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Fluorophores

Using guest fluorophores, they show that the adsorption energy increases linearly with the alkyl chain length as... [Pg.395]

Bhawalkar J D, Swiatkiewicz J, Pan S J, Samarabandu J K, Liou W S, He G S, Berezney R, Cheng P C and Prasad P N 1996 Three-dimensional laser scanning two-photon fluorescence confocal microscopy of polymer materials using a new, efficient upconverting fluorophore Scanning 18 562-6... [Pg.1675]

Carlsson Kand Liljeborg A 1997 Confocal fluorescence microscopy using spectral and lifetime information to simultaneously record four fluorophores with high channel separation J. Microsc. 185 37-46... [Pg.1675]

Mais S, Tittel J, Basche T and Brauchle C 1997 Terrylenediimide a novel fluorophore for single-molecule spectroscopy and microscopy from 1.4 K to room temperature J. Chem. Phys. A 101 8435-40... [Pg.2507]

Warshaw D M, Hayes E, Gaffney D, Lauzon A-M, Wu J, Kennedy G, Trybus K, Lowey S and Berger C 1998 Myosin conformational states determined by single fluorophore polarization Proc. Natl Acad. Sc/. USA 95 8034-9... [Pg.2511]

Tokunaga M, Kitamura K, Saito K, Iwane A H and Yanagida T 1997 Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy Biochem. Biophys. Res. Commun. 235 47-53... [Pg.2512]

C7>th e antigen, and I an indicator molecule such as an enzyme, fluorophore, or radioisotope. [Pg.22]

Excitation and emission wavelengths are for the unconjugated fluorophore. Wavelengths for conjugates may vary according to the composition of the conjugate. [Pg.27]

As the result of high specificity and sensitivity, nucleic acid probes are in direct competition with immunoassay for the analytes of some types of clinical analytes, such as infectious disease testing. Assays are being developed, however, that combine both probe and immunoassay technology. In such hybrid probe—immunoassays, the immunoassay portion detects and amplifies the specific binding of the probe to a nucleic acid. Either the probe per se or probe labeled with a specific compound is detected by the antibody, which in turn is labeled with an enzyme or fluorophore that serves as the basis for detection. [Pg.28]

In time-resolved fluorescence, rare earths are frequently used as fluorescent labels. The fluorophores have large Stokes shifts, ie, shifts of the emitted light to a higher wavelength relative to the absorption wavelength, and comparatively long decay times, approximately 0.5 ms. This simplifies the optical... [Pg.394]

Nevertheless, quantification is possible, a good example being the evaluation of the composition of chromatographic separations adsorbed onto glass, alumina, polyethylene, or paper. When compared with known standards, the presence of only a few nanograms of a strong fluorophore may be quantified to better than 10%. [Pg.382]

Structure of GFP and its chromophore. To study the chro-mophore of GFP, a sample of GFP was denatured by heating it at 90°C. It was digested with papain, and then a peptide containing the fluorophore was isolated and purified from the digested mixture. The structural study of the peptide has indicated that the chromophore of GFP is an imidazolone derivative shown below (Shimomura, 1979). This chromophore structure was confirmed later by Cody etal. (1993) in a hexapeptide isolated from GFP. It is intriguing that the structure of the GFP chromophore is a part of the structure of coelenterazine. [Pg.131]

Nitroxides have the property of quenching fluorescence. Thus radical trapping with nitroxides containing fluorophores (e.g. 114) can be monitored by observing the appearance of fluorescence.511015 The method is highly sensitive and has been applied to quantitatively determine radical yields in PLP experiments (Section... [Pg.139]

Jablonski (48-49) developed a theory in 1935 in which he presented the now standard Jablonski diagram" of singlet and triplet state energy levels that is used to explain excitation and emission processes in luminescence. He also related the fluorescence lifetimes of the perpendicular and parallel polarization components of emission to the fluorophore emission lifetime and rate of rotation. In the same year, Szymanowski (50) measured apparent lifetimes for the perpendicular and parallel polarization components of fluorescein in viscous solutions with a phase fluorometer. It was shown later by Spencer and Weber (51) that phase shift methods do not give correct values for polarized lifetimes because the theory does not include the dependence on modulation frequency. [Pg.9]

By using phase sensitive detection, the detector phase angle can be adjusted to be exactly out of phase with the phase-delayed emission from any single fluorophore, suppressing its contribution to the total emission signal. Phase sensitive detection, coupled with... [Pg.10]

Membrane depolarization can be measured by members of a class of fluorophores (commonly referred to as the carbocyanine dyes) which have been designed to partition into the membrane, where their orientation and spectral properties change with changes in the electrochemical gradient across the membrane (18). 3,3 -dipropyl-... [Pg.26]


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Aminonaphthalimide fluorophore

