Colon cancer lines

Johnson MT, Rreft E, N Da DD, Neuse EW, van Rensburg CEJ (2003) The cytotoxic activity of macromolecular ferrocene conjugates against the Colo 320 DM human colon cancer line. J Inorg Organomet Polym 13 255-267... 

The mistletoe lectins inhibited the growth of multidrug-resistant human colon cancer line HT 29 (mdr) in vitro Numerous studies have reported immunostimulatoiy and proapoptotic effects for mistletoe extracts, preparations, and components. The mistletoe extracts and preparations also significantly inhibited carcinogenesis and metastatis. Animal studies have shown that mistletoe preparations suppressed side effects when used as an adjunct to chemotherapy and radiation therapy. ... 

The in vivo anti-tumor activity was evaluated with a human colonic cancer line, HT-29, whieh was inoculated into the abdominal skin of nude mice. 5.6mg/kg KRN5500 could be administered iv only once, because this dose of KRN5500 always induced irreversible inflammatory change in the tail of mice. We therefore evaluated the difference in anti-tumor activity between KRN5500 and KRN/m after a single injection of the two drags. 

AVIVI-GREEN c, PLOAK-CHARCON s, MADAR z, SCHWARTZ B (2002) Different molecule events account for butyrate-induced apoptosis in tiw human colon cancer cell line. J. Nutr. 132 1812-18. 

GAMET L, DAViAUD D, DENis-POUXViEL 0, REMESY 0, MURAT J 0 (1992) Effects of shoit-chain fatty acids on growth and differentiation of the human colon-cancer cell line HT29. Int J Cancer. 52 286-9. 

Oxaliplatin (Eloxatin ) is similar to other platinum analogs (e.g., cisplatin) in that it binds to the N-7 position of guanine, which results in cross-linking of DNA and double-stranded DNA breaks.26,40 Oxaliplatin differs from cisplatin in that the DNA damage induced by oxaliplatin may not be as easily recognized by DNA repair genes often seen in colorectal cancer. Oxaliplatin, in combination with 5-FU-based regimens, is indicated for the first- and second-line treatment of metastatic colon cancer, as well as the adjuvant treatment of colon cancer. 

Colon cancer A disease in which cells in the lining of the colon become malignant and proliferate without control. Often referred to as colorectal cancer to include cells of the rectum. 

Another human colonic cancer cell line is T84 this develops monolayers of high TER ( 1000 Q cm2) when grown on permeable supports, but cells are not well differentiated and have been described as resembling a colonic crypt cell phenotype. Hence, these cells have been used mainly in studies of epithelial ion transport and are generally not considered to be adequate for drug transport studies, particularly with respect to carrier-mediated processes [10, 79, 82-84]. 

Bonnesen C, Eggleston I M and Hayes J D (2001), Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines , Cancer Rese, 61, 6120-6130. 

BS, Bacillus subtilis CA, Candida albicans SA, Staphylococcus aureus SC, Saccharomyces cerevisiae TM, Trichophyton mentagrophytes PN, Penicillium nonatum KB, LOVO (colon cancer), P388 are cell lines in cytotoxic activity testings. 

Experiments conducted in the early 1980s showed that lymphocytes incubated in vitro with IL-2 could subsequently kill a range of cultured cancer cell lines, including melanoma and colon cancer cells. These latter cancers do not respond well to conventional therapies. Subsequent investigations showed that cancer cell destruction was mediated by IL-2-stimulated NK cells (i.e. LAK cells). Similar responses were seen in animal models upon administration of LAK cells activated in vitro using IL-2. 

A. I. Haza, B. Glinghammar, A. Grandien and J. Rafter, Effect of colonic luminal components on induction of apoptosis in human colonic cell lines, Nutr. Cancer, 2000, 36(1), 79. 

K. Schlottman, F. P. Wachs, R. C. Krieg, F. Kullmann, J. Scholmerich and G. Rogler, Characterization of bile salt-induced apoptosis in colon cancer cell lines. Cancer Res., 2000, 60(15), 4270. 

S. Lechner, U. Muller-Ladner, K. Schlottmann, B. Jung, M. McClelland, J. Ruschoff, J. Welsh, J. Scholmerich and F. Kullmann, Bile acids mimic oxidative stress induced up-regulation of thioredoxin reductase in colon cancer cell lines, Carcinogenesis, 2002, 23(8), 1281. 

This HDAC inhibitor (Fig. 15) was isolated from a cyanobacterium Symplaca sp. collected from the Florida Keys. This depsipeptide was described by Leusch and coworkers in lOOSP It has been shown to be potent at low nanomolar ranges against mammary cancer cell lines MDA-MB-231, HT29 colon cancer cells, IMR-32 neuroblastoma cells and U20S fibroblastic osteosarcoma cells. ... 

Nakano K, Mizuno T, Sowa Y, Orita T, Yoshino T, Okuyama Y, Fujita T, Ohtani-Fujita N, Matsukawa Y, Tokino T, Yamagishi H, Oka T, Nomura H, Sakai T. (1997) Butyrate activates the WAFl/Cipl gene promoter through Spl sites in a p53-negative human colon cancer cell line. J Biol Chem 212 22199-22206. 

Moved] Cranberry fruit of Early Black cultivar was fractionated chromatographically and fractions were analyzed for flavonoid content. The effects of the flavonoid fractions and ursolic acid, an abundant triterpenoid in cranberry peel, were assessed in two models of colon cancer and one model of breast cancer. Clonogenic soft agar assays were used to determine the effect of these compounds on tumor colony formation in HCT-116, HT-29 and MCF-7 cells. MTT and trypan blue assays were performed to assess their ability to inhibit tumor cell proliferation. TUNEL assays were performed to assess apop-totic response to the cranberry compounds. The proanthocyanidins inhibited tumor colony formation in HCT-116 and HT-29 cells in a dose-dependent manner, with greater effect on the HCT-116 cell line. Ursolic acid strongly inhibited tumor colony formation in both colon cell lines. These compounds also decreased proliferation in all three tumor cell lines with the HCT-116 cell line most strongly affected. (150 words)... 

The cancer cell lines SW480 and SW620 are two colon cancer cell lines that were obtained from the same patient the SW480 cell line was obtained from the primary tumor, and the SW620 cell line was obtained from a metastatic site. (Adapted from Seeram et al., 2004)... 

Roche has reported a 2,4-diaminopyrimidine 42 (Ro-4584820) to be a Phase I clinical candidate [48,103]. Compound 42 is a pan-CDK inhibitor (CDKl K = 0.001 ptM CDK2 K[ = 0.003 p.M CDK4 K[ = 0.001 p.M), consequently it induces both G1 and G2 cell cycle arrest in tumor cell lines. It is a potent inhibitor of tumor cell proliferation in both the HCT-116 colon cancer (IC50 = 0.080 tiM) and the H460A (IC50 = 0.055 p.M) lung cancer cell lines. 

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