Chip device

STATE-OF-THE-ART OF BIOOBJECTS ASSAY IN MICROFLUIDIC LAB-ON-A-CHIP DEVICES... 

The interdigital feed can be fed in a counter-flow or co-flow orientation the first principle is realized in metal/stainless steel or silicon/stainless steel devices [39, 41], the latter in glass chip devices [40, 44 6]. 

Fig. 2.6.11 Flow dispersion profiles obtained with (a) a capillary, (b) with a model microfluidic chip device containing a channel enlargement, directly connected to a capillary and (c) with the same microfluidic chip connected to a capillary via a small mixing volume. A sketch of the model microfluidic device is placed at the right side of each image, drawn to...

Mapping of transport parameters in complex pore spaces is of interest for many respects. Apart from classical porous materials such as rock, brick, paper and tissue, one can think of objects used in microsystem technology. Recent developments such as lab-on-a-chip devices require detailed knowledge of transport properties. More detailed information can be found in new journals such as Lab on a Chip [1] and Microfluidics and Nanofluidics [2], for example, devoted especially to this subject. Electrokinetic effects in microscopic pore spaces are discussed in Ref. [3]. 

The application of polymer monoliths in 2D separations, however, is very attractive in that polymer-based packing materials can provide a high performance, chemically stable stationary phase, and better recovery of biological molecules, namely proteins and peptides, even in comparison with C18 phases on silica particles with wide mesopores (Tanaka et al., 1990). Microchip fabrication for 2D HPLC has been disclosed in a recent patent, based on polymer monoliths (Corso et al., 2003). This separation system consists of stacked separation blocks, namely, the first block for ion exchange (strong cation exchange) and the second block for reversed-phase separation. This layered separation chip device also contains an electrospray interface microfabricated on chip (a polymer monolith/... 

Recent developments in microsystems technology have led to the widespread application of microfabrication techniques for the production of sensor platforms. These techniques have had a major impact on the development of so-called Lab-on-a-Chip devices. The major application areas for theses devices are biomedical diagnostics, industrial process monitoring, environmental monitoring, drug discovery, and defence. In the context of biomedical diagnostic applications, for example, such devices are intended to provide quantitative chemical or biochemical information on samples such as blood, sweat and saliva while using minimal sample volume. 

M.J. Heller, A.H. Forster, and E. Tu, Active microelectronic chip devices which utilize controlled electrophoretic fields for multiplex DNA hybridization and other genomic applications. Electrophoresis 21, 157-164 (2000). 

Lee, K. J. Tosser, K. A. Nuzzo, R. G. 2005. Fabrication of stable metallic patterns embedded in poly(dimethylsiloxane) and model applications in non-planar electronic and lab-on-a-chip device patterning. Adv. Funct. Mater. 15 557-566. 

In order to achieve improved nanofabrication performance, novel functional block copolymer systems are strongly desired. Many researchers have recognized this, and novel functional systems such as metal-containing block copolymer systems have significantly simplified and improved nanofabrication processes. The combination of top-down microscale patterns with the bottom-up nanopatterns are attractive for integrating functional nanostructures into multipurpose on-chip devices. However, in order to use these materials in real-time applications, further development is still needed. More ground-shaking discoveries are needed and are also fully expected. 

The limit for the operating temperature of CMOS-microhotplates can be extended by using the microhotplate that was presented in Sect. 4.3. We now detail high-temperature microhotplates with Pt-resistors that have been realized as a single-chip device with integrated circuitry. While the aluminum-based devices presented in Sect. 4.1 were limited to 350 °C, these improved microhotplates can be heated to temperatures up to 500 °C. As the typical resistance value of the Pt-resistor is between 50 and 100 Q, a chip architecture adapted to the low temperature sensor resistance was developed. The system performance was assessed, and chemical measurements have been performed that demonstrate the full functionality of the chip. 

The analysis of biomolecules by AFM is sometimes [3] referred to as surface biology, as opposed to the so-called test-tube biology, because the immobilisation of oligonucleotides on sohd surfaces is central to the design, fabrication and operation of DNA-based microdevices, such as biosensors, DNA micro- and nanoarrays, microPCR and lab-on-a-chip devices. As the analysed biomolecules are in close contact and very often in intimate interaction with the surface, sample preparation for the AFM analysis of surface-immobihsed biomolecules is both critical and dehcate. The biomolecules need to be firmly anchored on the substrate, which has to have a sufficiently minimal or easily discriminated topography [1]. The Kleinschmidt method [6] for the DNA... 

The versatility of lab-on-a-chip devices has been exploited for developing a novel and effective protocol for rapid screening/waming followed by detailed... 

Unlike capillary electrophoresis, wherein absorbance detection is probably the most commonly utilized technique, absorbance detection on lab-on-a-chip devices has seen only a handful of applications. This can be attributed to the extremely small microchannel depths evident on microchip devices, which are typically on the order of 10 pm. These extremely small channel depths result in absorbance pathlengths that seriously limit the sensitivity of absorbance-based techniques. The Collins group has shown, however, that by capitalizing on low conductivity non-aqueous buffer systems, microchannel depths can be increased to as much as 100 pm without seeing detrimental Joule heating effects that would otherwise compromise separation efficiencies in such a large cross-sectional microchannel [38],... 

Efforts toward integrating SPE onto a lab-on-a-chip device are currently being investigated by the Collins group. Two complementary approaches are being pursued. One approach is to use small-diameter, Cl8 functionalized silica beads that are packed into a microchannel to form an extraction bed [46], A sample solution containing trace levels of explosives is electrokinetically directed across the microcolumn bed, causing the hydrophobic explosive molecules to adsorb onto the stationary phase with nearly 100% efficiency. Subsequently,... 

Microfluidic chip devices are also shown to be attractive platforms for performing microscale voltammetric analysis and for integrating voltammetric procedures (linear-sweep, square-wave and adsorptive-stripping voltammetry) with on-chip chemical reactions and fluid manipulations [97]. 

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