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Electrical lysis

Wang, H.-Y., Bhunia, A. K., and Lu, C. (2006). A microfluidic flow-through device for high throughput electrical lysis of bacterial cells based on continuous DC voltage. Biosens. Bioelectron. 22,582-588. [Pg.44]

Because removing a cell from its growth substrate alters many of the biological processes occurring in the cell, its detachment increases the difficulty in analyzing the cell and the variation from its normal function. Thus, in order to obtain the most accurate portrait of the cell s internal environment, it must be sampled quickly after removal from the substrate. Two innovative techniques, laser cavitation and fast electrical lysis, allow for rapid sampling of substrate-bound cells. [Pg.430]

To circumvent the expense and complication of using a pulsed laser, a fast electrical lysis procedure for single-cell analysis has also been developed (Figure 14.1c). - This setup takes advantage of gold conduetivity, with both the capillary and the coverslip on which cells are grown coated... [Pg.430]

FIGURE 14.1 Recently developed whole cell sampling schemes, (a) Continuous cell introduction. (From Chen, S. andLillard, S.J., AnaZ. Chem., 73, 111, 2001. With permission.) (b) Laser cavitation. (From Sims, C.E. et al.. Anal. Chem., 70, 4570, 1998. With permission.) (c) Fast electrical lysis. (From Han, R, et al.. Anal. Chem., 75, 3688, 2003. With permission.)... [Pg.431]

Han, F. et al.. Fast electrical lysis of cells for capillary electrophoresis. Anal. Chem., 75, 3688, 2003. [Pg.441]

In general, cell lysis is performed by either chemical or physical means to rupture the cell phospholipid membrane with the predominant goal of extracting various intracellular contents. On microfluidic devices, there are many cell lysis methods that have been developed, such as chemical lysis, mechanical lysis, electrical lysis, thermal lysis, and other lysis methods. Some of them have been integrated on chip for chemical cytometry, but others are rather difficult to be integrated on chip. [Pg.415]

Electrical lysis of cells on a microfludic platform has been an area of extensive research interest due to its speed and reagentless procedure compared to chemical and mechanical methods. [Pg.416]

The development of more rapid and less invasive lysis protocols is a cmcial area that needs to be improved. The extent to which the lysis process alters cellular behavior is still unclear. Particularly, the intracellular molecules such as mRNAs and regulatory proteins may respond to stress conditions rapidly. Faster and more controlled lysis methods will provide a window through which the natural state of dynamic intracellular environments can be studied. Important advances in several areas are expected, including laser-based lysis and strategies that carefully combine chemical and electrical lysis methods by utilizing microenvironmental controls within a device. [Pg.420]

When a large electric field is applied across a cell, the transmembrane potential is disrupted and pores are formed on the surface of the membrane. This phenomenon is called electroporation and is often used for gene transfection. As conventionally implemented, the process is reversible, and when the electric field is terminated, the pores close. The phenomenon can also be used to cause permanent disruption of the membrane, effectively lysing the cell. There have been several reports (Ml the use of electric lysis techniques in micM ofluidic devices [9-11]. Of particular interest, fast lysis of individual cells ( 33 ms) by electric pulses for chemical cytometry was demonstrated in a micM ofluidic platform [12]. These extremely rapid lysis methods which minimize unwanted effects of slow lysis (that may bias the results) make these techniques favorable for protein analysis when compared to chemical lysis techniques. One drawback of electric lysis is that much of the cell membrane, subcellular structures, and the nucleus may remain intact and thus can clog the channel or adhere to the surface, affecting the separation and limiting the capacity for reuse. [Pg.3021]

Major Applications Electrical lysis of cells, detecting anion transpost mechanism at the tono-plast,4 as tracers and for preparing conjugates of organic inorganic substancesi Safety/Toxicity No data available... [Pg.293]

Veltwisch and colleagues45 studied the reaction of OH with several sulfoxides by pulse radiolysis using electrical conductivity for the detection of formation or disappearance of ions. Pulse radio lysis of N20-saturated aqueous solution of DMSO (10-3m) leads to a decrease in conductivity at basic pH (pH = 9.0) and an increase in conductivity at acidic pH (pH = 4.4). This is explained by the reactions... [Pg.899]

