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Exfoliated cells

After repeated wash and centrifugation cycles, cells were spin-deposited via cytocentrifugation onto low-e slides (see later). Uniform, sparse samples of cells, which adhere to the substrate very strongly, could be produced this way. [Pg.183]

The two major cell types identified in the urine were urothelial cells (from the lining of the bladder) and mature squamous cells (from the distal end of the urethra). The presence or absence of glycogen in both cell types can be demonstrated spectroscopically. [Pg.176]

The cervical cells were obtained via a routine gynecological examination, and stored on the collection devices in fixative until a satisfactory cytological reading was established (these samples otherwise would have been discarded). Consent to participate in this project was obtained from each patient. In aU of the studies reported below, storage of the fixative was shown not to cause any spectral degeneration of the cells. [Pg.176]


For the analysis of exfoliated cells (such as an automated Pap test for cervical cancer screening), the ability to collect the spectrum of each individual cell separately by mapping methods is even more important, since statistical analysis... [Pg.189]

Cytology, that is, the visual inspection of exfoliated cells, is most commonly carried out for smears of cells, which are deposited ( smeared ) directly from brushes, spatulas or other exfoliation devices onto microscope slides. Such smears are unsuitable for spectral analysis, since they contain clumps of cells, cellular debris, erythrocytes and other contamination. However, better methods of cell slide preparations have been introduced into cytology, among them the ThinPrep methods developed by Cytyc, Inc. (see ref. 7), and spin centrifugation deposition techniques. These methods are very good for real exfoliated cell samples,7 since they permit the purification of the cell exfoliate, enrichment in the cells desired for analysis and produce good monolayers for visual cell inspection. [Pg.192]

Figure 17. An example using the scan and count technique to count exfoliative cells in a Pap smear see text for description. Figure 17. An example using the scan and count technique to count exfoliative cells in a Pap smear see text for description.
Ai 1 h after exposure, injury was difficult to assess because the trachea] surfaces were coveted with exfoliated cells or were in total disarray. At 6 h, lesions were well defined and Luge flattened cells covered the basement membranes where mucosal cells had exfoliated. [Pg.296]

Biomonitoring for human health hazard surveillance typically involves collection of hair, expired air, bodily fluids (blood, urine, saliva, breast milk, semen), feces, epithelial scrapings, exfoliated cells, or, less frequently, tissue biopsies from people known or suspected of being exposed to potential chemical, physical, or biological hazards. These samples are analyzed for toxic chemicals, their metabolites, or biomarker responses. The most commonly used biomarkers in humans are those relating to DNA and chromosomal damage, because these effects contribute to cancer risk. Besides biomarkers of chemical exposure and effects, there are also human biomarkers of tumor formation and susceptibility to toxic... [Pg.296]

The detection of telomerase activity or hTERT mRNA in excretion, secretion, brushings, and washings has been evaluated. In pancreatic, secretion samples, which contain freshly exfoliated cells, detection of telomerase activity was encouraging for cancer detection. Telomerase activity was also found to be useful in distinguishing between adenoma and carcinoma in intraductal papillary-mucinous tumors, which can sometimes be difficult to diagnose. In bronchial brushing samples, the clinical sensitivity of cancer detection is below 70% and false-positive results are commonly encountered because of contamination witli lymphocytes, which contain measurable telomerase activity. The measurement of telomerase is also expected to become a useful... [Pg.764]

Wong, P.T.T., Lacelle, S., Sentermann, M. and Fung, F.K.M. (1995) Characterization of the exfoliated cells and tissues from the human endo- and ecto-cervix by FT-IR and ATR-FT-IR spectroscopy. Biospectroscopy, 1, 357-64. [Pg.146]

