Fluorophore-assisted carbohydrate

To assess the use of HPLC and fluorophore assisted carbohydrate gel electrophoresis as a means of profiling caramel colour types III and IV. 

When HA samples contain only several disaccharide repeating units (from 10- to 12-mers), PAGE is not appropriate. An alternative gel electrophoretic procedure known as Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) is more suited for these cases. In FACE technique, the sample is derivatized with a fluorescent group at the reducing end-group prior to electrophoresis. The FACE electrophoretic method was also described in detail by Cowman and Mendichi [270]. 

Calabro, A. et al. Fluorophore-assisted carbohydrate electrophoresis, FACE of glycosaminoglycans. Osteoarthritis Cartilage, 9 (SupplA), SI6, 2001. 

MoreU, M. K., Samnel, M. S. and O Shea, M. G Analysis of starch structure using fluorophore-assisted carbohydrate electrophoresis. 19, 2603, 1998. 

Guo-Fu H (1995) Fluorophore-assisted carbohydrate electrophoresis technology and applications. Journal of Chromatography A 705 89-103. 

S. S. Basu, D. Dastgheibhosseini, G. Hoover, Z. X. Li, and S. Basu, Analysis of glycosphingolipids by fluorophore-assisted carbohydrate electrophoresis using ceramideglycanase from Afercenar/amercenana, Anal. Biochem. 222 270-274 (1994). 

Robbe, C. Capon, C. Flahaut, C. Michalski, J.-C. Microscale analysis of mucin-type O-glycans by a coordinated fluorophore-assisted carbohydrate electrophoresis and mass spectrometry approach. Electrophoresis 2003, 24, 611-621. 

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