Cell imaging

Flenderson E, Flaydon P G and Sakaguchi D S 1992 Actin filament dynamics in living glial cells imaged by atomic force microscopy Science 257 1944... 

Optoelectronic components produced by CVD include semiconductor lasers, light-emitting diodes (LED), photodetectors, photovoltaic cells, imaging tubes, laser diodes, optical waveguides, Impact diodes, Gunn diodes, mixer diodes, varactors, photocathodes, and HEMT (high electron mobility transistor). Major applications are listed in Table 15.1.El... 

Radmacher, M., Tillman, R. W., Fritz, M., and Gaub, H. E., From Molecules to Cells Imaging Soft Samples with the Atomic Force Microscope," Science, Vol. 257, 1992, pp. 1900-1905. 

Huang, X., El-Sayed, I.H., Qian, W. and El-Sayed, M.A. (2006) Cancer cell imaging and photothermal therapy in the near-irrfrared region by using gold nanorods. Journal of the American Chemical Society, 128, 2115-2120. 

Fig. 7. Hypoxic cell imaging agents A) TcO (PnAO 1,2-nitroimidazole) B) Butyleneamineoxime...

Yano Y, Matsuzaki K (2009) Tag-probe labeling methods for live-cell imaging of membrane proteins. Biochim Biophys Acta-Biomembranes 1788 2124—2131... 

Wang M, Gao M, Miller KD et al (2009) Simple synthesis of carbon-11 labeled styryl dyes as new potential PET RNA-specific, living cell imaging probes. Eur J Med Chem 44 2300-2306... 

Villoing A, Ridhoir M, Cinquin B, Erard M, Alvarez L, Vallverdu G, Pemot P, Grailhe R, Merola F, Pasquier H (2008) Complex fluorescence of the cyan fluorescent protein comparisons with the FU48D variant and consequences for quantitative cell imaging. Biochemistry 47 12483-12492... 

The potential of live cell imaging to address mechanisms of cellular biology is ever expanding. Directed protein-tagging techniques have been used to visualize nascent versus mature protein in vivo (Rodriguez et al., 2006). This technique involves the use of arsenic-based dyes, such as FiAsH or ReAsH, which bind to tetracysteine (TC) tags (Zhang et al, 2002). In addition, photo-activatable variants of GFP have been shown to determine the kinetics of protein movement in live cells (Patterson and Lippincott-Schwarz, 2002). Furthermore, techniques such as FRET and the... 

Yudushkin, I. A., Schleifenbaum, A., Kinkhabwala, A., Neel, B. G., Schultz, C. and Bastiaens, P. I. H. (2007). Live-cell Imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B. Science 315, 115-119. 

Dixit, R., Cyr, R. and Gilroy, S. (2006). Using intrinsically fluorescent proteins for plant cell imaging. Plant J. 45, 599-615. 

BRET [31, 32]), lock-in detection techniques exploiting optical switches [33], and schemes for alternating D/A excitation (ALEX [34]). The increased attention to quantitative FRET imaging encompasses the use of polarization [35-39], the perennial issue of calibration and standards [40-44], and practical guides to operational principles and protocols ([45, 46] and other references above). The fundamental distinctions between the requirements for live and fixed cell imaging cannot be overemphasized, as is exemplified in a report of erroneous FRET determinations with visible fluorescent proteins (VFPs) in fixed cells [47],... 

Cell manipulation technique (e.g. cell imaging, gene transfection preservation, and differentiation)... 

Fig. 24 HER2/neu antibody-conjugated poly(amino acid)-coated iron oxide nanoparticles for breast cancer cell imaging. The part labeled as 1 is shown enlarged. (Adapted from [79])...

Fig. 6. Metallic imaging probes (a) and the fluorescence imaging (b) at the uptake of the Re(I) analog in tumor cells. Images adapted from Ref. (29).

Fig. 9. A fluorescent Re(I) complex (a) and its uptake in tumor cells imaged by fluorescence microscopy methods, (b). Images adapted from Ref. (33).

Brown, A. Live-cell imaging of slow axonal transport in cultured neurons. Methods Cell Biol. 71 305-323,2003. 

Los GV et al (2008) HatoTag a novel protein labeling technology for cell imaging and protein analysis. ACS Chem Biol 3 373-382... 

Niino Y, Hotta K, OkA K (2009) Simultaneous live cell imaging using dual FRET sensors with a single excitation light. PLoS One 4 e6036... 

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