Flow Cytometer Lab-on-a-Chip Devices

Flow Cytometer Lab-on-a-Chip Devices, Fig. 4 Integrated optical craiponents on a PDMS-based flow cytometer (a) design of PDMS observation channel with embedded optical fibers (arrangcanent shown with 45° angle between excitation and detection optical fibers). 

Flow Cytometer Lab-on-a-Chip Devices, Fig. 5 Microfabricated Coulter counter (a) scanning electron microscope image of the Coulter counter. The 3.5 pm-deep reservoirs and the inner Ti/Pt electrodes, which control the voltage applied to the pore but pass no current, are only partially shown. The inset shows a magnified view of this device s pore, which has dimensions 5.1 X 1.5 X 1.0 pm, and (b) relative changes in baseline current versus time for a monodisperse solution of 87 nm diameter latex colloids and a polydisperse solution of latex colloids with diameters 460, 500, 560, and 640 nm [5]... 

Flow Cytometer Lab-on-a-Chip Devices, Fig. 6 Micromachined impedance flow cytometer (a) side schematic view of the microchannel showing a particle passing over three electrodes (A, B, and C). The impedance signal is measured differently (Zac - Zbc). As the distance between two measurement areas and time separating the signal spikes are known, the speed of the particle can be calculated, and (b) signal in-phase amplitude of 2,000 erythrocytes and ghost cells recorded simultaneously for two frequencies [9]... 

Flow Cytometer Lab-on-a-Chip Devices, Fig. 7 (continued) 488 ran laser are focused through the same lens onto the microfluidic chip. The presence of a cell in the analysis region is detected by a photodiode and the fluorescence of that cell is measured by the PMT. Based on a gating of the fluorescent signal, the AOM is triggered to optically switch the cell (GFP-positive cells are switched... 

Flow Cytometer Lab-on-a-Chip Devices, Fig. 8 Flow cytometer Lab-on-a-Chip (a) setup and SEM image of the second-generation micro cell sorter chip with integrated holding/culturing chamber (a sheathing buffer inlet,... 

Flow Cytometer Lab-on-a-Chip Devices, Fig. 8 (continued) during cultivation g, waste outlet) [14], (b) integrated impedance flow cytometer (A cell dipping electrodes, B detection electrodes, C sorting/trapping... 

We can envision that the future flow cytometer Lab-on-a-Chip devices will miniaturize and integrate monolithically a variety of optical components like light sources, photodetectors, filters, waveguides and lenses that can detect multiple fluorescence col-... 

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