High-pressure liquid chromatography analysis

Passarelli, R. J. and Jacobs, E. S., High-pressure liquid chromatography analysis of dyes and intermediates, ]. Chromatogr. Sci., 13, 153, 1975. 

Denaturing High Pressure Liquid Chromatography Analysis... 

HPLC High Pressure Liquid Chromatography. Analysis method for samples in liquid state. Used for Analysis of heavy crude oil fractions. 

The major application of porous, three-dimensional electrodes has been in metal removal from dilute process liquors (Chapter 7) although inorganic and organic dectrosynthesis applications continue to be explored (see, for example, the Dow-Huron cell for production in Chapter S). Many battery and l el cell electrodes utilize an active material which is (or is supported by) a porous, three-dimensional matrix (Chapter 11), while miniature porous electrodes have found use in electrochemical detector systems for high-pressure liquid chromatography analysis (Chapter 12). 

High-pressure liquid chromatography Analysis was performed by reverse-phase utilizing a Waters Associates chromatograph equipped with 30 cm X 4 mm i.d. micro Bondapak C g columns with 254-and 280-nm detectors at a flow rate of 60 ml/hr, using water-acetonitrile-acetic acid as a solvent system. 

High Pressure Liquid Chromatography. This modem version of the classical column chromatography technique is also used successfully for separation and quantitative analysis of dyes. It is generally faster than thin-layer or paper chromatography however, it requires considerably more expensive equipment. Visible and uv photometers or spectrophotometers are used to quantify the amounts of substances present. 

Using IR spectroscopy and NMR, one can analyze the chemical structure of PA. The molecular weight and molecular weight distribution can be analyzed by endgroup analysis, viscometry, and high-pressure liquid chromatography (HPLC). The crystalline order can be analyzed by WAXS, small-angle X-ray spectroscopy... 

Improved high-pressure liquid chromatography (HPLC) methods have been developed for the analysis of quaternary salt type corrosion inhibitors in brine waters [400]. However, these methods are not suitable for imidazolines and amido-amines. A method based on fluorescence detection has been described for the quantitative analysis of the imidazoline- and amido-amine-type corrosion inhibitors in both oil field water and crude oil samples by HPLC [1174]. 

Rastogi, S. C., Analysis of diisocyanate monomers in chemical products containing polyurethanes by high pressure liquid chromatography, Chro-matographia, 28, 15, 1989. 

Sample analysis was performed using a combination of Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC). 

The introduction and eventual commercialization of matrix-assisted laser desorption/ionization (MALDI) and electrospray (ESI) allowed biomarker status to be extended to proteins in 1996.15"17 With a few exceptions, ESI has been used in conjunction with extractions and high-pressure liquid chromatography (UPLC) interfaced with mass spectrometry. MALDI, on the other hand, has been widely adapted for rapid analysis of intact organisms, supported by bioinformatics.1819... 

The values of ks/kp for partitioning of carbocations are most conveniently determined as the ratio of the yields of products from the competing nucleophile addition and proton transfer reactions (equation 1 derived for Scheme 2). The determination of these product yields has been simplified in recent years by the application of high-pressure liquid chromatography (HPLC). Typically, the product peaks from an HPLC analysis are detected and quantified by UV-vis spectroscopy. In cases where the absorbance of reactants and products is small, substrates may be prepared with a chromophore placed at a sufficient distance so that its effects on the intrinsic reactivity of the carbocationic center are negligible. For example, the aliphatic substrates [1]-Y have proved to be very useful in studies of the reactions of the model tertiary carbocation [1+].21,23... 

Dunn, B.P. 1980. Polycyclic aromatic hydrocarbons in marine sediments, bivalves, and seaweeds analysis by high-pressure liquid chromatography. Pages 367-377 in A. Bjorseth and A.J. Dennis (eds.). Polynuclear Aromatic Hydrocarbons Chemistry and Biological Effects. Battelle Press, Columbus, OH. 

High-pressure gas separation, hollow-fiber membrane modules for, 15 823 High pressure liquid chromatography (hplc), 9 234 21 275 in herbicide analysis, 13 312 polymer analysis using, 19 566 High-pressure methanol, production process, 16 300-301 High pressure methods, specialized, 13 430-431... 

Antipyrine metabolism in vivo has also been demonstrated. Following extraction of media from uptake and elimination studies, TLC analysis revealed the parent compound and 4-hydroxy-antipyrine. Based upon the amount of radioactivity recovered, the metabolite may account for up to 4% of the total. This hy-droxylated metabolite is the primary oxidation product in animals studied to date (23). Further characterization of the extracts using high-pressure liquid chromatography will be done in the future. 

Holder, G., Yagi, H., Dansette, P., Jerina, D. M., Levin, W., Lu, A. Y. H., and Conney, A. H. Effects of inducers and epoxide hydrase on the metabolism of benzo(a)pyrene by liver microsomes and a reconstituted system Analysis by high pressure liquid chromatography. Proc. Natl. Acad. Sci. U.S.A. (1974) 71 4356-4360. 

A variety of methods are available for analyzing pharmaceutical compounds however, high-pressure liquid chromatography is currently the method of choice for the analysis of these compounds. High-pressure... 

This chapter presents an overview of current trends in high-pressure liquid chromatography (HPLC) instrumentation focusing on recent advances and features relevant to pharmaceutical analysis. Operating principles of HPLC modules (pump, detectors, autosampler) are discussed with future trends. 

Since its creation around 1973, modern high-pressure liquid chromatography (HPLC) has played a dominant role in the analysis of pharmaceuticals. It is used in many different applications for example, in content uniformity assays and stability-indicating methods, for the purity profiles of drug substances, or in the analysis of drug metabolism in animals and humans. The heart of all of these assays is the HPLC column. In this chapter, we will describe the fundamental properties of HPLC columns as well as how these properties influence column performance and separation characteristics in pharmaceutical assays. 

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