Epidermal growth factor cell culture

Nakaji-Hirabayashi T, Kato K, Arima Y, Iwata H (2007) Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells. Biomaterials 28 3517-3529... 

LOX-dependent superoxide production was also registered under ex vivo conditions [55]. It has been shown that the intravenous administration of lipopolysaccharide to rats stimulated superoxide production by alveolar and peritoneal macrophages. O Donnell and Azzi [56] proposed that a relatively high rate of superoxide production by cultured human fibroblasts in the presence of NADH was relevant to 15-LOX-catalyzed oxidation of unsaturated acids and was independent of NADPH oxidase, prostaglandin H synthase, xanthine oxidase, and cytochrome P-450 activation or mitochondrial respiration. LOX might also be involved in the superoxide production by epidermal growth factor-stimulated pheochromo-cytoma cells [57]. 

Brock R, Hamelers IHL, Jovin TM. Comparison of fixation protocols for adherent cultured cells applied to a GFP fusion protein of the epidermal growth factor receptor. Cytometry 1999 35 353-362. 

Baguley BC, Marshall ES, Holdaway KM, et al. Inhibition of growth of primary human tumour cell cultures by a 4-anilinoquinazoline inhibitor of the epidermal growth factor receptor family of tyrosine kinases. EurJ Cancer 1998 34 1086-1090. 

Epidermal growth factor (EGF) Many cultured cells, cells of skin and eyes... 

This study reports the first isolation and characterization of neural progenitor and stem cells from the adult brain. Neural progenitor and stem cells were isolated from mouse striatal tissue containing the subventricular zone (a neurogeneic area of the adult brain) and cultured in vitro in the presence of epidermal growth factor. 

Figure 1.11 Growth curves of a mixture of astrocytes and oligodendrocytes from C-6 rat glioma 2B cells the cells were incubated for 14 days with or without EGF (epidermal growth factor). EGF was added to the medium in three increasing doses. By 10 days, the cell cultured with EGF showed a statistically significant faster growth than the controls without EGF. By day 14, growth rates in all experimental cells had declined to control values.47 (From Timiras, PS. et al., Mech. Ageing Dev., 126, 3, 2005. With permission.)...

Jin, K., Mao, X. O., Del Rio Guerra, G., Jin, L., and Greenberg, D. A. (2005). Heparin-binding epidermal growth factor-like growth factor stimulates cell proliferation in cerebral cortical cultures through phosphatidylinositol S kinase and mitogen-activated protein kinase. J. Neurosci. Res. 81, 497-505. 

Greuet J, Pichard L, Ourlin JC, et al. Effect of cell density and epidermal growth factor on the inducible expression of CYP3A and CYP1A genes in human hepatocytes in primary culture. Hepatology 1997 25 1166-1175. 

Schroder and co-workers (Schroder etal., 1999,2000) studied PolyP metabolism in bone tissues and osteblast cultures. They revealed that PolyP metabolism in human osteoblasts was modulated by stimulators of osteoblast proliferation and differentiation (Leyhausen et al., 1998). A combined treatment of the cells with dexamethasone, ft-glycerophosphate, epidermal growth factor (EGF), and ascorbic acid resulted in a dramatic decrease in PolyP content. This decrease is caused mainly by a decrease in the amount of soluble long-chain PolyPs. The amount of this PolyP fraction, but not the amount of insoluble long-chain PolyPs, further decreases after additional treatment of the cells with la, 25-dihydroxy vitamin D3. The decrease in PolyP content during treatment with dexamethasone, ft-glycerophosphate, EGF and ascorbic acid is accompanied by a decrease in exopolyphosphatase activity. However, additional treatment with la, 25-dihydroxyvitamin D3 results in a significant increase of the enzyme activity. Therefore, it is reasonable to assume that PolyP... 

Endothelial cells are maintained in a basal culture medium, such as DMEM or a proprietary medium supplied with the cells, which is supplemented with hydrocortisone (lug/mL). epidermal growth factor (EGF 100 ug/mL) bovine fibroblast growth factor (FGF 1 ng/mL), antibiotics (gentamicin and amphotericin, at 50 ug/mL) and fetal calf serum (2-10%). Alternatively, bovine brain extract (3 ug/mL) can be used instead of EGF and FGF. The cells are cultured in either standard tissue culture flasks directly on plastic or on flasks coated with collagen. The cells are grown to confluence and subcultured and reseeded in a ratio of 1 3. For all experiments primary endothelial cells should be used between passages 3 and 12. 

L14. Lui X. H., Wiley, H. S., and Meikle, A. W., Androgens regulate proliferation of human prostate cancer cells in culture by increasing transforming growth factor-a (TGF-a) and epidermal growth factor (EGF)/TGF-a receptor. J. Clin. Endocrinol. Metab. 77, 1472—1478 (1993). 

It has been reported that vanadate(V) enhances the stimulatory effects of insulin on DNA synthesis in cultured mammary gland cells of mice103 . Vanadate(V) in the absence of insulin has little effect. Insulin and vanadate(V) act as comitogens. The reported experiments do not preclude the possibility that vanadyl(IV) is the form of vanadium responsible for these effects. Vanadate(V) has also been reported to be an epidermal growth factor stimulating DNA synthesis104. ... 

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