Slide preparation

The same generally impervious properties make spores difficult to stain by simple stains. However, if a slide preparation of spores is warmed with a stain the spores are dyed so effectively that dilute acid will not wash out the colour. This is the basis of the acid-fast stain for spores. 

Remove the fixative bath vial with the prepared slide from its holder when the slide preparation process is complete. 

GYNECOLOGIC SPECIMENS THINPREP SLIDE PREPARATION Principle... 

Peripheral blood cultures are established in medium containing BrdU and PHA. Cocemid is added 1-2 h before harvest and the cells are harvested between 60 and 70 h post-PHA stimulation. Cell harvest and slide preparations are conducted according to routine cytogenetic methods. 

The slide preparation is fixed by using either precipitating fixatives such as ethanokacetic acid or cross-linking fixatives such as paraformaldehyde. They are further treated to remove protein with a mixture that includes proteinase K. Removal of proteins facilitates the access of probe to the sample DNA target. 

The acylation and amination reactions to prepare the dendrimer start with the amine slides prepared according to the above procedure. 

As can be seen from the previous slides, preparation for accreditation is a tremendous amount of work. Nevertheless it will be increasingly necessary for laboratories, especially if they want to be active in different countries. 

Slides prepared in this way can be stored indefinitely at room temperature. 

Cut 5-pm sections from routine paraffin embedded blocks onto slides prepared as above... 

This and the following methods sections apply to slides prepared by any of the three methods described above... 

Cytology, that is, the visual inspection of exfoliated cells, is most commonly carried out for smears of cells, which are deposited ( smeared ) directly from brushes, spatulas or other exfoliation devices onto microscope slides. Such smears are unsuitable for spectral analysis, since they contain clumps of cells, cellular debris, erythrocytes and other contamination. However, better methods of cell slide preparations have been introduced into cytology, among them the ThinPrep methods developed by Cytyc, Inc. (see ref. 7), and spin centrifugation deposition techniques. These methods are very good for real exfoliated cell samples,7 since they permit the purification of the cell exfoliate, enrichment in the cells desired for analysis and produce good monolayers for visual cell inspection. 

The slide surface has to be planar, uniform, inert and accessible. Several materials can provide slides with these characteristics, but the glass surface is the most commonly used for fluorescent labeling, since most plastics do not permit the use of fluorescent dyes. Different glasses are available that are suitable for slide preparation (borosilicate, fused silica, etc.) and all are stable materials with low intrinsic fluorescence and reflectivity and an efficient transmission throughout the visible range. In order to allow the efficient... 

Fig. 4 Slide preparation and probe immobilization scheme. A clean slide is treated with mercaptosilane (MPTS), and an intermediate mercaptosilane layer is formed on the surface of the slide. 5 disulfide-modified probes are immobilized onto the slide through thiol/disulfide exchange reactions...

Self-contained fan Variable (user) slide preparation... 

Legator, M.S. Palmer, K.A., and Adler, I-D. 1973 A collaborative study of in vivo cytogenetic analysis. 1. Interpretation of slide preparations. Toxicol, and Applied. Pharm., 24 337-350. 

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