Ultra-high-pressure liquid chromatography analysis

The development of ultra high-pressure liquid chromatography technology has dramatically reduced the analysis time for a number of small molecules. However, we have found that the analysis of carotenes is poor with the currently available column chemistries. The development of C30 columns with backbones that can withstand ultra high pressure would allow carotenoid chemists to take full advantage of this technology. 

The use of LC with conventional LC columns (5 jm) is a common and well-established technique in food analysis. However, the limitations of the use of this type of column do not solve all analytical problems. This fact is especially critical in food metabolomics because of the complexity of food matrices and the high number of compounds of interest. Therefore, the use of ultra-high-pressure liquid chromatography (UHPLC) can overcome the limitations of conventional LC, providing... 

Ultra high performance liquid chromatography (UHPLC) or ultra performance liquid chromatography (UPLC) is a quite novel technique, but it is one of the most popular nowadays, due to the fact that it is much more rapid than conventional LC. UHPLC systems can work at very high pressures and are compatible with stationary phases with particle sizes of around two micrometers or even smaller. Thus, the development of UHPLC methods [69,77, 80] or the conversion of HPLC to UHPLC methods is a current trend, due mainly to the speed of analysis and low solvent use. 

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