Spectroscopy single molecule

we never experiment with just one electron or atom or (small) molecule. In thought experiments we sometimes assume that we do this invariably entails ridiculous consequences... E. Schrodinger, Br. J. Philos. Sci. 1952, 3, 233-242. [Pg.133]

Fluorescence correlation spectroscopy analyses the temporal fluctuations of the fluorescence intensity by means of an autocorrelation function from which translational and rotational diffusion coefficients, flow rates and rate constants of chemical processes of single molecules can be determined. For example, the dynamics of complex formation between /3-cyclodextrin as a host for guest molecules was investigated with singlemolecule sensitivity, which revealed that the formation of an encounter complex is followed by a unimolecular inclusion reaction as the rate-limiting step.263 [Pg.134]

Derive Equation 3.25 using the hint given in the footnote to that equation. [Pg.134]

A plot of the inverse quantum yield for the photocycloaddition of 1-aminoanthraqui-nones (AQ) and (E)-piperylene (Q) was found to be linear and the ratio of the slope, 0.20 dm3 mol and intercept, 1.20, of this plot was equal to the Stern Volmer constant for fluorescence quenching of AQ by Q, Ksv = 0.17 dm3 mol It was concluded that the reaction proceeds from the excited singlet state of AQ via an exciplex intermediate (AQ-Q). Explain. [Ref. 270] [Pg.136]

The fluorescence quantum yield of anthracene in degassed acetonitrile is 0.3. It is reduced by 30% upon admission of air. Estimate the lifetime of the singlet state of anthracene in degassed solution and the rate constant of fluorescence. Refer to Section 2.2.1. The concentration of dioxygen in air-saturated acetonitrile is 2x10 3m. [tj = 5.6 ns, kf = 5.4 x 107 s ] [Pg.136]

Fluorescence and vibrational microscopy with conventional spectrometers and microscopes can provide only as much molecular detail as cdlowed by the diffraction limit. Most molecules—including biopolymers—have dimensions that are much smaller than visible wavelengths, so special techniques had to be developed to visualize single molecules with optical microscopes. Here we outline the most popular strategies comprising a collection of tools known as single-molecule spectroscopy. [Pg.493]

In far-field confocal microscopy, laser light focused by an objective lens is used to illuminate about 1 pm of a very dilute sample placed beyond the near field. This illumination scheme is limited by diffraction and, as a result, data from far-field microscopy have less structural detail than data from NSOM. However, far-field microscopes are very easy to construct and the technique can be used to probe single molecules as long as there is one molecule, on average, in the illuminated area. [Pg.493]

In the wide-field epifluorescence method, a CCD detects fluorescence excited by a laser and scattered back from the sample (Fig. 12.47a). If the fluorescing molecules are well separated in the specimen, then it is possible to obtain a map of the distribution of fluorescent molecules in the illuminated area. For example. Fig. 12.47b shows how epifluorescence microscopy can be used [Pg.493]

Many of the most interesting current developments in electronic spectroscopy are addressed in special chapters of their own in this encyclopedia. The reader is referred especially to sections B2.1 on ultrafast spectroscopy. Cl.5 on single molecule spectroscopy, C3.2 on electron transfer, and C3.3 on energy transfer. Additional topics on electronic spectroscopy will also be found in many other chapters. [Pg.1147]

Xie X S 1996 Single-molecule spectroscopy and dynamics at room temperature Acc. Chem. Res. 29 598-606... [Pg.1799]

The teclmiques and applications of single molecule spectroscopy are currently in a state of rapid development, making tliis a difficult field to summarize at any given time. This contribution is, at best, a single frame of a movie... [Pg.2503]

Durand Y, Woehl J C, Viellerobe B, Gdhde W and Orrit M 1999 New design of a cryostat-mounted scanning near-field optical microscope for single molecule spectroscopy Rev. Sc/. Instrum. 70 1318-25... [Pg.2505]

Ha T, Chemla D S, Enderle T and Weiss S 1997 Single molecule spectroscopy with automated positioning Appl. [Pg.2505]

Plakhotnik T, Donley E A and Wild U P 1997 Single-molecule spectroscopy Ann. Rev. Phys. Chem. 48 181-212... [Pg.2506]

Kummer S, Basche T and Brauchle C 1994 Terrylene in p-terphenyl a novel single crystalline system for single molecule spectroscopy at low temperatures Chem. Phys. Lett. 229 309-16... [Pg.2506]