The word electrolysis derives from the Greek words lysis, meaning splitting or cleavage, and the root electro, meaning charge or electricity. Strictly, then, electrolysis involves electrochemical bond cleavage. [Pg.283]

Loss of hemoglobin by lysed red blood cells (cell membrane lysis). Controlled hemolysis (induced osmotically or electrically) yields hemoglobin-free erythrocyte ghosts . [Pg.59]

In this chapter, we deal with two aspects of the connection between chemistry and electricity. The first is electrolysis, the splitting (lysis) of compounds when electricity passes through the solutions involved. The second topic is galvanic cell action, which is the generation of electricity (a flow of electrons) during chemical reaction. [Pg.327]

Cell lysis under a high electric field is referred to as electroporation [6], Under these conditions, the cell membrane experiences dramatic changes in permeability to macromolecules. The main applications of the electroporation include the electrotransformation of cells and the electroporative gene transfer by the uptake of foreign DNA or RNA (in plants, animals, bacteria, and yeast). The electric field generates permeable microspores at the cell membrane, so that the nucleic acid can be introduced by electroosmosis or diffusion. [Pg.342]

Cell lysis on a chip has been carried out by several different approaches. Detergents have been used to lyse cells on a chip. Thermal lysing on a chip has been carried out by placing the cell in the sample reservoir and then raising the temperature of the chip [50], A practical approach for microchip applications is lysis by electroporation. Since fluids are moved around on chips by the application of an electric field, its use in cell lysis is an obvious choice. [Pg.372]

Electrolysis — (Greek . ..lysis - splitting) Decomposition of a material by application of an electrical voltage resulting in a flow of electric current associated with electroreduction at a cathode and electrooxidation at an anode. [Pg.222]

Generally, capillaries with inner diameters of 5-25 pm are used to inject and analyze single cells in CE. With such small capillaries, several features can be achieved, including low risk of injection of more than one cell at a time, low dilution factors after cell lysis, high resolving power, and rapidity at high electric field strengths. It is important to keep a very small dilution factor, as the... [Pg.897]

The transfer of intracellular K" into ECF invariably occurs in acidosis as H shifts intraceHularly and shifts outward to maintain electrical neutrality. As a general rule, K concentrations are expected to rise 0.2 to 0.7 mmol/L for every 0.1 unit drop in pH. When the underlying cause of the acidosis is treated, normokalemia will rapidly be restored. Extracellular redistribution of may also occur in (1) dehydration, (2) shock with tissue hypoxia, (3) insulin deficiency (e.g., diabetic ketoacidosis), (4) massive intravascular or extracorporeal hemolysis, (5) severe burns, (6) tumor lysis syndrome, and (7) violent muscular activity, such as that occurring in status epilepticus. Finally, important iatrogenic causes of redistribution hyperkalemia include digoxin toxicity and P adrenergic blockade, especially in patients with diabetes or on dialysis. ... [Pg.1756]

Conclusion. We have expressed eukaryotic CK in . coli and have demonstrated that active protein can be recovered from the resulting inclusion bodies. Based on the parameters tested, this protein appears to be very similar to CK purified from electric organ tissue. The recovery scheme is simple, involving cell lysis and overnight extraction with a detergent-containing buffer followed by sonication and isolation of... [Pg.166]


See other pages where Electrical lysis is mentioned: [Pg.942]    [Pg.179]    [Pg.430]    [Pg.416]    [Pg.2483]    [Pg.3021]    [Pg.293]    [Pg.1514]    [Pg.1854]    [Pg.306]    [Pg.306]    [Pg.942]    [Pg.179]    [Pg.430]    [Pg.416]    [Pg.2483]    [Pg.3021]    [Pg.293]    [Pg.1514]    [Pg.1854]    [Pg.306]    [Pg.306]    [Pg.514]    [Pg.564]    [Pg.4]    [Pg.138]    [Pg.492]    [Pg.290]    [Pg.342]    [Pg.265]    [Pg.217]    [Pg.5]    [Pg.316]    [Pg.748]    [Pg.101]    [Pg.697]    [Pg.349]    [Pg.363]    [Pg.523]    [Pg.137]   
See also in sourсe #XX -- [ Pg.430 ]




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