Cell sorting

Measurements of particular properties of a compound or substance (enzyme kinetics, reaction kinetics, FACS, fluorescence-activat cell sorting, assay). 

J. Porter, C. Edwards, J. A. W. Morgan, and R. W. Pickup, Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting, Appl. Environ. Microbiol. 59 3327 (1993). 

Perfetto, S. P. Ambrozak, D. R. Koup, R. A. Roederer, M. Measuring containment of viable infectious cell sorting in high-velocity cell sorters. Cytometry (Pt. A) 2003, 52A, 122-130. 

Darvey, H. M. Kell, D. B. Flow cytometry and cell sorting of heterogenous microbial populations the importance of single cell analysis. Microbiol. Rev. 1997, 60, 641-696. 

The ability to display proteins on the surface of an organism has been exploited as a screening method, typically in conjunction with fluorescence-activated cell sorting (FACS) [37,47,48]... 

Becker, S., Schmoldt, H.U., Adams, T.M. et al. (2004) Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. Current Opinion in Biotechnology, 15, 323-329. 

Ibrahim, S.F. and van den Engh, G. (2007) Flow cytometry and cell sorting. Advances in Biochemical Engineering/Biotechnology, 106, 19-39. 

The labeled glycans may be analyzed by cell sorting after staining with fluorescently modified streptavidin. Alternatively, the cells may be lysed and the labeled glycans isolated using immobilized streptavidin. 

The chemical composition of particles can be just as varied as their shape. Commercial particles can consist of polymers or copolymers, inorganic constructs, metals and semiconductors, superparamagnetic composites, biodegradable constructs, and synthetic dendrimers and dendrons. Often, both the composition of a particle and its shape govern its suitability for a particular purpose. For instance, composite particles containing superparamagnetic iron oxide typically are used for small-scale affinity separations, especially for cell separations followed by flow cytometry analysis or fluorescence-activated cell sorting (FACS). Core-shell semiconductor particles, by... 

Abou-Samra, A.B., Freeman, M., Juppner, H., Uneno, S., and Segre, G.V. (1990) Characterization of fully active biotinylated parathyroid hormone analogs. Applications to fluorescence activated cell sorting of parathyroid hormone receptor bearing cells./. Biol. Chem. 265, 58-62. 

J. Kruger, K. Singh, A. O Neill, C. Jackson, A. Morrison, and P. O Brien, Development of a microfluidic device for fluorescence activated cell sorting. J. Micromech. Microeng. 12, 486-494 (2002). 

Cell potential, 9 607-609 standard, 75 750 Cell Saver, 3 719 Cells, encapsulation of, 76 454 Cell sorting, microfluidics in, 26 971-972 Cell-specific dendritic carriers, 26 797 Cell targeting, dendrimers in, 26 797-798 Cell thermo stating, 73 426 Cellular components inducers, herbicide damage to, 73 297-298 Cellular damage, in aging, 2 810 Cellular diseases, yeast as a model for, 26 496-497... 

Magnetic cell sorting, 26 971-972 Magnetic concentration and purification, 15 442-457. See also Magnetic separators... 

Magnetic (iron oxide core) microspheres have been imprinted with S-pro-pranolol. The magnetism allowed the facile separation of the imprinted beads from the liquid matrix. The particles exhibited the expected affinity towards the template molecule. This technique was also proposed as a putative tool for cell sorting [81]. 

---