Anisotropy of Planar Fluorophores with High Symmetry

Anthracene fluorophore

Antibodies optimal fluorophore

Antibodies, fluorophore-labeled

BODIPY fluorophore

BODIPY fluorophores

BODIPY fluorophores fluorescent quenching

BODIPY fluorophores properties

Biarsenical fluorophores

Biochemical Fluorophores

Biotin-fluorophores

Calcium fluorophores

Calcium sensitive fluorophore

Cascade Blue fluorophores

Complexity, Measurement Scheme, and the Spectral Properties of Fluorophores

Coumarin fluorophores

Coumarin-type fluorophore

Cyclodextrin fluorophore

Dansyl fluorophore

Depolarization fluorophore rotation

Dextran fluorophor- labeled

Dual fluorophore-nitroxide molecules

Effect of Fluorophore Labeling

Emission by Fluorophores near a Surface

Environment-sensitive fluorophores

Excitation Photoselection of Fluorophores

Excitation-emission fluorescence fluorophores

Extinction coefficient fluorophores

Extrinsinc fluorophores

Fluorescein, fluorophore

Fluorescence fluorophores

Fluorescence microscopy fluorophore choice

Fluorescence microscopy fluorophore selection

Fluorescence multi-fluorophore solution

Fluorescence staining fluorophore choice

Fluorescent labeling, generations fluorophores

Fluorophore

Fluorophore Characterization and Importance in Biology

Fluorophore LM-1 (

Fluorophore Mixtures

Fluorophore absorption band, metallic

Fluorophore brightness

Fluorophore browning

Fluorophore classification

Fluorophore conjugated streptavidin

Fluorophore decay characteristics

Fluorophore decay time

Fluorophore dependence in modular systems

Fluorophore distance dependence

Fluorophore effect

Fluorophore enhanced emission

Fluorophore environment

Fluorophore intracellular

Fluorophore isothiocyanates

Fluorophore label

Fluorophore labeling

Fluorophore lifetimes

Fluorophore luminescent label

Fluorophore maturation

Fluorophore peak analysis

Fluorophore photobleaching

Fluorophore photostability

Fluorophore probes, microscopy studies

Fluorophore properties

Fluorophore properties coupling

Fluorophore properties enhancement

Fluorophore properties fluorescence detection

Fluorophore properties metal-enhanced fluorescence

Fluorophore quenching

Fluorophore rotational freedom

Fluorophore saturation

Fluorophore selection

Fluorophore selection long-wavelength fluorophores

Fluorophore selection microscopy

Fluorophore sensitivity

Fluorophore structure

Fluorophore-assisted

Fluorophore-assisted carbohydrate

Fluorophore-assisted carbohydrate electrophoresis

Fluorophore-assisted carbohydrate electrophoresis FACE)

Fluorophore-coupled

Fluorophore-forming reactions

Fluorophore-metal composites

Fluorophore-metallic surface

Fluorophore-metallic surface interactions

Fluorophore-metallic surface theory

Fluorophore-nanostructure combinations

Fluorophore-spacer-receptor

Fluorophores BODIPY derivatives

Fluorophores FRET pair

Fluorophores and quenchers

Fluorophores binding site

Fluorophores brightness

Fluorophores catalytic production

Fluorophores characteristics

Fluorophores coumarin derivatives

Fluorophores covalent modification

Fluorophores cyanine dyes

Fluorophores cyanines

Fluorophores electron energy levels

Fluorophores encapsulation into liposomes

Fluorophores energy transfer

Fluorophores extrinsic

Fluorophores extrinsic probes

Fluorophores families

Fluorophores feature

Fluorophores fluorescein derivatives

Fluorophores fluorescence properties

Fluorophores fluorescent ligands

Fluorophores fluorescent molecules, synthesis

Fluorophores fluorescent properties

Fluorophores fluoroscein

Fluorophores general characteristics

Fluorophores general structure

Fluorophores immunoassays

Fluorophores infrared

Fluorophores intrinsic

Fluorophores labeling between nucleotides

Fluorophores lanthanide complexes

Fluorophores lifetimes

Fluorophores modified base

Fluorophores molecular beacon reporter

Fluorophores molecular rotors

Fluorophores near-infrared

Fluorophores optical sensors

Fluorophores oxazines

Fluorophores photodestruction

Fluorophores photophysical effects

Fluorophores polarity affects

Fluorophores protein intrinsic fluorescence

Fluorophores proteins

Fluorophores pyrene

Fluorophores quantum yield

Fluorophores quenchers properties

Fluorophores quenchers, structures

Fluorophores resonance interactions

Fluorophores rhodamine derivatives

Fluorophores terminal labeling

Fluorophores visible

Fluorophores, pendant, complexes

Fluorophors

Forster resonance energy transfer fluorophores

Functional monomers fluorophores

Harvester fluorophore

Hypervariable region effect on fluorophore

Imprinting of silicas with fluorophores

Interaction fluorophore-metal

Intrinsic chromophores and fluorophores

Labeling with BODIPY fluorophores

Lanthanide fluorophores

Lanthanide fluorophores labelling

Lipophilic fluorophores

Lucifer Yellow fluorophores

Magnesium -selective fluorophores

Metallic nanoparticles fluorophore properties

N6-Ethenopurines as Fluorophores

Nanoparticle-fluorophore distance, metal

Naphthalene fluorophore

Nucleic acid fluorophor labeling

Organic fluorophore

Organic fluorophores

Other Infrared Fluorophores

Oxazoles fluorophores

Particle-fluorophore interactions, gold

Partitioning and Binding of Fluorophore Quenchers to Membranes

Phenanthroline fluorophore

Photo-excited fluorophore

Photoactivatable fluorophore

Polymer fluorophores

Proteins fluorophore labeling

Proteins labelling with fluorophores

Quantum dots fluorophore labels

Quantum yield BODIPY fluorophores

Quantum yield Lucifer Yellow fluorophores

Quantum yield common fluorophores

Quantum yield, fluorophore

Quenching particle-fluorophore interactions

Quinine, fluorophore

Reorientation of Dipoles in the Fluorophore Environment

Ruthenium complex with fluorophore

Silver island films fluorophores

Small-molecule fluorophore

Stokes shift fluorophores

Streptavidin-fluorophore

Studies of Polymers Carrying Medium-Sensitive Fluorophores

Sugar-amine fluorophore, chemical

Support for Fluorophores

Surface Distribution of Fluorophore-Labeled Lipids

TAMRA fluorophore

TAMRA fluorophores

TP fluorophores

Time Resolved Spectroscopy of Fluorophores Bound to Metal Nanoparticles

Types of fluorophores

Yeast fluorophore labeling

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