Aplysia californica

Giant neuron of Aplysia californica Nj closed-end all-glass microelectrode Na- 40.3 mM 146)... 

Fig. 2. Arachidonic acid metabolites identified in Aplysia californica nervous tissue...

Stuart JN, Zhang X, Jakubowski JA, Romanova EV, Sweedler JV. 2003. Serotonin catabolism depends upon location of release characterization of sulfated and gamma-glutamy-lated serotonin metabolites in Aplysia californica. J Neuro-chem 84 1358. 

Arthurdendyus triangulatus NPF Moniezia expansa NPF Schistosoma japonicum NPF Schistosoma mansoni NPF Aplysia californica NPF Lymnaea stagnalis NPF Drosophila melanogaster NPF Lamprey NPY Homo sapiens NPY... 

Figure 7.3.1.5 300 MHz 1 H spectrum of Aplysia californica a-bag cell peptide [1-7]. The 5-nl detection cell contains 3.0 pg (3.3 nmol) of peptide. The LOD is 112ng (120pmol) for an acquisition time of 11.8 min. Adapted with permission from Olson, D. L., Peck, T. L., Webb, A. G., Magin, R. L. and Sweedler, J. V., Science, 1995, 270, 1967. Copyright (1995) American Association for the Advancement of Science...

Carlsson A (1993) Thirty years of dopamine research. Adv Neurol 60 1-610 Castellucci VF, Carew TJ, Kandel ER (1978) Cellular analysis of long-term habituation of the gill-withdrawal reflex of Aplysia californica. Science 202(4374) 1306-8 Cepeda C, Buchwald NA, Levine MS (1993) Neuromodulatory actions of dopamine in the neostriatum are dependent upon the excitatory amino acid receptor subtypes activated. Proc Natl Acad Sci USA 90(20) 9576-80... 

Nolen, T.G., Johnson, P.M., Kicklighter, C.K., and Capo, T., Ink secretion by the marine snail Aplysia californica enhances its ability to escape from a natural predator, J. Comp. Physiol., A176, 239, 1995. 

Baibas D, Zappulla JP, Angers S, Bouvier M, Castellucci VF, DesGroseillers L. Functional characterization of a novel serotonin receptor (5-HTap2) expressed in the CNS of Aplysia californica. J Neurochem 2002 80 335-345. 

Aplysia californica A. brasiliana A. fasciata A. vaccaria A. depilans... 

Interest continues in the isolation and structural elucidation of halogenated sesquiterpenoids produced by marine organisms (e.g. algae). Many of these compounds are chamigrane derivatives and new members of this group include prepacifenol epoxide (89)46 from Aplysia californica, elatol (90)47 from Laurencia elata, nidifidienol (91)48 from Laurencia nidifica, and the compounds (92) and (93)49 from... 

Halpem et al. [95] have reported their studies of neurons from the marine mollusc, Aplysia californica. In this study, the electrode of choice was a 30-pm diameter diamond microdisk electrode to study feeding patterns in the animal model based on extracellular measurements of 5-hydroxytyptamine. Apart from stable oxidation currents for the electrically-evoked release of serotonin from metacerebral cells, the key finding from this work was that the diamond electrode could be employed in both stimulation and recording of neurotransmitter release. 

A new halogenated monoterpenoid from Aplysia californica is (64) which is related to the previously reported (65) (Vol. 6, p. 20), although the latter probably has a different stereochemistry the previously reported (Vol. 6, p. 20) dibromotrichloro-monoterpenoid can now be assigned the structure (61 X = CH2Br) and a third new compound may tentatively be assigned the structure (66). ... 

Nucleoside Cyclic Phosphates. - Two novel fluorescent analogues of the secondary messenger molecule cyclic ADP-ribose (cADPR) have been pre-pared. Reaction of e-NAD (73) with sodium bromide in DMF in the presence of triethylamine gave the 9-cyclic ADP-ribose 74 whilst the enzyme catalysed cyclisation using Aplysia californica gives the N1-alkylated (equivalent to N7 in adenine) product. The novel cyclic etheno-CDP-ribose 75 was obtained by the enzymatic route and by the chemical cyclisation, albeit in poor yield in the latter case. A P NMR characterisation of cyclic ADP-ribose (cADPR) and its 2 -phospho-cyclic ADP-ribose has been published. ... 

Glycolipids moving along an identified axon of the marine mollusc, Aplysia californica, were synthesized and incorporated into intracytoplasmic membranes solely in the perikarion. Direct injection of tritiated sugar into the axon revealed no localized synthesis or exchange. There was no indication of any transfer into the axoplasm from the glia. The insertions of glycolipids into nascent membranes occurs co-ordinately with the insertion of protein components in the cell body. 

Gorbushin AM, Panchin YV, Iakovleva NV. In search of the origin of FREPs characterization of Aplysia californica fibrinogen-related proteins. Dev Comp Immunol. 2010 34 465-73. 

Neuropeptide. Isol. from a neuron of Aplysia californica. Vilim, F.S. et al. Peptides N.Y.), 1994, 15, 959 isol, hplc, ms)... 

SPECTRAL data PMR, Mass Spec organism Aplysia californica (Mollusca) reference 141... 

Early studies by Meulemans et al. used differential pulse voltammetry (see Chapter 11) to measure the concentration of electroactive drugs in the cytoplasm of cholinergic neurons of Aplysia californica and to determine rates of cellular uptake and clearance (108). Another report by Meulemans et al. described a needle tipped glass insulated platinum electrode for detection of intracellular serotonin in metacerebral neurons from Aplysia (109). Again differential pulse voltammetry was used to measure intracellular concentration of serotonin after cell stimulation, after injection of serotonin into the cell, and after extracellular treatment with tryptophan, a serotonin precursor. Serotonin concentration in resting untreated cells was determined to be 0.94 0.61 mM. Chen et al. have also used platinum electrodes with multiple pulse voltammetry to measure dopamine levels in the giant dopamine neuron of Planorbis comeus (110). 

This paper reports the values of KT, Na and Cl activities measured with ion-selective microelectrodes in certain identifiable neurons in the abdominal ganglion of Aplysia californica (nomenclature according to Frazier, et al., 1967). These measurements allow calculation of the equilibria potentials of the three ions thought to be most important in regulating intracellular voltage. The ion-selective microelectrode technique makes such measurements possible in small cells moreover these values can be compared amongst neurons which are anatomically and functionally distinct. 

Coggeshall, R.E. 1967. A light and electron microscope study of the abdominal ganglion of Aplysia californica. J. Neurophysiol. 

Frazier, W.T., E.R. Kandel, I. Kupferman, R. Waziri, and R.E. Coggeshall. 1967, Morphological and functional properties of identified neurons in the abdominal ganglion of Aplysia californica. J. Neurophysiol. 30 1288. 

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