Intracellular antibodies

Poznansky MC, La Vecchio J, Silva-Aiietta S, Porter-Brooks J, Brody K, Olszak IT, Adams GB, Ramstedt U, Marasco WA, Scadden DT (1999) Inhibition of human immunodeficiency vims replication and growth advantage of CD4+ T ceUs and monocytes derived from CD34-I- ceUs transduced with an intracellular antibody directed against human immunodeficiency vims type 1 Tat. Hum Gene Ther 10 2505-2514... 

One very new and powerful area of research is that of monoclonal intrabodies (also called intracellular antibodies), which represent a new class of targeting molecules with potential use in gene therapy. Due to their totally different targets, namely inside a cell, and thus different effector functions, they may have a high potential in the treatment of human diseases [6]. 

Lobato, M.N., andT.H. Rabbitts. 2004. Intracellular antibodies as specific reagents for functional ablation future therapeutic molecules. Curr. Mol. Med. 4 519-528. 

Rondon, I.J., and W.A. Marasco. 1997. Intracellular antibodies (intrabodies) for gene therapy of infectious diseases. 

Biocca S, Cattaneo A, Intracellular antibodies developments and applications, Austin, TX, Landes Bio science, distributed by Springer Verlag, 1997. 

Chen SY, Bagley J, Marasco WA (1994) Intracellular antibodies as a new class of therapeutic molecules for gene therapy. Hum Gene Ther 5 595-601. 

Richardson JH, Marasco WA (1995) Intracellular antibodies Development and therapeutic potential. Trends Biotechnol 13 306-310. 

Identification of new target molecules to assist intracellular antibody delivery... 

Identification of New Target Molecules to Assist Intracellular Antibody Delivery... 

A recognized flow cytometry procedure for standard indirect antibody staining for cellular analysis and cell sorting is discussed. A protocol for cell surface or intracellular antibody staining of viable cells is... 

Flow cytometry protocols for defining cell surface or intracellular antibody staining are discussed. Various staining protocols are provided. Routine cell surface and intracellular techniques as well as more advanced signal enhancement techniques are detailed. 

Noel, J., K. Fukami, A.M. Hill T. Capiod. 1992. Oscillations of cytosolic free calcium concentration in the presence of intracellular antibodies to phosphatidylinositol 4,5-bisphosphate in voltage-clamped guinea-pig hepatocytes. Biochem. J. 288 357-60. 

Zemel, R. et al. Inhibition of hepatitis C virus NS3-mediated cell transformation by recombinant intracellular antibodies. J. Hepatol. 2004, 40, 1000-1007. 

Tse E, Rabbitts TH. Intracellular antibody-caspase-mediated cell killing an approach for application in cancer therapy. Proc Natl Acad Sci USA 2000 97 12266 12271. 

Cattaneo A, Biocca S (1997) Intracellular antibodies development and applications. Springer, Berlin... 

Electroporated cells can be used only for short-term metabolic studies, as the intracellular antibody concentration remained intact only for 72 hr. 

For the assessment of intracellular cytokines in T cells, MNC are depleted of macrophages and monocytes and stimulated with mitogens (Protocol 6) in the presence of Brefeldin A, which inhibits newly S3mthesized protein from traversing the cell membrane. This allows the fluorescence signal form the chosen intracellular antibody-fluorochrome conjugate to be amplified. 

The brassinosteroids are essential for many growth and development processes in plants. However, relatively little is known about the effects of BRs and their synthetic analogs on animal cells [89]. It has been reported that cultured hybridoma mouse cells treated with 24-epiBL exhibit certain interesting effects, including an increase in the mitochondrial membrane potential, a decrease in the abundance of intracellular antibodies, an increase in the fraction of the cells in the Gq/Gi phase, and a corresponding decrease in the proportion of cells in S phase. Moreover, treatment with 24-epiBL at concentrations of 10 and 10 mol/1 significantly increased the density of viable cells relative to controls [90],... 

Deshane J, Siegal G, Alvarez R, et al. Targeted tumor killing via an intracellular antibody against erbB-2. J Clin Invest 1995 96 2980-2989. 

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