy, R. Fram, T. Lowinger, W. Zamboni, Pharmacokinetics and antitumor efficacy of XMT-1001, a novel, polymeric topoisomerase I inhibitor, in mice bearing HT-29 human colon carcinoma xenografts, CUn. Cancer Res. 18 (2012) 2591-2602. 

Figure 8.13 Inhibition ofiNOS protein by pterostilbene in HT-29 colon carcinoma cells. HT-29 human colon carcinoma cells were grown in complete medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/ streptomycin) at 37 °C, 5% CO2. At day 0, HT-29 cells were plated in 100 mm dish (2 x 10 cells...

Synthesis of (-)-Haouamine A Haouamine A (193) was isolated in 2003 from a marine ascidian Aplidium haouarianum) and displayed potent and selective cytotoxic activity against the HT-29 human colon carcinoma cell Une. Aube et al. [29] have culminated a formal... 

K.H. Kang, C.S. Kong, Y. Seo, M.M. Kim, S.K. Kim, Anti-inflammatory effect of coumarins isolated from Cotydalis heterocarpa in HT-29 human colon carcinoma cells. Food Chem. Toxicol. 47 (2009) 2129-2134. 

Conicol was a meroterpenoid isolated from the ascidian Aplidium conicum collected from Tarifa Island. In Salva et al. s study, cytotoxicity assays performed on P-388 mouse lymphoma, A-549 human lung carcinoma, and HT-29 human colon carcinoma cell lines of the organic extract of A. conicum showed a moderate but selective activity against the P-388 tumor cell line with IC50 of 5 fig/mL. In addition, (-l-)-epiconicol, a marine metabolite, was isolated from Aplidium off. densum and exhibited... 

Kim, JJi. and Tannenbaum, S.R. (2004). S-Nitrosation regulates the activation of endogenous procaspase-9 in HT-29 human colon carcinoma cells. J. Biol. Chem. 279, 9758-9764. 

In cells lacking a functional p53 tumor suppressor protein, the endogenous free radical NO inhibits apoptosis and promotes growth of cancer cells. SNP or SNAP inhibited apoptosis in HT-29 human colon carcinoma cells initiated by flavone. NO effectively inhibits apoptosis by scavenging superoxide anions generated in the mitochondria of p53 mutant cells and thereby prevents the downregulation of the anti-apoptotic factor Bcl-X(L), which controls the mitochondrial apoptosis pathway (Wenzel et al. 2003). 

Matsushita, Y., Hoff, S. D., Nudelman, E. D., Ohtaka, M., Hakomori, S., Ota, D. M., Cleary, K. R., and Irimura, T., 1991, Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric LeX antigen, Clin. Exp. Metast. 9 283-299. 

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