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Cellular analysis

Single isolated neurons were investigated with respect to differential peptide profiling [45, 46]. The spreading of individual neuronal cells onto a MALDI target by mechanical elongation allows the differentiation of subcellular areas, even with an instrument that has a lateral resolution (a laser focus diameter) of ca. 100 pm. Mechanical stretching provides an efficient approach to circumvent instrumental [Pg.156]


Carlsson A (1993) Thirty years of dopamine research. Adv Neurol 60 1-610 Castellucci VF, Carew TJ, Kandel ER (1978) Cellular analysis of long-term habituation of the gill-withdrawal reflex of Aplysia californica. Science 202(4374) 1306-8 Cepeda C, Buchwald NA, Levine MS (1993) Neuromodulatory actions of dopamine in the neostriatum are dependent upon the excitatory amino acid receptor subtypes activated. Proc Natl Acad Sci USA 90(20) 9576-80... [Pg.468]

Cell retention, manipulation, and subsequent cellular analysis can be all achieved on-chip. Cell retention can be achieved by using slit- or weir-type filters, or by cell adhesion. In addition, fluid flow optical trapping and dielectrophoresis (DEP) have been exploited to manipulate and retain cells. [Pg.251]

Sometimes, cellular analysis was performed with cells in a flow stream, which is also termed flow cytometry or FACS in the field of cell biology. For instance, human blood cell (WBC, RBC) rheology was studied in channels fabricated on the Si-Pyrex substrates. The channels were either uncoated or coated with albumin [825]. [Pg.280]

Tokuyama, T., Fujii, S.I., Abo, M., Okubo, A., Cellular analysis systems of histamine release on a microchip. Micro Total Analysis Systems Proceedings pTAS 2002 symposium, 6th, Nara, Japan, Nov.3-7, 2002, 832-834. [Pg.468]

The book is divided into several chapters which include micromachining methods, microfluidic operations (microfluidic flow, sample introduction, sample preconcentration), chemical separations, detection technology, and various chemical and biochemical analysis (applications on cellular analysis, nucleic acid analysis, and protein analysis). Emphasis will be placed on analytical applications although the basic principles about micromachining and fluid flow and control will also be covered only to the extent that their understanding will assist the exploitation of the microfluidic technology on analytical applications. [Pg.515]

Cellular analysis will depend on cell source, with higher risk cell sources requiring more extensive in vitro, in vivo, and ex vivo analysis. Genetic stability and cell phenotypic analysis are also focus areas in preclinical studies, since they provide evidence of appropriate regeneration and function of the cellular component in the neo-organ product. [Pg.819]

Thrall, R.S., Barton, R.W., D Amato, D.A. and Sulavik, S.B. (1982). Differential cellular analysis of bronchoalveolar lavage fluid obtained at various st es during the development of bleomycin induced pulmonary fibrosis in the rat. Am. Rev. Respir. Dis. 126, 488-492. [Pg.225]

A recognized flow cytometry procedure for standard indirect antibody staining for cellular analysis and cell sorting is discussed. A protocol for cell surface or intracellular antibody staining of viable cells is... [Pg.331]

Vidal, M., Goudreau, N., Cornille, E, Cussac, D., Gincel, E., and Garbay, C. (1999). Molecular and cellular analysis of Grb2 SH3 domain mutants Interaction with Sos and dynamin.y. Mol. Biol. 290, 717-730. [Pg.260]

Microscale blood vessels can also be modeled by microfluidic channels [8]. This work may be applicable to studies of blood vessels and endo-thelia. As in the microchamber cell culture method, detection devices such as microelectrodes can be fabricated in microchannels for on-chip cellular analysis. [Pg.329]

Droplet-based Lab-on-a-Chip devices represent a class of microfluidic systems that have been developed to miniaturize chemical, biochemical, or cellular analysis. These devices utilize various droplet dispensing and manipulatiOTi mechanisms to isolate and control liquid-phase droplets of samples and/or reagents surrounded by a gas or an immiscible liquid. The droplets may be completely surrounded by the medium or may be placed onto a solid surface whose material properties are chosen to provide droplet confinement, chemical functionalization, or control of droplet motion. The devices also have some type of assay readout method with associated hardware, software, and data display. [Pg.635]

In cellular applications, single-cell analysis is preferred to the traditional bulk cellular analysis because the former method can study cellular heterogeneity and provide information on... [Pg.2006]

Castellucci, V.F., Carew, T.J. and Kandel, E.R. 1978. Cellular analysis of longterm habituation of the gill-withdrawal reflex of Aplysia Califomica. Science, 202,1306-8. [Pg.38]

Lockyer, N. P., Vickerman J. C. (2004) Progress in cellular analysis using ToF-SIMS. ApplSurfSci, 231-232, 377-384. [Pg.264]

J.J. Inglis, et al. Collagen-induced arthritis as a model of hyperalgesia Functional and cellular analysis of the analgesic actions of tumor necrosis factor blockade. Arthritis Rheum., 56 (12), 4015-4023, 2007. http //dx.doi.org/10.1002/art.23063. [Pg.407]

CANARY Cellular Analysis and Notification of Antigen Risks and Yields... [Pg.307]


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See also in sourсe #XX -- [ Pg.156 , Pg.157 ]




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