Osteoblasts proliferation

Z.M. Isa, G.B. Schneider, R. Zaharias, D. Seabold, C.M. Stanford, Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression, Int. J. Oral Maxillofac. Implants 21 (2006) 203-211. 

Elias et al. [21] CNT-containing orthopedic materials Osteoblasts - inoculation on material Increase of osteoblast proliferation, increase of alkaline phosphatase activity, absence of cytotoxicity... 

Supronowicz et al. [22] PLA/CNT - nano-composites Osteoblasts - contact with nanocomposites, effect of electric current Increase of osteoblast proliferation... 

Damien E, Price JS, Lanyon LE. Mechanical strain stimulates osteoblast proliferation through the estrogen receptor in males as well as females. J Bone Miner Res. 2000 15 2169-2177. 

Schroder and co-workers (Schroder etal., 1999,2000) studied PolyP metabolism in bone tissues and osteblast cultures. They revealed that PolyP metabolism in human osteoblasts was modulated by stimulators of osteoblast proliferation and differentiation (Leyhausen et al., 1998). A combined treatment of the cells with dexamethasone, ft-glycerophosphate, epidermal growth factor (EGF), and ascorbic acid resulted in a dramatic decrease in PolyP content. This decrease is caused mainly by a decrease in the amount of soluble long-chain PolyPs. The amount of this PolyP fraction, but not the amount of insoluble long-chain PolyPs, further decreases after additional treatment of the cells with la, 25-dihydroxy vitamin D3. The decrease in PolyP content during treatment with dexamethasone, ft-glycerophosphate, EGF and ascorbic acid is accompanied by a decrease in exopolyphosphatase activity. However, additional treatment with la, 25-dihydroxyvitamin D3 results in a significant increase of the enzyme activity. Therefore, it is reasonable to assume that PolyP... 

Jonsson KB. A new fluorometric assay for determination of osteoblastic proliferation Effects of glucocorticoids and insulin-like growth factor-I. Calcif Tissue Int 1997 60 30-6. 

Many cells secrete at least one of the three immature forms of TGF-P, and essentially all cells have receptors that respond to the presence of mature TGF-P in the stroma. In the periodontium, TGF-P stimulates fibroblast and osteoblast proliferation during connective tissue or bone remodeling (Sect. 10.1.3), and maintains the proliferation of dentally attached epithelial cells (Sect. 5.2.3). The linker domains that connect calcium binding domains in fibrillin are identical to the sequence of protein receptors that bind to TGF-P (Sect. 6.1.1). 

The effeets of 98a on murine bone eells in vitro and in ovarieetomized (OVx) miee showed that 98a at 10 M and 10 M inhibited osteoelastogenesis of bone marrow eells and displayed eoneentration dependenee 98a (10 M) stimulated osteoblast proliferation, differentiation and mineralization. 

Regulation of osteoblast proliferation by leukemia inhibitory factor. J. Bone Min. Res. 6 1277-1283. 

Cell attachment, proliferation, and differentiation over time on a material are indications of the cellular compatibility of the material and determine the suitability of the material for tissue engineering applications. Osteoblast proliferation on chitosan-genipin scaffolds was assessed using Almar Blue assay and foimd to be satisfactory considering that the number of cells attached to the genipin-crossUnked chitosan scaffolds after 1, 3, and 7 days of cell culmre increased with time [67]. 

Other studies looked into the use of composite microspheres of PLGA/HA in bone regeneration study results showed that the controlled release system of these microspheres functioned to improve osteoblast proliferation and also enabled upregulation of the key osteogenic enzyme alkaline phosphatase (ALP) [168]. 

The human venous plasma and whole blood contain amino acids and the successful identification and quantification of those amino have been previously reported [28, 29]. Conconi et al. [30] reported that the amino adds (lysine, threonine, methionine, tryptophan, arginine, which are all present in the DMEM solutions) increased both the osteoblast proliferation and alkaline phosphatase activity of rat osteoblasts cultured in vitro. Imamura et al. [31] and Tentorio and Canova [32] separately showed that the amino acid lysine adsorbs itself on pure metallic Ti and on amorphous Ti hydrous oxide surfaces, respectively, at neutral pH values. While the inoiganic SBF solutions cannot provide any practical means of producing synthetic biomaterials with some amino acids adsorbed on their surfaces, DMEM solutions can provide unique biomaterial surfaces already containing adsorbed amino acids. 

PU) composite Poly(lactic acid) (PLA)/CNT composite Increasing osteoblast proliferation. 

Cool SM, Kenny B, Wu A, Nurcombe V, Trau M, Cassady Al, et al. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) composite biomaterials for bone tissue regeneration in vitro performance assessed by osteoblast proliferation, osteoclast adhesion and resorption, and macrophage proinflammatory response. J Biomed Mater Res Part A 2007 599-610. 

The first reported use of CNTs as an osteogenic biomaterial was in 2002 by Supronowicz et al. [41]. To promote ceU growth and adhesion, MWCNTs were incorporated into a PLA to fabricate conductive PLA/MWCNT nanocomposites. Osteoblast cells were seeded onto the surface and then exposed to alternating current stimulation (10 micro A at 10 Hz). Their results showed an increase in osteoblast proliferation by 46% after 2 days and increased extracellular calcium by 307% after 21 consecutive days. 

Han P, et al. Improved osteoblast proliferation, differentiation and minerahzation on nanophase Ti6A14V. ChwMerf7 2011 124(2) 273-9. 

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