Intracellular contents

Although not as common as hypercalcemia, tumor lysis syndrome may cause significant morbidity and mortality if adequate prophylaxis and treatment are not instituted. Tumor lysis syndrome is the result of rapid destruction of malignant cells with subsequent release of intracellular contents into the circulation. 

Jara, J. R., Martinez-Liarte, J. H., Solano, F., and Penafiel, R. (1988). Transport of L-tyrosine by B16 F10 melanoma cells the effect of the intracellular content of other amino acids. J. Cell Sci. 97 479-485. 

Several mechanisms are involved in the permeability through Caco-2 cells. In order to obtain a more pure measure of membrane permeability, an experimental method based on ghost erythrocytes (red blood cells which have been emptied of their intracellular content) and diffusion constant measurements using nuclear magnetic resonance (NMR) has been proposed [108]. 

Cb+ compound concentration of added compound (mM) intracellular content of Cr (mg of Cr/g of dry weight)... 

ELISA has been used for measuring caspase activity. For the ELISA of intracellular caspase activity at the very early stages of apoptosis, apoptotic cells are first lysed to isolate their intracellular contents. Different caspase activities in the cell lysate can then be determined by the addition of a caspase-specific tetrapeptide substrate that is conjugated to the color reporter molecule p-nitroanilide (pNA) (e.g., DEVD-pNA for caspase-3 and lETD-pNA for caspase-8). The cleavage of the substrate peptide by the caspase releases the chromophore pNA, which can be... 

Separation from culture media or broth is the primary step in collecting the product found either in cells (sohd) or medium (liquid). This initial separation step is engineered based on cell size and density differences between solid and liquid (Table 4.10). In the case where the recombinant product is localized in the intracellular content such as the cytoplasm or inclusion bodies, which are highly insoluble particles found in bacteria, the cells are hrst isolated from the medium and then disrupted to collect the recombinant protein fraction. A number of cell disruption techniques have been developed to facilitate this step, and some are listed in Table 4.11. 

Nebert, D.W. and Bausserman, L.L. (1970). Fate of inducer during induction of aryl hydrocarbon hydroxylase activity in mammalian cell culture. II. Levels of intracellular content of polycyclic hydrocarbon during enzyme induction and decay ) Mol. Pharmacol 6,304. 

A cell is enclosed by a lipid bilayer known as the plasma membrane. In Vignette 1.2 in Chapter 1 we discussed an example of a membrane, a complex structure with a mosaic of embedded or adsorbed moieties such as proteins. It is these membranes that protect the intracellular contents from the exterior environment of the cells and regulate the transport of materials into and out of the cells. They can also act as signal transducers and control the electrical excitation in the nervous system by altering the (membrane) permeability to particular ions in response to stimuli. Such electrical activities can propagate over long distances and represent one of the most spectacular of the membrane functions. 

However, in spite of the significant diversity in the quantitative evaluation of platelet adhesion, the platelets adhered onto heparinized surfaces are neither aggregated nor activated 4-78>. In other words, the intracellular contents of the adhered platelets, which may otherwise contribute to the blood clotting process, is not evolved into the bloodstream. This conclusion is verified by the results of Table 12, which indicate that the increased platelet adhesion does not result in a worse thrombo-resistance the blood clotting time at a HCP surface is an order of magnitude higher than that at the surface of the initial non-heparinized polymer. 

The plasma membrane is a delicate, semipermeable, sheetlike covering for the entire cell. Forming an enclosure prevents gross loss of the intracellular contents the semipermeable character of the membrane permits the selective absorption of nutrients and the selective removal of metabolic waste products. In many plant and bacterial (but not animal) cells, a cell wall encompasses the plasma membrane. The cell wall is a more porous structure than the plasma membrane, but it is mechanically stronger because it is constructed of a covalently cross-linked, three-dimensional network. The cell wall maintains a cell s three-dimensional form when it is under stress. 

Like spinal cord trauma, traumatic head injury consists of a primary injury, attributable to the mechanical insult itself, and a secondary injury, attributable to the series of systemic and local neurochemical changes that occur in brain after the initial traumatic insult (Klussmann and Martin-Villalba, 2005). The primary injury causes a rapid deformation of brain tissues, leading to rupture of neural cell membranes, release of intracellular contents, and disruption of blood flow and breakdown of the blood-brain barrier. In contrast, secondary injury to the brain tissue includes many neurochemical alterations such as release of cytokines, glial cell reactions involving both activated microglia and astroglia, and demyelination... 

The detergent lysis is relatively fast, and the release of intracellular contents causes dramatic changes in the physical properties, such as viscosity and solution consistency. For example, Levy et al. [5] carried out the lysis of a suspension of E. coli C600 with 0.2 M NaOH containing 1% w/v of SDS. The cell suspension and the detergent solution were mixed in a 1 1 volumetric ratio. The reaction was carried out in the narrow gap of a coaxial viscometer. The viscosity increased rapidly with addition of the SDS-NaOH solution to the cell suspension. The maximum viscosity occurred in 100 seconds, suggesting that all the cells had been lysed in that time. 

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