Imaging of Live Cells

Campagnola P J, Wei M D, Lewis A and Loew L M 1999 High-resolution nonlinear optical imaging of live cells by second harmonic generation Biophys. J. 77 3341-9... [Pg.1305]

Although the idea on which NSOM is based goes back more than 50 years (32), D. W. Pohl first beheved it could be achieved with visible light and brought the concept to do it to fmition in 1984 (33). There is considerable interest in NSOM, and two commercial instmments have already been aimounced. A recent appHcation involves using NSOM for localized absorption spectroscopy and fluorescence imaging of living cells (33). [Pg.333]

Zal, T. and Gascoigne, N. R. (2004). Photobleaching-corrected FRET efficiency imaging of live cells. Biophys. J. 86, 3923-39. [Pg.359]

Jaiswal, J. K., Mattoussi, H., Mauro, J. M. and Simon, S. M. (2003). Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 21, 47-51. [Pg.479]

A. SPECT Imaging Reagents Suitable for the Fluorescence Imaging of Living Cells... [Pg.137]

Sekar RB, Periasamy A (2003) Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J Cell Biol 160 629-33... [Pg.131]

P.J. Campagnola, M.D. Wei, A. Lewis, and L.M. Loew, High-resolution nonlinear optical imaging of live cells hy second harmonic generation, Biophysical Journal 77(6), 3341-3349 (1999). [Pg.224]

The extraction of meaningful data from confocal images of living cells requires the establishment of fluorescent probe loading conditions for each specimen type. Consideration should be given to ... [Pg.157]

Vibrational imaging of living cells has been applied for studying intracellular lipid droplet organelles [86, 89, 90, 113, 114] (cf. Fig. 6.10A), monitoring receptor-mediated endocytosis [92], analyzing mouse embryonic stem cells... [Pg.127]

Fluorescence microscopy is one of the most powerful imaging methods in modern biomedical research. Noninvasive, fluorescence microscopy allows dynamic imaging of live cells, tissues, and whole organisms. This capability for live sample imaging, combined with a large repertoire of spectrally distinct fluorescent probes and a variety of biochemically specific labeling techniques, enables the direct visualization of complex molecular and cellular processes in real time under physiological conditions. [Pg.399]

Jamin, N., Dumas, P., Mincuit, )., Fridman, W.H., Teilland, J.L., Carr, G.L. and Williams, G.P. (1998) Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry. Proc. Natl Acad. Sci. USA, 95 (9), 4837-40. [Pg.256]

Massignani M, Canton 1, Sun T, Heamden V, MacNeil S, Blanazs A, Armes SP, Lewis A, Battaglia G(2009) Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes. Under review... [Pg.152]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...