Imaging Single Cells

2927cm (c), and carbohydrate image at 1025 cm (d) band area showing subaleurone endosperm. The units of the x- and y-axes are micrometers. Reproduced with permission from Refs [12, 17] Elsevier and Cellular and Molecular Biology, respectively. 

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Lipid Detection, Identification, and Imaging Single Cells with SIMS... 

Foster-Gareau, P., C. Heyn. et al. 2003. Imaging single cells with a 1.5T clinical MRI scanner. Magn Reson... 

In fact, this approach has been applied to image single cells trapped in droplets of Dulbecco s phosphate-buffered saline (DPBS) in oil with the potential to extend the methodology to study single cell dynamics as discussed in Section 9.3.5.2. 

Ribbe A E 1997 Laser scanning confocal microscopy in polymer science Trends Polym. Sc/. 5 333-7 Oliveira M J and Hemsiey D A 1996 Optical microscopy of polymers Sonderb. Prakt. Metallogr. 27 13-22 Nie Sh and Zare R N 1997 Optical detection of single molecules Ann. Rev. Biophys. Biomol. Struct. 26 567-96 Masters B R 1994 Confocal redox imaging of cells Adv. Mol. Cell Biol. 8 1-19... 

Image analysis in case of size change in organic material, pellets, mycelium hyphae or agglomerates of single cells (see e.g. [44 - 54,60])... 

Fig. 1. Optical image and SEM micrographs of a single cell (a) the SDC electrolyte surface view (b) LSM-SDC-NiSDC cross view.

A single cell layer of an excised piece of onion epidermis was selected as a test object for this micro-coil, (Figure 2.1.14), and an imaging experiment was carried out, similar to the one by Mansfield et al., who used a laboratory-made micro-coil probe and gradient system [31]. Other micro-coil types, e.g., volume coils or coils that are immersed into the objects, can be adapted to specific applications and mounted on commercial imaging probes. 

Kuimova MK, Botchway SW, Parker AW, Balaz M, Collins HA, Anderson HL, Suhling K, Ogilby PR (2009) Imaging intracellular viscosity of a single cell during photoinduced cell death. Nat Chem l(l) 69-73... 

Lord SJ, Conley NR, Lee HD, Nishimura SY, Pomerantz AK, Willets KA, Lu Z, Wang H, Liu N, Samuel R, Weber R, Semyonov A, He M, Twieg RJ, Moemer WE (2009) DCDHF fluorophores for single-molecule imaging in cells. ChemPhysChem 10 55-65... 

On September 13 2005 I received the invitation from professor P.C. van der Vliet (the editor of the Laboratory Techniques in Biochemistry and Molecular Biology Series) to become the editor of a new volume in the series on FRET and FLIM . In the letter it was mentioned that in view of the rapid developments in single cell technology, we feel that a book on imaging techniques in living cells, such as FRET and FFIM, is appropriate and timely . 

Harpur, A. G., Wouters, F. S. and Bastiaens, P. I. (2001). Imaging FRET between spectrally similar GFP molecules in single cells. Nat. Biotechnol. 19, 167-9. 

When coupled with an optical microscope, CL imaging is a potent analytical tool for the development of ultrasensitive enzymatic, immunohistochemistry (IHC) and in situ hybridization (ISH) assays, allowing spatial localization and semiquantitative evaluation of the distribution of the labeled probe in tissue sections or single cells to be performed. 

Most of the fluorescent calcium indicators and their cell-permeant acetoxymethyl (AM) esters are variations of the nonfluorescent calcium chelator BAPTA and have been proposed by Tsien/134-1365 Among them Fura-2 and Indo-1 (Figure 5.22) are particularly used formeasuring Ca2+in single cells by imaging or flow cytometry/65... 

When using isolated single cells, discrimination between binding and uptake is rather easily assessed by combining the confocal image with a usual transmission image. When the focus is set to the middle of the single cell,... 

Figure 4.1 The comet assay. A single-cell suspension is embedded in agarose on a slide. Cells are then subject to lysis followed by electrophoresis. If present, damaged DNA migrates out of the nucleoid structure during electrophoresis to producing a characteristic comet shape. Double-strand breaks are revealed under neutral conditions, whereas alkali conditions additionally show single-strand breaks and alkali labile sites. Image analysis of stained DNA is used to quantitate the amount of damaged DNA in the comet tail.

Loo, L.-H., Wu, L.-F. and Altschuler, S.J. (2007) Image-based multivariate profiling of drug responses from single cells. Nature Methods, 4, 445-453. 

Selective two-photon excitation can provide additional contrast for single-cell imaging. It might be particularly useful for co-localization studies. In Figure 8.8, it is illustrated on HeLa cells. The cells were co-stained with Phalloidin-350 (actin filaments) and with MitoTracker 488 (mitochondria). An amplitude shaping was used... 

Trace and Imaging Analysis on Biological Tissues and Single Cells... 

Of the mass spectrometric techniques, SIMS has become a useful tool for the imaging of single cells.174 Human liver tumor cells poisoned with Cd, Cu, Cr, Hg and Zn were analyzed using imaging ToF-SIMS (ION-TOF) to visualize the metal distributions on a single cell basis. Among the five metal ions investigated, only Cr and Cu showed a preferential diffusion into the cell after... 

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