Coumarin-type fluorophore

In order to quantify the physical environment of a bioreactor, fluorescence assays can be applied for on-line monitoring of the mixing time behavior of all types of bioreactors. In this case the fluorosensor probe can be installed in the bioreactor at different locations of interest. Afterwards, selected fluorophores can be injected in order to study the overall mixing time. These fluorophores must fit to the excitation and emission behavior of the probe and should be selected in regard to the pH-dependency of the bioprocess, and when used during cell cultivation experiments they should not interfere with the cells. Scheper and Schiigerl reported on the use of different coumarins for mixing time experiments under bioprocess relevant conditions [49]. 

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