Piakhotnik T, Moerner W E, Irngartinger T and Wild U P 1994 Single molecule spectroscopy in Shpol skii matrices Chimia 48 31-2... [Pg.2507]

Pirotta M, Renn A, Werts M FI V and Wild U P 1996 Single molecule spectroscopy. Perylene in the Shpol skii matrix n-nonane Chem. Phys. Lett. 250 576-82... [Pg.2507]

Mais S, Tittel J, Basche T and Brauchle C 1997 Terrylenediimide a novel fluorophore for single-molecule spectroscopy and microscopy from 1.4 K to room temperature J. Chem. Phys. A 101 8435-40... [Pg.2507]

Tittel J, Kettner R, Basche T, Brauchle C, Quante FI and Mullen K 1995 Spectral diffusion in an amorphous polymer probed by single molecule spectroscopy J. Lumin. 64 1-11... [Pg.2507]

Zilker S J, Kador L, Friebel J, Vainer Y G, Kol chenko M A and Personov R I 1998 Comparison of photon echo, hole burning, and single molecule spectroscopy data on low-temperature dynamics of organic amorphous solids J. Phys. Chem 109 6780-90... [Pg.2507]

Wild U P, Guttler F, Pirotta M and Renn A 1992 Single molecule spectroscopy stark effect of pentacene in p-terphenyl Chem. Phys. Lett. 193 451-5... [Pg.2508]

Guttler F, Sepiol J, Plakhotnik T, Mitterdorfer A, Renn A and Wild U P 1993 Single molecule spectroscopy fluorescence excitation spectra with polarized light J. Lumin. 56 29-38... [Pg.2508]

Bach FI, Renn A, Zumofen G and Wild U P 1999 Exciton dynamics probed by single molecule spectroscopy Phys. Rev. Lett. 82 2195-8... [Pg.2508]

Janshoff, A., Neitzerl, M., Oberdorfer, Y. and Fuchs, H., Force spectroscopy of molecular systems - single molecule spectroscopy of polymers and biomolecules. Angew. Chem. Int. Edn., 39(18), 3213-3237 (2000). [Pg.216]

Pettinger, B., Picardi, G., Schuster, R. and Ertl, G. (2000) Surface enhanced Raman spectroscopy towards single molecule spectroscopy. Electrochemistry, 68, 942-949. [Pg.17]

There are two important drawbacks of such an approach (1) a polarity scale based on a particular class of probes, in principle, does not account, for example, sizes of probes, which should strongly effect the interactions (2) betain dyes do not fluoresce, which restrict essentially the field of application of this approach, because in many cases, absorption spectrum could not be measured accurately (small volumes of samples, study of cells, and single molecules spectroscopy). Therefore, polarity-sensitive fluorescent dyes offer distinct advantage in many applications. [Pg.208]

Dye stuffs are among the oldest topics of synthetic organic and industrial chemistry, and it is exciting to follow their role in nanosciences and single molecule spectroscopy. [Pg.326]

Optical properties of dendrimers bearing eight chromophores have been examined by single molecule spectroscopy techniques. It is especially exciting that variations in the spectra are recorded if one of these dendrimers is observed for a period of time under continuous irradiation.1481 The fluorescence intensity of the dendrimer nanoparticle also jumps between discrete emissive levels. All these findings suggest the existence of strong electronic interactions between several perylene imide chromophores within one dendrimer and provide new... [Pg.334]

Single-molecule spectroscopy of a dendrimer-based host-guest system. Chem Eur J 7 4126-4133... [Pg.189]

Schlindler F, Lupton JM, Feldmann J, Scherf U (2004) A universal picture of chromophores in 7i-conjugated polymers derived from single-molecule spectroscopy. Proc Natl Acad Sci 101 14695-147000... [Pg.388]

The attachment of a distinct number of fluorescent dyes at the periphery of a stiff three-dimensional nanoparticle is of fundamental interest to study interactions between single chromophores in close vicinity to each other. We chose as a dye one of the rylene series, perylenemonoimide, for the decoration of our polyphenylene dendrimers due to their outstanding properties. Rylene dyes show an exceptional chemical and photochemical stability and therefore are well suited for single molecule spectroscopy (SMS) [661. [Pg.29]

The observed unidirectional (no reversal of rotation) and discrete rotation of the y subunit in steps of 120° during ATP hydrolysis by single molecule spectroscopy [34,35,40] supports the irreversible mode of operation of a single enzyme molecule that has been envisaged for the formulation of the thermody-... [Pg.83]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...