NC, USA) and Cytyc (Marlboro, MA, USA). When several different spectral cytology fixatives were tested, the fixation of individual exfoliated cells was shown to cause relatively small spectral changes, in contrast to earlier reports where there was a heavy dependence on fixation [14]. (These results are presented in Section 5.3.1.) As the present cytology procedures involved use of the SurePath method, a SurePath preservative fiuid was used as fixative this was an aqueous solution containing ca. 25% ethanol and a few percent of isopropanol and methanol. [Pg.177]

This section contains spectral results collected from entire individual cells, with the specific aim of differentiating between cell types that occur in samples of cells. The long-range goal of these studies has been the development of a completely automatic method to perform spectral cytology-that is, to analyze a sample of exfoliated cells (e.g., cervical cells obtained during the Pap cervical cancer screening procedure) for the presence of abnormal cells. [Pg.184]

Oral mucosa (buccal) cells have been used as one of the most easily obtainable exfoliated cell types from volunteer donors. The spectra collected from entire individual cells are used to introduce the possibiUty of using IR microspectral results to distinguish between ceU types and to determine their state of health. In particular, the heterogeneity of observed spectra-and methods to overcome such problems-are discussed. [Pg.184]

Promoters and carcinogens involved in human cancer induction were first discovered through carefully conducted epidemiological studies, and then tested in animal models or in in vitro systems. Newer techniques use exfoliated cells or cells in culture to ascertain exposure to these genotoxic materials. ... [Pg.262]

Saccomanno G, Archer V, Auerbach 0, et al. 1974. Development of carcinoma of the lung as reflected in exfoliated cells. Cancer 33 256-270. [Pg.124]

Ovarian cancer spreads primarilyby direct extension to neighboring organs by exfoliating cells into flie peri-... [Pg.240]

Presant and Bearman (1981) reported preliminary findings of a randomized trial that used isotretinoin either alone or in combination with busulfan for the treatment of patients with chronic phase chronic myelocytic leukemia. No differences have been noted in the time to development of blastic transformation in either group. The addition of the retinoid did not increase the acute toxicity of busulfan. Saccomanno et al. (1982) administered isotretinoin in 26 patients who exhibited varying degrees of cytologic atypia in exfoliated cells obtained from... [Pg.360]

Rothacker, J., Ramsay, R.G., Ciznadija, D., Gras, E., Neylon, C.B., Elwood, N.J., Bouchier-Hayes, D., Gibbs, P., Rosenthal, M.A., Nice, E.C., 2007. A novel magnetic bead-based assay with high sensitivity and selectivity for analysis of telomerase in exfoliated cells from patients with bladder and colon cancer. Electrophoresis 28,4435 1446. [Pg.200]

Sample collection may be noninvasive (e.g., urine, feces, sputum, collection of exfoliated cells with buccal swab) or invasive (e.g., blood taking and biopsy). The measured parameter may show time dependence in such cases the sample should be taken at various intervals and the time course of the parameter (such as drug clearance) should be determined. Biological fluids are (in most cases) homogenous. For nonhomogenous samples (such as tissues), it is important to estabhsh that the sample taken is representative and for all persons involved in the study the same type (or same fraction) of sample is studied. [Pg.26]


See other pages where Exfoliated cells is mentioned: [Pg.247]    [Pg.247]    [Pg.486]    [Pg.277]    [Pg.215]    [Pg.443]    [Pg.205]    [Pg.236]    [Pg.225]    [Pg.229]    [Pg.190]    [Pg.130]    [Pg.1341]    [Pg.1343]    [Pg.375]    [Pg.173]    [Pg.175]    [Pg.175]    [Pg.177]    [Pg.178]    [Pg.189]    [Pg.1954]    [Pg.484]    [Pg.67]    [Pg.890]    [Pg.191]    [Pg.172]    [Pg.7]    [Pg.48]    [Pg.277]    [Pg.16]    [Pg.139]    [Pg.183]   
See also in sourсe #XX -- [ Pg.183 , Pg.195 , Pg.196 , Pg.197 ]




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Exfoliants

Exfoliate

Exfoliating

Exfoliation

Exfoliators

Fixation Studies of Exfoliated Cells

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